Iranian Journal of Medicinal and Aromatic Plants Research

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Evaluation of phytochemical, antioxidant, and inhibitory properties of Plantago major L. seeds hydroalcoholic extract on amyloid nanofibrils production

Document Type : Research Paper

Authors

1 Graduate student, Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, IRAN

2 Department of biology, Rasht Branch, Islamic Azad University, Rasht, Iran

3 Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, IRAN

Abstract

Plantago major L. is a herbaceous perennial plant with many biological effects such as strong antioxidant properties. This study aimed at investigating the inhibitory effect of P. major seeds extract on amyloid nanofibrils production and also to antioxidant properties of this extract. First, the plant powdered-seeds hydroalcoholic extract was prepared using 96% ethanol. The extract compounds were then analyzed by gas chromatography-mass spectrometry (GC/MS) method. The production of amyloid fibrils was confirmed using the concord spectrometry method and transmission electron microscope imaging and its antioxidant effects were evaluated by the DPPH method. The presence of pyrrolidinone (2.95%) and N-(4-aminobutyl) acetamide (17.98%) in the extract was confirmed by GC/MS. The highest antioxidant activity (99.18%) and lowest amyloid nanofibrils production (55.73%) were obtained in the concentrations of 2 and 0.4 mg.ml-1 of the extract, respectively. The presence of amyloid fibrils was confirmed by electron microscopic imaging. The results of this research showed that the P. major seeds extract can be introduced as a suitable candidate to reduce the complications caused by Alzheimer's disease.

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References
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Iranian Journal of Medicinal and Aromatic Plants Research
Volume 39, Issue 2
June 2023
Pages 203-212
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