Document Type : Research Paper
Authors
Abstract
This study was aimed at optimizing the medium composition of Ganoderma lucidum in submerged culture for efficient production of mycelial biomass and extracellular polysaccharides (EPS). The effects of various carbon sources (glucose, lactose and maltose), carbon source concentration (40, 50 and 60 g/l) and medium pH (3.5, 4.5 and 5.5) were studied on the production of mycelial biomass and EPS at 29±1°C. The experimental was factorial on the basis of randomized complete blocks design with 3 replications. The results showed that the highest (8.588 g DW/l) and the lowest (4.953 g DW/l) mycelial biomass were obtained in medium containing maltose and glucose respectively. Also, among the carbon sources, the medium containing maltose produced the highest (1.551 g/l) polysaccharide but the lowest polysaccharide (1.41 g/l) was produced in medium contacting lactose. No significant differences were observed among the different concentrations of carbon sources and the pH of the medium in terms of mycelial biomass and polysaccharides production. Finally, comparing the different combination of submerged culture media of Ganoderma lucidum revealed that the best combination of carbon source and pH for mycelial biomass production was maltose with concentration of 50 g/l and pH=4.5, while maltose with concentration of 40 g/l and pH=5.5 was identified as the best combination for EPS production.
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