Document Type : Research Paper
Authors
1 Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Prof., Department of Horticultural Science, Faculty of Agriculture, Urmia University, Urmia, Iran,
3 Department of Soil Science, Faculty of Agriculture, Urmia University, Urmia, Iran
4 Research Department of Chromatography, Iranian Academic Center for Education, Culture and Research (ACECR), Urmia, Iran
Abstract
Background and objectives: Basil (Ocimum basilicum L.) is an annual herbaceous plant belonging to the mint family (Lamiaceae). Its fresh and dried leaves and essential oils are used in the food, pharmaceutical, and cosmetic industries. In recent years, the use of symbiosis with plant beneficial rhizospheric microorganisms including rhizobacteria and endophytic fungi has been considered as a cost-efficient and sustainable strategy to alleviate the adverse effects of environmental stresses such as salinity. Therefore, the present study was conducted in the research greenhouse of Urmia University. It evaluated the effect of inoculation with growth-promoting microorganisms on some growth, physiological, and phytochemical characteristics of basil plants under salt-stress conditions.
Methodology: This research was conducted as a factorial experiment in a completely randomized design with three replications. The experimental factors were inoculation with microorganisms at three levels (control without inoculation, inoculation with Serendipita indica, and inoculation with a mixture of Pseudomonas areuginosa, P. putida, and P. fluorescens) and salinity stress at four levels (0, 40, 80 and 120 mM of NaCl). For inoculation, the germinated seeds were separately inoculated with S. indica suspension (5×105 spores per ml) and inoculum containing a mixture of Pseudomonas bacteria (1.61×109 cells per ml) and planted in prepared pots. The pots were irrigated with ordinary tap water until the plants were eight-leafed, and from this stage onwards, salinity stress treatments were applied by dissolving different concentrations of NaCl in the irrigation water and continued until the full flowering stage. In the full flowering stage, plant samples were collected and root colonization percentage by fungus, growth parameters (plant height, stem diameter, number and total length of lateral branches, leaf number and area, inflorescence length, fresh and dry weight of leaf and stem), leaf relative water content (RWC), photosynthetic pigments, concentration of leaf nutrients (N, P, K, Na and Cl), essential oil content and yield were evaluated. For essential oil extraction, shade-dried samples were hydro-distilled using a Clevenger-type apparatus.
Results: The results showed that due to salinity stress, the percentage of root colonization by S. indica, growth parameters, RWC, photosynthetic pigments, essential oil content, yield, N, P, and K content, and K/Na ratio decreased while Na and Cl content increased. In addition, all evaluated parameters in inoculated plants were higher than in non-inoculated plants except Na and Cl content. The highest and lowest rates of growth parameters, RWC (74.47 and 72.39%), essential oil yield (0.23 and 0.17 ml/pot), and N content of leaves (1.36 and 1.14%) were obtained in plants inoculated with S. indica and non-inoculated plants, respectively. The highest and lowest amounts of chlorophyll a (0.87 and 0.74 mg/g fw), chlorophyll b (0.41 and 0.37 mg/g fw), essential oil percentage (1.24 and 1.05%), K content (5.15 and 3.97%), P content (0.29 and 0.24%) and the K/Na ratio (10.05 and 4.07), were observed in plants inoculated with a mixture of Pseudomonas bacteria and non-inoculated plants, respectively. Also, the lowest Na (1.34%) and Cl (2.93%) accumulation was observed in plants inoculated with Pseudomonas bacteria and S. indica fungus, respectively.
Conclusion: According to the results of this study, the use of plant growth-promoting microorganisms (S. indica fungus and a mixture of Pseudomonas bacteria) can alleviate adverse effects of salinity stress on the growth and essential oil production of the basil plant by increasing the absorption of water and nutrients, preserving photosynthetic pigments and reducing the accumulation of toxic ions.
