Nitrogen effects on growth, biomass allocation, root and shoot alkaloids production  of black henbane (Hyoscyamus niger L.) under water decici stress

Ghorbanpour, M.`; Majnon Hossieni, N.; Rezazadeh, Sh.; Omidi, M.; Khavazi, K.; Hatami, M.; Ghafarzadegan, R.

doi:10.22092/ijmapr.2014.6124

Document Type : Research Paper

Authors

¹ Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran

² Department of Agronomy and Plant Breeding, Faulty of Agricultural Science, Tehran University, Karaj, Iran

³ Institute of Medicinal Plants (IMP), Karaj, Iran

⁴ Soil and Water Research Institute, Karaj, Iran

⁵ Department of Horticultural Sciences, Faculty of Agriculture, Guilan University, Rasht, Iran

⁶ Pharmaceutical Engineering Group, University of Tehran, Tehran, Iran

https://doi.org/10.22092/ijmapr.2014.6124

Abstract

In order to indicate the water deficit stress and nitrogen fertilization effects on alkaloids yield and content of Hyoscyamus niger L. plant parts (root and shoot), this experiment was conducted at the full flowering growth stage in greenhouse conditions. Plants were treated with different nitrogen application (0, 75, 150 and 225 kg/ha N as ammonium nitrate in the form of solution, N0-N3) before the commencement of water deficit stress treatment (30, 60 and 90% depletion of water from field capacity, W1-W3). Extracted alkaloids were analyzed by Gas chromatography /mass spectrometery (GC/MS) analysis using a Younglin Acme 6000 GC system equipped with a flame ionization detector (FID) and HP-5MS capillary column (30 m × 0.25 mm, film thickness 0.25 µm). The identification of alkaloids was based on the comparison of their GC retention time and mass spectra data with their standards substances. Results showed that the highest alkaloid content values in root (HYO: 0.281 %DW; SCO: 0.232 % DW) and shoot (HYO: 0.937%DW; SCO: 0.416%DW) were achieved in plants grown under sever water deficit stress (W3) accompanied with nitrogen supply of 225kg/h (N3). The maximum and minimum (20.52 and 8.95mg.plant^-1) total alkaloids yield in whole plant were obtained in N2W1 and N3W3 treatments, respectively. The survey results indicated that H. niger in the treatment of moderate water deficit stress (W2) along with 150kg N.h^-1 (N2) in addition to having a good amount of content and performance of both alkaloids, it also contained the highest level of SCO, indicating alkaloid quality.

Keywords

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Iranian Journal of Medicinal and Aromatic Plants Research

Nitrogen effects on growth, biomass allocation, root and shoot alkaloids production of black henbane (Hyoscyamus niger L.) under water decici stress

References

References

Volume 30, Issue 2 - Serial Number 64
June 2014
Pages 199-215

Nitrogen effects on growth, biomass allocation, root and shoot alkaloids production of black henbane (Hyoscyamus niger L.) under water decici stress

References

References

Volume 30, Issue 2 - Serial Number 64June 2014Pages 199-215

Volume 30, Issue 2 - Serial Number 64
June 2014
Pages 199-215