Iranian Journal of Medicinal and Aromatic Plants Research

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Cytotoxicity of Echium amoenum Fisch. et Mey. petals aqueous extract on human glioblastoma cells

Document Type : Research Paper

Authors

1 Biology Department, Shiraz University, Shiraz, Iran

2 M.Sc. graduated, Biology Department, Shiraz University, Shiraz, Iran

Abstract

Medicinal plants have potentially cytotoxic effects due to their secondary metabolites, and it is important to know their cytotoxicity. Echium amoenum Fisch. et Mey., native to the northern part of Iran, is one of the important medicinal herbs in traditional Iranian medicine. It has large red flowers that turn dark purple after drying, and its decoction has medicinal uses. The aim of this study was to evaluate the cytotoxicity of aqueous extract of E. amoenum petals on human cells. In this study, the cytotoxic effects of different concentrations of E. amoenum extract on human glioblastoma cells was studied by MTT assay in a completely randomized statistical design with three replications. The results showed that the aqueous extract of this plant with IC50=19.28 mg ml-1 had a cytotoxic effect on human glioblastoma cells. The morphology of the treated cells changed from polygonal to spherical. The results of the present study showed that although the aqueous extract of E. amoenum petals had beneficial effects, it should be used with caution according to the instructions, because as it has a cytotoxic effect on human glioblastoma cells, it may also have a cytotoxic effect on healthy cells. 