Keywords
- Essential oil
- Plant growth-promoting bacteria
- Salt stress
- Serendipita indica fungus
- Chlorophyll
- Relative water content
Main Subjects
- Acosta-Motos, J.R., Ortuño, M.F., Bernal-Vicente, A., Diaz-Vivancos, P., Sanchez-Blanco, M.J. and Hernandez, J.A., 2017. Plant responses to salt stress: Adaptive mechanisms. Agronomy, 7(1): 18.
- Akbari, T., Rostami, M., Ghabooli, M. and Movahedi, Z., 2021. Effect of Piriformospora indica on reducing the negative effects of salinity stress in lemon balm (Melissa officinalis). Journal of Crop Science Research in Arid Regions, 2(2): 219-229.
- Archangi, A. and Khodambashi, M., 2014. Effects of salinity stress on morphological characteristics, essential oil content and ion accumulation in basil (Ocimum basilicum) plant under hydroponic conditions. Journal of Science and Technology of Greenhouse Culture, 5(17): 125-138.
- Arvin, P. and Firouzeh, R., 2022. Effects of salinity stress on physiological and biochemical traits of some fenugreek (Trigonella foenum-graecum L.) populations. Iranian Journal of Medicinal and Aromatic Plants Research, 37(5): 822-837.
- Ashraf, M., 2004. Some important physiological selection criteria for salt tolerance in plants. Flora, 199: 361-376.
- Ashraf, M. and Akhtar, N., 2004. Influence of salt stress on growth, ion accumulation and seed oil content in sweet fennel. Biologia Plantarum, 48(3): 461-464.
- Ashraf, M., Hasnain, S., Berge, O. and Mahmood, T., 2004. Inoculating wheat seedling with exopolysaccharide-producing bacteria restricts sodium uptake and stimulates plant growth under salt stress. Biology and Fertility of Soils, 40(3): 157-162.
- Aslani, Z., Hassani, A., Abdollahi Mandoulakani, B., Barin, M. and Maleki, R., 2021. Effect of plant growth- promoting microorganisms inoculation on some growth and physiological parameters and nutrients content of sage (Salvia officinalis) under salinity stress conditions. Applied Soil Research, 9(3): 104-122.
- Azimzadeh, Z., Hassani, A., Abdollahi Mandoulakani, B. and Sepehr, E., 2021. Effects of salinity stress on some morpho-physiological characteristics, essential oil content, and ion relations of two oregano subspecies (Origanum vulgare ssp. vulgare & ssp. gracile). Iranian Journal of Medicinal and Aromatic Plants Research, 37(4): 658-674.
- Bahcesular, B., Yildirim, E.D., Karaçocuk, M., Kulak, M. and Karaman, S., 2020. Seed priming with melatonin effects on growth, essential oil compounds and antioxidant activity of basil (Ocimum basilicum L.) under salinity stress. Industrial Crops and Products, 146: 112165.
- Balasubramaniam, T., Shen, G., Esmaeili, N. and Zhang, H., 2023. Plants’ response mechanisms to salinity stress. Plants, 12(12): 2253.
- Bano, A. and Fatima, M., 2009. Salt tolerance in Zea mays (L). following inoculation with Rhizobium and Pseudomonas. Biology and Fertility of Soils, 45(4): 405-413.
- Bazyar, M., Bandehagh, A. and Farajzadeh, D., 2017. Effect of inoculation of Pseudomonas fluorescens FY32 bacteria to reduce the effects of salinity on canola (Brassica napus L.). Journal of Crop Production, 9(4): 201-220.
- Ben Taarit, M., Msaada, K., Hosni, K., Hammami, M., Kchouk, M.E. and Marzouk, B., 2009. Plant growth, essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Industrial Crops and Products, 30: 333-337.
- Bharti, N., Barnawal, D., Awasthi, A., Yadav, A. and Kalra, A., 2014. Plant growth promoting rhizobacteria alleviate salinity induced negative effects on growth, oil content and physiological status in Mentha arvensis. Acta Physiologiae Plantarum, 36: 45-60.
