Iranian Journal of Medicinal and Aromatic Plants Research

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DNA barcoding of some medicinal plants in eastern Golestan province

Document Type : Research Paper

Authors

1 M.Sc. Graduated, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

2 Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

3 Ph.D. graduated, Department of Biology, Faculty of Sciences, Gonbad Kavous University, Gonbad, Iran

4 Ph.D. graduated, Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran

Abstract

DNA barcoding technique is a useful tool for the identification of plant and animal species using a short and standard sequence of the genome. In the present study, this method was used to identify four plant species including Calendula persica C.A.Mey., Silybum marianum (L.) Gaertn., Satureja mutica Fisch. & C.A.Mey., and Malva neglecta Wallr. from the eastern Golestan province. The DNA was extracted by CTAB method and the PCR was performed with the primers designed based on the rbcL and trnH-psbA chloroplast barcodes and ITS nuclear barcode. The results of sequences were matched with the information in the NCBI database. The results showed that the all three barcodes were suitable for the samples studied due to their high resolution, low SNP number, and comprehensiveness in most species. Also, the barcodes comparison of the species collected from the rangelands and perfumeries showd that some plant species that are offered in the perfumeries are different from the plants that the natives use as medicine. It could be mentioned that the mistakes possibility in the medicinal plants offered in the perfumeries is undeniable. Therefore, the study on the other plant species in the perfumeries by the DNA barcoding method could be recommended as a necessity.

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References
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Iranian Journal of Medicinal and Aromatic Plants Research
Volume 37, Issue 5 - Serial Number 109
November and December 2021
Pages 838-849
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