Document Type : Research Paper
Authors
1 Department of Immunology, Faculty of Medicine and Immunoregulation Research Group, Shahed University, Tehran, Iran
2 Department of Pharmacology, Faculty of Medicine and Herbal Medicine Research Center, Shahed University, Tehran, Iran
3 Shahed University, Tehran, Iran
Abstract
Immune system participates in etiology and pathophysiology of numerous diseases. Modulation of immune responses including humoral immune responses has been considered as a useful approach in control and disease treatments and immunomodulators can be effective in this regard. Herbal drugs are known as a source of immunomodulators. In this study the effect of MS14, a herbal – marine preparation, on humoral immune response in animal model is considered. MS14 have been orally administered (50 and 100 mg/kg) to 6-8 weeks old female Balb/C mice 5 days, the mice were immunized once using SRBC and were bled at day 6 (primary humoral response). Alternatively the mice were immunized multiple times during two weeks for secondary immune response in control group and MS14 (100 mg/kg). Serial dilutions of serum were prepared and agglutination test was performed using SRBC for primary and secondary sera. Serum IgG level in secondary sera has been determined by sandwich ELISA test. Antibody titer of primary serum was significantly reduced in agglutination test (the mean titer of antibody was 44.571 in control group and 27.6 in 50 mg/kg of MS14 and 7.33 in 100 mg/kg of MS14). Although, a little reduction has been observed in level of agglutinating antibody titer for secondary serum, but the reduction was not statistically significant (the mean titer of antibody was 25.6 in control group and 11.2 in MS14 group). Serum IgG level in control and MS14 group was not statistically significant as well (374.9 ng/ml in control and 382.1ng/ml in MS14 group). It can be concluded that MS14 possibly induces part of its immunomodulatory effect by reducing the production of IgM in primary humoral response but it has not any effect on IgG production in secondary humoral response.
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