- Bonacina, C., Trevizan, C.B., Stracieri, J., dos Santos, T.B., Gonçalves, J.E., Gazim, Z.C. and de Souza, S.G.H., 2017. Changes in growth, oxidative metabolism and essential oil composition of lemon balm (Melissa officinalis L.) subjected to salt stress. Australian Journal of Crop Science, 11(12): 1665-1674.
- Chrysargyris, A., Michailidi, E. and Tzortzakis, N., 2018. Physiological and biochemical responses of Lavandula angustifolia to salinity under mineral foliar application. Frontiers in Plant Science, 489: 1-23.
- Çoban, Ö. and Baydar, N.G., 2016. Brassinosteroid effects on some physical and biochemical properties and secondary metabolite accumulation in peppermint (Mentha piperita L.) under salt stress. Industrial Crops and Products, 86: 251-258.
- Cotteni, A., 1980. Methods of plant analysis: 64-100. In: Westerman, R.L. (Ed.). Soil and Plant Testing. FAO Soil Bulletin, 784p.
- Dehghani Bidgoli, R., Azarnezhad, N., Akhbari, M. and Ghorbani, M., 2019. Salinity stress and PGPR effects on essential oil changes in Rosmarinus officinalis L. Agriculture and Food Security, 8: 1-7.
- Dell’Amico, E., Cavalca, L. and Andreoni, V., 2008. Improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biology and Biochemistry, 40(1): 74-84.
- Ebrahimi, Z., Morshedloo, M.R. and Hasanpour Aghdam, M.B., 2021. Study on physiological traits, percentage and essential oil components of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.) under salinity stress. Iranian Journal of Medicinal and Aromatic Plants Research, 37(4): 563-583.
- Egamberdieva, D., 2009. Alleviation of salt stress by plant growth regulators and IAA producing bacteria in wheat. Acta Physiologiae Plantarum, 31(4): 861-864.
- El-Keltawi, N.E. and Croteau, R., 1987. Salinity depression of growth and essential oil formation in spearmint and marjoram and its reversal by foliar applied cytokinin. Phytochemistry, 26: 1333-1334.
- Emami, A., 1996. Plant Analysis Methods. No. 982. Vol. 1. Soil and Water Research Institute Publication, Tehran, 128p.
- Emami Bistgani, Z., Hashemi, M., DaCosta, M., Craker, L., Maggi, F. and Morshedloo, M.R., 2019. Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Industrial Crops and Products, 135: 311-320.
- Enteshari, Sh. and Hajbagheri, S., 2011. Effects of mycorrhizal fungi on some physiological characteristics of salt stressed Ocimum basilicum L. Iranian Journal of Plant Physiology, 1(4): 215-222.
- Esmaielpour, B., Shiekhalipour, M. and Torabi-Giglo, M., 2020. Effects of zinc nanoparticles on growth, some physiological characteristics, and essential oil yield of Dracocephalum moldavica L. under salinity stress conditions. Iranian Journal of Medicinal and Aromatic Plant Research, 36(5): 867-884.
- Evelin, H., Kapoor, R. and Giri, B., 2009. Arbuscular mycorrhizal fungi in alleviation of salt stress: A review. Annals of Botany, 104(7): 1263-1280.
- Evelin, H., Giri, B. and Kapoor, R., 2013. Ultrastructural evidence for AMF mediated salt stress mitigation in Trigonella foenum-graecum. Mycorrhiza, 23: 71-86.
- Faghih Abdollahi, L., Pirdashti, H., Yaghoubian, Y. and Alavi, S.M., 2015. Effect of Piriformospora indica and Trichoderma tomentosum fungi on basil (Ocimum basilicum L.) growth under copper nitrate levels. Journal of Soil Management Sustainable Production, 5(1): 113-127.
- Farkhondeh, R., Nabizadeh, E. and Jalilnezhad, N., 2012. Effect of salinity stress on proline content, membrane stability and water relation in two sugar beet cultivars. International Journal of Agricultural Science, 2: 385-392.
