Document Type : Research Paper
Authors
Biotechnology Department, Faculty of Biological Science, Alzahra University, Tehran, Iran
Abstract
The production of secondary metabolites varies under the different environmental conditions. The aim of this study was to investigate the effects of pH and temperature on the content of antioxidant compounds in a species of microscopic algae. First, the microalgal strain Monoraphidium sp. was cultured under the different temperature and pH treatments. The biochemical assays were then performed on the compounds such as photosynthetic pigments, phenolic compounds, and total flavonoids. The total antioxidant activity was evaluated by FRAP and DPPH methods. The results of this study showed that the microalgae Monoraphidium sp. had the maximum growth and high content of antioxidant compounds at the acidic pH and temperature 28°C. The results indicated that the optimal conditions can be achieved for the high production of antioxidant compounds in microalgae by changing the environmental factors, which can be used in the pharmaceutical, medical, and production of nutritional supplements industries.
Keywords
Main Subjects
- Ahmed, A., Jyothi, N. and Ramesh, A., 2016. Improved ammonium removal from industrial wastewater through systematic adaptation of wild type Chlorella pyrenoidosa. Water Science and Technology, 75(1): 182-188.
- Al-Farsi, M., Alasalvar, C., Morris, A. and Baron, M.G., 2005. Comparison of antioxidant activity, anthocyanins, carotenoid and phenolics of three native fresh and sun-dried date (Phoenix dactylifera L.) varieties grown in Oman. Journal of Agricultural and Food Chemistry, 53: 7592-7596.
- Bischoff, H.W. and Bold, H.C., 1963. Some algae from enchanted rock and related algal species. Phycological Studies, University of Texas, IV, Austin, 92p.
- Blinks, L.R., 1963. The effect of pH upon the photo synthesis of littoral marine algae. Journal Protoplasma. 57:126-136.
- Cardozo, K.H.M., Guaratini, T., Barros, M.P., Falcao, V.R., Tonon, A.P., Lopes, N.P., Campos, S., Torres, M.A., Souza, A.O., Colepicolo, P. and Pinto, E., 2007. Metabolites from algae with economical impact. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146: 60-78.
- Del Campo, J.A., Moreno, J., Rodrı́guez, H., Vargas, M.A., Rivas, J. and Guerrero, M.G., 2000. Carotenoid content of chlorophycean microalgae: factors determining lutein accumulation in Muriellopsis sp. (Chlorophyta). Journal of Biotechnology,76(1): 51-59.
- Ghasemi, Y., Moradian, A., Mohagheghzadeh, A.A., Shokravi, S. and Morowat, A., 2007. Antifungal and antibacterial activity of microalgae collected from paddy-fields of Iran: characterization of antimicrobial activity of Chroococcus dispersus. Journal of Biological Science, 7(4): 904-910.
- Gómez, P.I. and González, M.A., 2005. The effect of temperature and irradiance on the growth and carotenogenic capacity of seven strains of Dunaliellasalina (Chlorophyta) cultivated under laboratory conditions. Biological Research, 38(2-3): 151-162.
- Guedes, A., Amaro, M.H., Pereira, R.D. and Malcata, F.X., 2011. Effects of temperature and pH on growth and antioxidant content of the microalga Scenedesmus obliquus. Biotechnology Progress, 27: 1218-1224.
- Hajimahmoodi, M. Faramarzi, M.A., Mohammadi, N., Soltani, N., Oveisi, M.R. and Nafissi-Varcheh, N., 2010. Evaluation of antioxidant properties and total phenolic contents of some strains of microalgae, Journal of Applied Phycology, 23: 43-50.
- Han, F., Pei, H., Hu, W., Han, L., Zhang, Sh. and Ma, G., 2016. Effect of high temperature stress on microalgae at the end of the logarithmic phase for the efficient production of lipid. Environmental Technology, 37: 2649-2657.
- Han, J.W., Yoon, M., Kupper, F.D., Klockkova, T.A., Oh, J.R. and Kim, G.H., 2012. Accumulation of galloyl derivatives in a green alga, Spirogyra varinas, in response to cold stress. Journal of Applied Phycology, 24: 1279-1286.
- Hanachi, P., Zarringhalami, R. and Ramezani Tamijani, R., 2018. Investigation of antioxidant properties of Polygonatum orientale Desf and Tilia dasystyla extracts by different methods and solvents. Hormozgan Medical Journal, 22(4): e86504.
- Hanachi, P., Aghababaei, A. and Noroozi, M., 2019. The effect of pH and temperature on growth, the antioxidants, phenols and flavonoids in Scenedesmus sp. Microalgae. Journal of Fisheries (Iranian Journal of Natural Resources), 72(1): 13-27.
- Hejazi, M.A., de Lamarliere, C., Rocha, J.M.S., Vermue, M., Tramper, J.R. and Wijffels, R.H., 2002. Selective extraction of carotenoids from the microalga Dunaliella salina with retention of viability. Biotechnology and Bioengineering, 79: 29-36.
- Hejazi, M.A., Holwerda, E. and Wijffels, R.H., 2004. Milking microalga Dunaliella salina for β-carotene production in two-phase bioreactors. Biotechnology and Bioengineering, 85: 475-481.
- Ho, S.H., Ye, X., Hasunuma, T., Chang, J.Sh. and Kondo, A., 2014. Perspectives on engineering strategies for improving biofuel production from microalgae. BiotechnologyAdvances, 32: 1448-1459.
- Kumar, R.R., Rao, P.H., Subramanian, V.V. and Sivasubramanian, V., 2014. Enzymatic and non-enzymatic antioxidant potentials of Chlorella vulgaris grown in effluent of a confectionery industry. Journal of Food Science and Technology, 51(2): 322-328.
- Kurade, M.B., Kim, J.R., Govindwar, S.P. and Jeon, B.H., 2016. Insights into microalgae mediated biodegradation of diazinon by Chlorella vulgaris: Microalgal tolerance to xenobiotic pollutants and metabolism. Algal Research, 20: 126-134.
- Safi, C., Zebib, B., Merah, O., Pontalier, P.Y. and Vaca-Garcia, C., 2014. Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews, 35: 265-278.
- Shokravi, S., Soltani, N., Amirlatifi, F. and Safai, M., 2007. The effect of irradiance, CO2 availability, salt concentrations on some physiological characteristic of cyanobacterium Nostoc sp. Journal of Plant Science Researches, 3: 68-72.
- Singh, S.P. and Singh, P. 2015. Effect of temperature and light on the growth of algae species: A review. Renewable and Sustainable Energy Reviews, 50: 431-444.
- Soltani, N., Khavari-Nejad, R. and Shokravi, S., 2006. The effect of ammonium on growth and metabolism of soil cyanobacterium Ficherella sp. FS18. Journal of Plant Science Reasearches,1: 48-53.
- Wellburn, A., 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of Plant Physiology,
144: 307-313.
144: 307-313.
)