Document Type : Research Paper
Authors
- Gh. Naderi 1
- N. Jafari Dinani 1
- N. Nejabat 2
- M. Kelardasht 3
- A. Jafarian Dehkordi 4
- S. Asgary 1
- R. Shami 4
1 Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
2 Islamic Azad University of Flavarjan, Iran
3 Member of young researchers Club, Islamic Azad University of Flavarjan, Iran
4 Isfahan University of Medical Sciences
Abstract
Disturbance in haemostatic system causes abnormal clots in vessels and thromboambolia. Streptokinas and urokinas are used for emergency treatment of thromboambolia. These drugs have certain limitations which cause serious and sometimes fatal consequences. Herbal preparations are considered to be safe and without side-effects. Since in ancient times it was reported that Zataria moltiflora Boiss., Curcuma domestica Valet., Cinnamomum verum J.Presl and Heracleum persicum Desf. Ex Fischer present anticoagulant effects. This study performed to evaluate fibrinolytic effects of these plants. To evaluate fibrinolytic effects, labeled fibrinogen with FITC and Ca2+ was added to human plasma. Polyphenolic extracts (0.05, 0.5, 5, 50mg/ml) without and with streptokinas, were added to labeled clot. Fluorescence was determined after 10, 20, 40 and 60 minutes (Ex=478, Em= 510). Our findings show that extract of Zataria moltiflora, Curcuma domestica and Cinnamomum verum reveal fibrinolytic effects (P<0.05). This effects are significant and dose dependable, but Heracleum persicum had no significant fibrinolytic effect (P>0.05). Fibrinolytic effects of streptokinase in presence Zataria moltiflora are increased significantly and dose dependably. This effect in presence of Curcuma domestica and Cinnamon only in 5 and 50 mg/dl are increased. Heracleum persicum does not affect fibrinolytic properties of streptokinase. The present study indicate that, Zataria moltiflora presents best fibrinolytic effect, then Curcuma domestica and Cinnamon verum respectively reveal significant fibrinolytic effect and could lysis blood clots in vitro. Consequently, we suggest that further studies to determine in vivo clot dissolving properties and active component(s) of these herbs for clot lysis are warranted.
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