- Farsaraei, S., Moghaddam, M. and Pirbalouti, A.G., 2020. Changes in growth and essential oil composition of sweet basil in response of salinity stress and superabsorbents application. Scientia Horticulturae, 271: 109465.
- Ghorbani, A., Razavi, S.M., Omran, V.O.G. and Pirdashti, H., 2018a. Piriformospora indica alleviates salinity by boosting redox poise and antioxidative potential of tomato. Russian Journal of Plant Physiology, 65(6): 898-907.
- Ghorbani, A., Razavi, S.M., Ghasemi Omran, V. and Pirdashti, H., 2018b. Piriformospora indica inoculation alleviates the adverse effect of NaCl stress on growth, gas exchange and chlorophyll fluorescence in tomato (Solanum lycopersicum L.). Plant Biology, 20(4): 729-736.
- Ghorbanpour, M., Hosseini, N., Khodae Motlagh, M. and Solgi, M., 2014. The effects of inoculation with Pseudomonas rhizobacteria on growth, quantity and quality of essential oils in Sage (Salvia officinalis L.) plant. Journal of Medicinal Plants, 13(52): 89-100.
- Gohari, G., Mohammadi, A., Akbari, A., Panahirad, S., Dadpour, M.R., Fotopoulos, V. and Kimura, S., 2020. Titanium dioxide nanoparticles (TiO2 NPs) promote growth and ameliorate salinity stress effects on essential oil profile and biochemical attributes of Dracocephalum moldavica. Scientific Reports, 10: 912.
- Gill, S.S., Gill, R., Trivedi, D.K., Anjum, N.A., Sharma, K.K., Ansari, M.W., Ansari, A.A., Johri, A.K., Prasad, R., Pereira, E., Varma, A. and Tuteja, N., 2016. Piriformospora indica: Potential and significance in plant stress tolerance. Frontiers in Microbiology, 7: 332.
- Glick, B.R., 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research, 169(1): 30-39.
- Hammer, E.C., Nasr, H., Pallon, J., Olsson, P.A. and Wallander, H., 2011. Elemental composition of arbuscular mycorrhizal fungi at high salinity. Mycorrhiza, 21: 117-129.
- Hao, S., Wang, Y., Yan, Y., Liu, Y., Wang, J. and Chen, S., 2021. A review on plant responses to salt stress and their mechanisms of salt resistance. Horticulturae, 7: 132.
- Hassanvand, F., Rezaei Nejad, A. and Fanourakis, D., 2019. Morphological and physiological components mediating the silicon-induced enhancement of geranium essential oil yield under saline conditions. Industrial Crops and Products, 134: 19-25.
- Heidari Sharif Abad, H., 2001. Plants and Salinity. Research Institute of Forests and Rangelands Publications, Tehran, 199p.
- Heidari, M., 2012. Effects of salinity stress on growth, chlorophyll content and osmotic components of two basil (Ocimum basilicum L.) genotypes. African Journal of Biotechnology, 11(2): 379-384.
- Homaei, M., 2002. Plant Response to Salinity. National Committee on Irrigation and Drainage Publications, 97p.
- Hu, Y. and Schmidhalter, U., 2005. Drought and salinity: A comparison of their effects on mineral nutrition of plants. Journal of Plant Nutrition and Soil Science, 168: 541-549.
- Jayasinghe, C., Gotoh, N., Aoki, T. and Wada, S., 2003. Phenolics composition and antioxidant activity of sweet basil (Ocimum basilicum L.). Journal of Agricultural and Food Chemistry, 51(15): 4442-4449.
- Jindal, V., Atwal, A. and Singh, R., 1993. Effect of Vesicular-arbuscular mycorrhizae on metabolism of moong plant under NaCl salinity. Plant Physiology, 31: 475-481.
- Jogawat, A., Saha, S., Bakshi, M., Dayaman, V., Kumar, M., Dua, M., Varma, A., Oelmüller, R., Tuteja, N. and Johri, A.K., 2013. Piriformospora indica rescues growth diminution of rice seedlings during high salt stress. Plant Signaling and Behavior, 8(10): e26891.
