In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Faculty of Natural Resources, University of Tehran, Iran

2 Faculty of Pharmacy, Medical Sciences, University of Tehran, Iran

3 Faculty of Agriculture, University of Buali Sina, Hamedan, Iran

Abstract

The essential oil yield and composition of the aerial parts of Diplotaenia cachrydifolia Boiss. were investigated in green and dry states, to understand why it is toxic and unpalatable in green state and palatable in dry state, for livestock. The essential oil was obtained by hydro-distillation method with Clevenger-type apparatus. GC and GC/MS analysis were carried out for investigating the oil composition. According to the results, the oil yield was 2.7% in green state and 0.4% in dry state. Ighteen components were identified representing 91.4% of the oil in green state and 17 components representing 91.6% of the oil in dry state. The major volatile components in green state were: limonene (49.6%), Cis-β-ocimene (12.6%), dill apiole (10.8%) and α-phellandrene (4.32%), respectively and in dry state were: limonene (50.7%), dill apiole (18.3%), Cis-β-ocimene (10.3%) and α-pinene (4.1%), respectively. The results showed that the high amount of the oil in green state is a factor for decreasing plant palatability. Furthermore, β-myrcene and α-phellandrene which are harmful biological compounds decreased in dry state. These compounds are introduced as forage anti-quality factors. In conclusion, the high quantity of volatile oil and some components are forage anti-quality factors.

Keywords

- سفیدکن، ف.، مشکی‌زاده، س. و علیها، م.، 1383. بررسی تغییرات کمی و کیفی اسانس Diplotaenia cachrydifolia در مراحل مختلف رشد. فصلنامه گیاهان دارویی، پژوهشکده گیاهان دارویی جهاد دانشگاهی، 3(11): 37-31.
- مظفریان، و.، 1385. فرهنگ نامهای گیاهان ایران. چاپ چهارم، فرهنگ معاصر، تهران، 596 صفحه.
- Abramov, A.Y., Zamaraeva, M.V., Hagelgans, A.I., Azimov, R.R. and Krasilnikov, O.V., 2001. Influence of plant terpenoids on the permeability of mitochondria a lipid bilayers. Journal of Biochimica et Biophysica Acta (BBA)/Biomembranes, 1512(1): 98-110.
- Adams, R.P., 2004. Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Corp., Carol Stream, USA, 456p.
- Budavari, S., 1996. The Merck Index. 12th ed, Merck & Co. INC, USA, 648p.
- Delgado, I.F., Carvalho, R.R., Nogueira, A.C., Mattos, A.P., Figueiredo, L.H., Oliverira, S.H., Ghahoud, I. and Paumgartten, F.J., 1993. Study of embryo-foetotoxicity of beta-myrcene in the rat. Journal of Chemical Research in Toxicology, 31: 31-35.
- De-Olivera, A.C., Ribeiro-Pinto, L.F., Otto, S.S., Goncalves, A. and Paumgartten, F.J., 1997. Induction of liver monooxygenases by beta-myrcene. Journal of Toxicology, 26: 135-140.
- Harkiss, K.J. and Surmaghy, M.H.S., 1987a. Volatiles from the root of Diplotaenia cachrydifolia, the first natural source of 6-camphenone. Journal of Natural Products, 50: 991-994.
- Harkiss, K.J. and Surmaghy, M.H.S., 1987b. Furocoumarins of the fruit and root of Diplotaenia cachrydifolia. Journal of Fitoterapia, 58: 409-410.
- Harkiss, K.J. and Surmaghy, M.H.S., 1988. Diplotaenia cachrydifolia, a new source of jatamansin and jatamansinol. Journal of Fitoterapia, 59: 55-56.
- Javidnia, K., Miri, R., Kamalinejad, M. and Mehdipour, A.R., 2006. Composition of the essential oil of Diplotaenia cachrydifolia Boiss. from Iran. Journal of Essential Oil Research, 18: 86-87.
- Ozcan, M., Bagci, Y., Ertugrul, K. and Novak, J., 2004. Comparison of the leaf, root and fruit oils of Diplotaenia cachrydifolia from Turkey. Journal of Essential Oil Research, 16: 211-213.
- Paumgartten, F.J., Delgado, I.F., Alves, E.N., Nogueira, A.C., De-Farias, R.C. and Neubert, D., 1990. Single dose toxicity study of beta-myrcene, a natural analgesic substance. Brazilian Journal of Medical and Biological Research, 23: 837-839.
- Zeinsteger, P., Romero, A., Teibler, P., Montenegro, M., Rios, E., Ciotti, E.M., Acosta De Perez, O. and Jorge, N., 2003. Toxicity of volatile compounds of Senecio grisebabachii baker (margarita) flowers in mice. Journal of RIA, 32(2): 125-136.