Document Type : Research Paper

Authors

• Z. Cheraghi ¹
• M. Yousefi ²
• Z. Habibi ¹
• S. Ghasemi ³

¹ Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, Tehran, Iran

² Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

³ Department of Chemistry, Ilam Branch, Islamic Azad University, Ilam, Iran

Abstract

Nepeta is one of the most important genus from the fam. lamiaceae with more than 280 species. In this study, N. haussknechtii Bornm. was collected at the flowering stage from Ardabil province, Iran and its chloroform extract was prepared. The chloroform crude extract was subjected to the successive column chromatography on the silica gel using n-hexane–ethyl acetate solvent gradient to yield 29 fractions. Further purification of the fraction No.5 resulted in the isolation of one known steroid namely β-sitosterol (1). In addition, the known triterpenoid oleanolic acid (2) was identified from the purification of the more polar fraction No.9. The compounds were assigned by the mass spectrometry, IR, ¹H NMR, ¹³C NMR, DEPT, and ¹H¹H COSY. Finally, the structures of compounds were confirmed by comparison of the spectral data with those described in the literature.

Keywords

• Phytochemistry
• Nepeta haussknechtii Bornm
• terpenoid
• β-sitosterol
• oleanolic acid

20.1001.1.17350905.1400.37.5.10.9

Main Subjects

• Phytochemistry (extraction, identification and measurement of active components)