- Johnson, C.M. and Ulrich, A., 1975. Analytical methods for use in plant analysis. Bulletin of the California Agricultural Experiment Station, 78p.
- Kadian, N., Yadav, K., Badda, N. and Aggarwal, A., 2013. AM fungi ameliorates growth, yield and nutrient uptake in Cicer arietinum L. under salt stress. Russian Agricultural Sciences, 39(4): 321-329.
- Käfer, E., 1977. Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Advanced Genetics, 19: 33-131.
- Kapoor, R., Anand, G., Gupta, P. and Mandal, S., 2017. Insight into the mechanisms of enhanced production of valuable terpenoids by arbuscular mycorrhiza. Phytochemistry Reviews, 16(4): 677-692.
- Kaydan, D., Yagmur, M. and Okut, N., 2007. Effects of salicylic acid on the growth and some physiological characters in salt stressed wheat (Triticum aestivum L.). Tarim Bilimleri Dergisi, 13(2): 114-119.
- Keramati, S., Pirdashti, H.A., Babaezadeh, V.A. and Dahestani, A., 2017. Assessment of the effect of Piriformospora indica symbiosis and paclobutrazol application on growth parameters in sweet basil (Ocimum basilicum L.) in response to salinity stress. Journal of Plant Production, 24(2): 1-22.
- Khademian, R., Asghari, B., Sedaghati, B. and Yaghoubian, Y., 2019. Plant beneficial rhizospheric microorganisms (PBRMs) mitigate deleterious effects of salinity in sesame (Sesamum indicum L.): Physio-biochemical properties, fatty acids composition and secondary metabolites content. Industrial Crops and Products, 136: 129-139.
- Khair-ul-Bariyah, S., Ahmed, D. and Ikram, M., 2012. Ocimum basilicum: A review on phytochemical and pharmacological studies. Pakistan Journal of Chemistry, 2(2): 78-85.
- Khalvandi, M., Amerian, M., Pirdashti, H., Baradaran, M. and Golami, A., 2017a. Piriformospora indica symbiotic effect on the quantity and quality of essential oils and some physiological parameters of peppermint (Mentha piperita) under salt stress. Journal of Plant Process and Function, 6(21): 169-184.
- Khalvandi, M., Amerian, M., Pirdashti, H., Baradaran, M. and Golami, A., 2017b. Effects of Piriformospora indica fungi symbiotic on the quantity of essential oil and some physiological parameters of peppermint in saline conditions. Iranian Journal of Plant Biology, 32: 1-19.
- Khalvandi, M., Amerian, M., Pirdashti, H., Keramati, S. and Hosseini, J., 2019. Essential oil of peppermint in symbiotic relationship with Piriformospora indica and methyl jasmonate application under saline condition. Industrial Crops and Products, 127: 195-202.
- Lally, R.D., Galbally, P., Moreira, A.S., Spink, J., Ryan, D., Germaine, K.J. and Dowling, D.N., 2017. Application of endophytic Pseudomonas fluorescens and a bacterial consortium to Brassica napus can increase plant height and biomass under greenhouse and field conditions. Frontiers in Plant Science, 8: 2193.
- Lamian, A., Naghdi Badi, H., Ladan Moghadam, A. and Mehrafarin, A., 2015. Changes of morpho-physiological traits, essential oil and methyl chavicol content of Tarragon (Artemisia dracunculus) in response to mycorrhiza (Glomus intraradices) inoculation and salinity stress. Journal of Medicinal Plants, 14(56): 64-77.
- Lee, Y.C., Johnson, J.M., Chien, C.T., Sun, C., Cai, D., Lou, B., Oelmüller, R. and Yeh, K.W., 2011. Growth promotion of Chinese cabbage and Arabidopsis by Piriformospora indica is not stimulated by mycelium-synthesized auxin. Molecular Plant-Microbe Interactions, 24(4): 421-431.
- Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymology, 148: 350-382.
