Effects of benomyl and cytokinin on in vitro contamination and growth of Vaccinium arctostaphylos L. node explants

Noruzpuor, M.; Zare, N.; Asghari Zakaria, R.; Sheikhzade, P.

doi:10.22092/ijmapr.2022.356225.3070

Document Type : Research Paper

Authors

¹ Ph.D. student, of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran

² Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran

https://doi.org/10.22092/ijmapr.2022.356225.3070

Abstract

Microbial contamination is one of the main challenges and influential factors in the response of explants under in vitro cultures, especially in woody plants where the explants are prepared from natural habitats. In the present study, to investigate the effects of benomyl on microbial contamination and growth of Vaccinium arctostaphylos L. node explants, MS containing different concentrations of benomyl (0-2000 mg.L^-1) was used. Furthermore, to investigate the interaction of benomyl, basal medium, and hormonal composition on the growth response of explants, different basal media (MS, AN, and WPM) containing IBA (0.1 mg.L^-1), cytokinin (Zeatin, BAP, and TDZ, each at concentrations of 0, 0.5, 1, and 2 mg.L^-1), and benomyl (0 and 150 mg.L^-1) were studied. The results showed that benomyl in MS medium had no significant effect on the explants contamination, but use of lower benomyl concentrations (400 mg.L^-1 or less) could provide better growth conditions. Interaction of treatments significantly affected the percentage of survival and explants foliation. MS contained benomyl showed higher percentage of survival and foliation than MS without benomyl, but in AN and WPM media, the percentage of survival and foliation were significantly decreased in the presence of benomyl. Furthermore, in AN and WPM media contained cytokinin, the presence of benomyl significantly reduced the foliation percentage, while in MS medium, the effect of benomyl was different depending on the concentration and cytokinin type.

Keywords

20.1001.1.17350905.1401.38.1.9.7

Main Subjects

Biotechnology

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Iranian Journal of Medicinal and Aromatic Plants Research

Effects of benomyl and cytokinin on in vitro contamination and growth of Vaccinium arctostaphylos L. node explants

References

References

Volume 38, Issue 1 - Serial Number 111
March 2022
Pages 150-165

Effects of benomyl and cytokinin on in vitro contamination and growth of Vaccinium arctostaphylos L. node explants

References

References

Volume 38, Issue 1 - Serial Number 111March 2022Pages 150-165

Volume 38, Issue 1 - Serial Number 111
March 2022
Pages 150-165