Document Type : Research Paper

Authors

• K. Pirian ¹
• Kh. Piri ²

¹ MSc. Student, Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

² Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract

Portulaca oleracea L. as a medicinal plant, having valuable secondary metabolites, such as noradrenaline, dopamine and omega-3, is used as anti-cancer, antioxidant, and blood purifier factor. Callus inducted from hairy roots of some medicinal plants are used to increase the production of secondary metabolites, cell suspension culture, protoplast culture, induction of embryonic callus and gene transfer agents. In this experiment, various concentrations of two hormones of BA and 2,4-D were examined on transgenic hairy roots of P. oleracea to produce callus. Hairy roots transgenic of P. oleracea were induced by Agrobacterium rhizogene, 15834 strain. Hairy roots produced by Agrobacterium rhizogenes15834 strain, were transferred to 1/2MS medium containing different concentrations of BA and 2,4-D hormones. BA and 2,4-D hormones were used, both at three levels of 0, 0.5 and 1 mg per liter and in three replications. Our results indicated that BA and 2,4-D hormones alone as well as without hormone treatment (control) did not show any callus formation. The treatments containing different ratios of BA and 2,4-D hormones, caused callus  formation in varying degrees. The medium containing 1mg/L BA and 1 mg/L of 2,4-D hormones showed the higher production of callus.

Keywords

• Portulaca oleracea L
• Agrobacterium rhizogenes15834 strain
• Callus
• BA
• 2
• 4-D