- Liu, W., Tan, M., Qu, P., Huo, C., Liang, W., Li, R., Jia, Y., Fan, X. and Cheng, C., 2022. Use of Piriformospora indica to promote growth of Strawberry daughter plants. Horticulturae, 8(5): 370.
- McMillen, B.G., Juniper, S. and Abbott, L.K., 1998. Inhibition of hyphal growth of a Vesicular arbuscular mycorrhizal fungus in soil containing sodium chloride limits the spread of infection from spores. Soil Biology and Biochemistry, 30(13): 1639-1646.
- Meena, K.K., Mesapogu, S., Kumar, M., Yandigeri, M.S., Singh G. and Saxena, A.K., 2010. Co-inoculation of the endophytic fungus Piriformospora indica with the phosphate-solubilising bacterium Pseudomonas striata affects population dynamics and plant growth in chickpea. Biology and Fertility of Soils, 46(2): 169-174.
- Meena, V.S., Meena, S.K., Verma, J.P., Kumar, A., Aeron, A., Mishra, P.K., Bisht, J.K., Pattanayak, A., Naveed, M. and Dotaniya, M.L., 2017. Plant beneficial rhizospheric microorganism (PBRM) strategies to improve nutrients use efficiency: a review. Ecological Engineering, 107: 8-32.
- Mohamed, H.I. and Gomma, E.Z., 2012. Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanus sativus) under NaCl stress. Photosynthetica, 50(2): 263-272.
- Mulvaney, R.L., 1996. Nitrogen-inorganic forms. 1123-1184. In: Sparks, D.L. (Ed.). Methods of Soil Analysis-Part 3. Chemical Methods-SSSA Book Series No. 5. Soil Science Society of America and American Society of Agronomy, Madison, 1390p.
- Munns, R., 2002. Comparative physiology of salt and water stress. Plant Cell Environment, 25: 239-250.
- Nadeem, S.M., Zahir, Z.A., Naveed, M. and Arshad, M., 2009. Rhizobacteria containing ACC deaminase confer salt tolerance in maize grown on salt-affected fields. Canadian Journal of Microbiology, 55(11): 1302-1309.
- Nagananda, G.S., Das, A., Bhattacharya, S. and Kalpana, T., 2010. In vitro studies on the effects of biofertilizers (Azotobacter and Rhizobium) on seed germination and development of Trigonella foenum-graecum L. using a novel glass marble containing liquid medium. International Journal of Botany, 6(4): 394-403.
- Naseem, H. and Bano, A., 2014. Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize. Journal of Plant Interactions, 9(1): 689-701.
- Parida, A.K., Das, A.B. and Mittra, B., 2004. Effects of salt on growth, ion accumulation, photosynthesis and leaf anatomy of the mangrove. Trees, 18(2): 167-174.
- Parida, A.K. and Das, A.B., 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60(3): 324-349.
- Philips, J. and Hayman, D., 1970. Improved procedures for cleaning roots and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55: 158-161.
- Purushothaman, B., Srinivasan, R.P., Suganthi, P., Ranganathan, B., Gimbun, J. and Shanmugam, K., 2018. A comprehensive review on Ocimum basilicum. Journal of Natural Remedies, 18(3): 71-85.
- Rajkumar, M., Bruno, L.B. and Banu, J.R., 2017. Alleviation of environmental stress in plants: the role of beneficial Pseudomonas spp. Critical Reviews in Environmental Science and Technology, 47(6): 372-407.
- Rasouli-Sadaghiani, M.H., Barin, M., Ashrafi-Saeidlou, S. and Shakouri, F., 2019. Effects of phosphate solubilizing microorganisms and mycorrhizal fungi on the growth parameters of corn (Zea mays L.) under salinity condition. Applied Soil Research, 7(3): 25-39.
- Salimi, A., Rowshan, V. and Khanpoor, E., 2017. Effect of salinity on quality and quantity of essential oil components and antioxidant activity in yarrow (Achillea millefolium L.). Iranian Journal of Medicinal and Aromatic Plants Research, 32(6): 948-957.
