Document Type : Research Paper

Authors

• T. Soleimani ¹
• A. Ebrahimi ²
• F. Mahjoubi ³
• M. Sadeghi ³

¹ Ph.D. student, Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran

³ Department of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Karaj, Iran

Abstract

Ginger (Zingiber officinale Roscoe.) is a spicy medicinal plant with antioxidant, antitumor, and anticancer properties. The present study was conducted to evaluate the effects of ethanol extract of fresh ginger rhizome on inhibiting HCT-116 colon cancer cells and the expression of TGFBR2 and DDC genes as tumor suppressor genes and β-Actin gene as reference gene. HPLC analysis was used to identify and measure the amount of 6-gingerol in the extract. Toxicity of different concentrations of the complete extract (0, 10, 50, 100, 150, 200, 250, 300, 350, 400, and 500 μg.ml^-1) on the HTC-116 cell line was investigated using the MTT test, 16 and 24 hours after the start of the test (at 16 and 24). The expression of TGFBT2, DCC, and β-Actin genes was assessed by RT-PCR after treatment with concentrations of 150 and 300 μg.ml^-1 of the complete extract at 16 and 24 hours. The amount of 6-gingerol was obtained 86.2 ± 2.03 mg per 100 g dry weight of ginger ethanol extract powder based on the HPLC results. The MTT test results showed that IC₅₀ was 80.44 at 16 h and 473.19 at 24 h. Cell mortality was significantly increased at concentrations of 150 and 300 μg.ml^-1 of the extract. Also, expression of the TGFBT2 and DCC genes increased at 150 μg.ml^-1 at both 24 and 16 hours significantly (P<0.01). The present research proved the ginger extract effect on tumor inhibitory genes induction in HCT-116 colon cancer cell line.

Keywords

• Ginger (Zingiber officinale Roscoe.)
• 6-gingerol
• Colorectal cancer
• HCT-116 cell line

20.1001.1.17350905.1401.38.3.5.7

Main Subjects

• Biological effects of essential oils and extracts