Document Type : Research Paper
Authors
1 Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran
2 M.Sc. student, Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran
3 Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Abstract
In the current research, aerial parts of Salvia verticillata L. were collected during the flowering stage from Esfehan-Khor and its chloroform extract was investigated. Purification of crude extract was carried out by column chromatography on silica gel with a gradient of n-hexan-ethyl acetate. At the end of chromatography, the column was eluted by methanol. Finally thirty-seven fractions were collected; further purification was carried out by column chromatography (smaller columns) and thin layer chromatography (glass plates) in n-hexane–ethyl acetate as solvent for several times and yielded pure compounds. The structure of purified natural products was elucidated by using Nuclear Magnetic Resonance (NMR) spectroscopy and Mass spectrometry. β-sitosterol and two diterpenoides namely Marrubinone B and Peregrinol were isolated.
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