- Santos, C.V., 2004. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Scientia Horticulturae, 103: 93-99.
- Sarikhani, M.R. and Amini, R., 2020. Biofertilizer in sustainable agriculture: Review on the researches of biofertilizers in Iran. Agricultural Science and Sustainable Production, 30(1): 329- 365.
- Seraj, F., Pirdashti, H., Yaghoubian, Y. and Ghasemi Omran, V., 2016. The effect of Piriformospora indica inoculation on salt and drought stress tolerance in Stevia rebaudiana under in vitro conditions. Iranian Journal of Plant Biology, 29: 1-20.
- Seyed, M.A., Ayesha, S., Azmi, N., Al-Rabae, F.M., Al-Alawy, A.I., Al-Zahrani, O.R. and Hawsawi, Y., 2021. The neuroprotective attribution of Ocimum basilicum: a review on the prevention and management of neurodegenerative disorders. Future Journal of Pharmaceutical Sciences, 7(1): 1-14.
- Siddiqui, M.N., Mohammad, F., Khan, M.M.A. and Al-Whaibi, M.H., 2012. Cumulative effect of nitrogen and sulphur on Brassica juncea L. genotypes under NaCl stress. Protoplasma, 249: 139-153.
- Sirrenberg, A., Göbel, C., Grond, S., Czempinski, N., Ratzinger, A., Karlovsky, P., Santos, P., Feussner, I. and Pawlowski, K., 2007. Piriformospora indica affects plant growth by auxin production. Physiologia Plantarum, 131: 581-589.
- Talaat, N.B., Ghoniem, A.E., Abdelhamid, M.T. and Shawky, B.T., 2015. Effective microorganisms improve growth performance, alter nutrients acquisition and induce compatible solutes accumulation in common bean (Phaseolus vulgaris L.) plants subjected to salinity stress. Plant Growth Regulation, 75: 281-295.
- Touiss, I., Ouahhoud, S., Harnafi, M., Khatib, S., Bekkouch, O., Amrani, S. and Harnafi, H., 2021. Toxicological evaluation and hepatoprotective efficacy of Rosmarinic acid-rich extract from Ocimum basilicum L. Evidence-Based Complementary and Alternative Medicine, 2021: 6676998.
- Turner, N.C., 1981. Techniques and experimental approaches for the measurement of plant water status. Plant and Soil, 58: 339-366.
- Wu, C.H., Wood, T.K., Mulchandani, A. and Chen, W., 2006. Engineering plant-microbe symbiosis for rhizoremediation of heavy metals. Applied and Environmental Microbiology, 72: 1129-1134.
- Yang, J.W., Kloepper, J.W. and Ryu, C.M., 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science, 14: 1-4.
- Yu, Y., Xu, T., Li, X., Tang, J., Ma, D., Li, Z. and Sun, J., 2016. NaCl-induced changes of ion homeostasis and nitrogen metabolism in two sweet potato (Ipomoea batatas L.) cultivars exhibit different salt tolerance at adventitious root stage. Environmental and Experimental Botany, 129: 23-36.
- Zeng, J., Chen, A., Li, D., Yi, B. and Wu, W., 2013. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry, 61(24): 5720-5726.
- Zhao, S., Zhang, Q., Liu, M., Zhou, H., Ma, C. and Wang, P., 2021. Regulation of plant responses to salt stress. International Journal of Molecular Sciences, 22(9): 4609.
- Zrig, A., Tounekti, T., AbdElgawad, H., Hegab, M.M., Ali, S.O. and Khemira, H., 2016. Essential oils, amino acids and polyphenols changes in salt-stressed Thymus vulgaris exposed to open–field and shade enclosure. Industrial Crops and Products, 91: 223-230.
- Zolfaghari, M., Nazeri, V., Sefidkon, F. and Rejali, F., 2013. Effects of arbuscular mycorrhizal fungi on plant growth and essential oil content and composition of Ocimum basilicum L. Iranian Journal of Plant Physiology, 3(2): 643-650.
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