Keywords

References
- Abolhassani, M., 2004. Antibacterial effect of borage (Echium amoenum) on Staphylococcus aureus. Brazilian Journal of Infectious Diseases, 8(5): 382-385.
- Ait Mbarek, L., Ait Mouse, H., Elabbadi, N., Bensalah, M., Gamouh, A., Aboufatima, R., Benharref, A., Chait, A., Kamal, M. and Dalal, A., 2007. Anti-tumor properties of blackseed (Nigella sativa L.) extracts. Brazilian Journal of Medical and Biological Research, 40(6): 839-847.
- Berridge, M.V., Herst, P.M. and Tan, A.S., 2005. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnology Annual Review, 11: 127-152.
- Berridge, M.V. and Tan, A.S., 1993. Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence, and involvement of mitochondrial electron transport in MTT reduction. Archives of Biochemistry and Biophysics, 303(2): 474-482.
- Chang, H.F., Huang, W.T., Chen, H.J. and Yang, L.L., 2010. Apoptotic effects of γ-mangostin from the fruit hull of Garcinia mangostana on human malignant glioma cells. Molecules, 15(12): 8953-8966.
- Denizot, F. and Lang, R., 1986. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods, 89(2): 271-277.
- Etebari, M., Zolfaghari, B., Jafarian-Dehkordi, A. and Rakian, R., 2012. Evaluation of DNA damage of hydro-alcoholic and aqueous extract of Echium amoenum and Nardostachys jatamansi. Journal of research in medical sciences, 17(8): 782-786.
- Firoozan, J., 2019. Evaluation of cytotoxic effect of Echium amoenum Fisch & C.A. Mey. and Cuscuta epithymum extracts on breast cancer cells in in vitro system. Dissertation, Tabriz University of Medical Sciences, 18: 825-831.
- Fotakis, G. and Timbrell, J.A., 2006. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters, 160(2): 171-177.
- Grecco, S.D.S., Martins, E.G.A., Girola, N., de Figueiredo, C.R., Matsuo, A.L., Soares, M.G., Bertoldo, B.D.C., Sartorelli, P. and Lago, J.H.G., 2015. Chemical composition and in vitro cytotoxic effects of the essential oil from Nectandra leucantha leaves. Pharmaceutical Biology, 53(1): 133-137.
- Hayon, T., Dvilansky, A., Shpilberg, O. and Nathan, I., 2003. Appraisal of the MTT-based assay as a useful tool for predicting drug chemosensitivity in leukemia. Leukemia & Lymphoma, 44(11): 1957-1962.
- Heidari, M.R., Azad, E.M. and Mehrabani, M., 2006. Evaluation of the analgesic effect of Echium amoenum Fisch & C.A. Mey. extract in mice: possible mechanism involved. Journal of Ethnopharmacolgy, 103(3): 345-349.
- Iselt, M., Holtei, W. and Hilgard, P., 1989. The tetrazolium dye assay for rapid in vitro assessment of cytotoxicity. Arzneimittel-Forschung, 39(7): 747-749.
- Khan, M., Xiao, Y., Yu, B., Wang, N., Rasul, A., Yi, F., Yang, L., Yang, H., Ma, T. and Artabotryside, A., 2012. A constituent from Descurainia sophia (L.) induces cell death in U87 glioma cells through apoptosis and cell cycle arrest at G2/M phase. Journal of Medicinal Plants Research, 6(21): 3754-3765.
- Khanavi, M., Pourmoslemi, S., Farahanikia, B., Hadjiakhoondi, A. and Ostad, S.N., 2010. Cytotoxicity of Vinca minor. Pharmaceutical Biology, 48(1): 96-100.
- Mehrabani, M. and Mehrabani, M., 2007. Evaluation of hepatotoxicity of common doses of decoction of Echium amoenum Fisch and CA Mey in rats. Journal of Kerman University of Medical Sciences, 14(1): 44-54.
- Mehrabani, M., Ghannadi, A., Sajjadi, S.E., Ghassemi, N. and Shams-Ardakani, M., 2006. Toxic pyrrolizidine alkaloids of Echium amoenum Fisch and Mey. Daru, 14: 122-127.
- Mohammadpour, G., Tahmasbpour, R., Noureini, S., Tahmasbpour, E. and Bagherpour, G., 2015. In vitro antimicrobial and cytotoxicity assays of Satureja bakhtiarica and Zataria multiflora essential oils. American Journal of Phytomedicine and Clinical Therapeutics, 3(6): 502-511.
- Rabbani, M., Sajjadi, S., Vaseghi, G. and Jafarian, A., 2004. Anxiolytic effects of Echium amoenum on the elevated plus-maze model of anxiety in mice. Fitoterapia, 75(5): 457-464.
- Sayyah, M., Boostani, H., Pakseresht, S. and Malaieri, A., 2009. Efficacy of aqueous extract of Echium amoenum in treatment of obsessive-compulsive disorder. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 33(8): 1513-1516.
- Silva, D.S., Garcia, A.C., Mata, S.S., Oliveira, B.D., Estevam, C.S., Scher, R. and Pantaleao, S.M., 2011. Genotoxicity and cytotoxicity of Erythrina velutina Willd. Fabaceae, on the root meristem cells of Allium cepa. Revista Brasileira de Farmacognosia, 21(1): 92-97.
- Stegelmeier, B.L., 2011. Pyrrolizidine alkaloid-containing toxic plants (Senecio, Crotalaria, Cynoglossum, Amsinckia, Heliotropium, and Echium spp.). Veterinary Clinics: Food Animal Practice, 27(2): 419-428.
- Tapsell, L.C., Hemphill, I., Cobiac, L., Sullivan, D.R., Fenech, M., Patch, C.S., Roodenrys, S., Keogh, J.B., Clifton, P.M. and Williams, P.G., 2006. Health benefits of herbs and spices: the past, the present, the future. Medical Journal of Australia, 185(4): S4-24.
- Timothy, S.T. and Richard, L.K., 2007. Herbal Products, Toxicology and Clinical Pharmacology. New Jersey: Humana Press, 288p.
- Vivek, R., Thangam, R., Muthuchelian, K., Gunasekaran, P., Kaveri, K. and Kannan, S., 2012. Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells. Process Biochemistry, 47(12): 2405-2410.
- Yao, T. and Asayama, Y., 2017. Animal-cell culture media: History, characteristics, and current issues. Reproductive Medicine and Biology, 16(2): 99-117.
- Yuan, Q., Zhang, X., Liu, Z., Song, S., Xue, P., Wang, J. and Ruan, J., 2013. Ethanol extract of Adiantum capillus-veneris L. suppresses the production of inflammatory mediators by inhibiting NF-κB activation. Journal Ethnopharmacology, 147(3): 603-611.
Iranian Journal of Medicinal and Aromatic Plants Research
Volume 36, Issue 3 - Serial Number 101
July and August 2020
Pages 438-447
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