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Influence of drought stress and plant growth promoting rhizobacteria (PGPR) inoculation on morphological characteristics, essential oil yield and composition of Thymus daenensis Clack

Document Type : Research Paper

Authors

1 Soil Sciences Department, Agriculture Faculty, Lorestan University, Khurramabad, Iran

2 Soil Sciences Department, Agriculture Faculty, Lorestan University, Khorramabad, Iran

3 Soil Sciences Department, Agriculture Faculty, Isfahan University of Technology, Isfahan, Iran

Abstract

To investigate the effects of plant growth promoting rhizobacteria (PGPR) and drought stress on essential oil of Thymus daenensis Clack, a greenhouse experiment was conducted in Shahrekord, Iran in 2017. The experiment was arranged as a factorial in a completely randomized design with three replications. The first factor included four levels of irrigation: well-watered (No stress), irrigation after depletion of 20-25% of field capacity (FC) (Low stress), irrigation after depletion of 35-40% of FC (Mild stress) and irrigation after depletion of 55-60% of FC (Severe stress). The second factor included two levels of PGPR treatments: no inoculation (Control) and inoculation with PGPR. The results showed that the morphological parameters were significantly increased in PGPR treatments, while water stress decreased all parameters. The essential oil amount increased in low stress and decreased in severe stress. PGPR inoculation increased the amount of essential oil, although this increase was not statistically significant. PGPR incubation and drought stress had a significant effect on 13 and 14 components, respectively. The interaction of PGPR and drought stress had a significant effect on the oil components. Thymol and carvacrol, two important components of T. daenensis essential oil, decreased with increasing severity of drought stress, but PGPR inoculation increased them, especially at drought stress treatments.

Keywords

References
- Abdollahi Arpanahi, A. and Feizian, M., 2019. Variation in the essential oil composition and morphological parameters of Thymus daenensis Clack with two species of mycorrhizal fungi under water deficit conditions. Journal of Essential Oil Bearing Plants, 22(3): 675-684.
- Alavi-Samani, S.M., Ghasemi Pirbalouti, A., Ataei Kachuei, M. and Hamedi, B., 2013. The influence of reduced irrigation on herbage, essential oil yield and quality of Thymus vulgaris and Thymus daenensis. The International Journal of Herbal Medicine, 4(3): 109-113.
- Alavi-Samani, S.M., Kachouei, M.A. and Pirbalouti, A.G., 2015. Growth, yield, chemical composition, and antioxidant activity of essential oils from two thyme species under foliar application of Jasmonic acid and water deficit conditions. Horticulture, Environment, and Biotechnology, 56(4): 411-420.
- Bahadori, F., Sharifi Ashorabadi, E., Mirza, M., Matinizade, M. and Abdosi, V., 2013. Improved growth, essential oil yield and quality in Thymus daenensis Celak on mycorrhizal and plant growth promoting Rhizobacteria inoculation. International Journal of Plant Production, 4(12): 3384-3391.
- Bahadur, A., Singh, U.P., Sarma, B.K., Singh, D.P., Singh K.P. and Singh, A., 2007. Foliar application of plant growth-promoting Rhizobacteria increases antifungal compounds in pea (Pisum sativum) against Erysiphe pisi. Mycobiology, 35(3): 129-134.
- Bahreininejad, B., Razmjoo, J. and Mirza, M. 2013. Influence of water stress on morpho-physiological and phytochemical traits in Thymus daenensis. International Journal of Plant Production, 7: 155-166.
- Banchio, E., Bogino, P.C., Zygadlo, J. and Giordano, W., 2008. Plant growth promoting rhizobacteria improve growth and essential oil yield in Origanum majorana L. Biochemical Systematics and Ecology, 36: 766-771.
- Banchio, E., Xie, X. Zhang, H. and PAR, P.W., 2009. Soil bacteria elevate essential oil accumulation and emissions in sweet basil. Journal of Agricultural and Food Chemistry, 57: 653-657.
- Barea, J., Pozo, M., Azcon, R. and Azcon-Aguilar, C., 2005. A microbial co-operation in the rhizosphere. Journal of Experimental Botany, 56(417): 1761-1778.
- Cappellari, L., Santoro, M.V., Nievas, F., Giordano, W. and Banchio, E., 2013. Increase of secondary metabolite content in marigold by inoculation with plant growth-promoting rhizobacteria. Applied Soil Ecology, 70: 16-22.
- Cappellari, L., Santoro, M.V., Reinoso, H., Travaglia, C., Giordano, W. and Banchio, E., 2015. Anatomical, morphological, and phytochemical effects of inoculation with plant growth- promoting Rhizobacteria on peppermint (Mentha piperita). Journal of Chemical Ecology, 41(2): 149-158.
- Ghasemi Pirbalouti, A., Hashemi, M. and Taherian Ghahfarokhi, F., 2013. Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products, 48: 43-48.
- Ghasemi Pirbalouti, A., Samani, M.R., Hashemi, M. and Zeinali, H., 2014. Salicylic acid affects growth, essential oil and chemical compositions of thyme (Thymus daenensis Celak) under reduced irrigation. Plant Growth Regulation, 72: 289-301.
- Ghorbanpour, M., Hatami, M. and Khavazi, K., 2013. Role of plant growth promoting rhizobacteria on antioxidant enzyme activities and tropane alkaloid production of Hyoscyamus niger under water deficit stress. Turkish Journal of Biology, 37: 350-360.
- Ghorbanpour, M., Hatami, M., Kariman, K. and Khavazi, K., 2015. Enhanced efficiency of medicinal and aromatic plants by PGPRs: 43-70. In: Egamberdieva, D., Shrivastava, S. and Varma, A., (Eds.). Plant-Growth-Promoting Rhizobacteria (PGPR) and Medicinal Plants. Springer International Publishing, Switzerland, 442p.
- Glick, B.R., 1995. The enhancement of plant growth by free-living bacteria. Canadian Journal of Microbiology, 41: 109-117.
- Gray, E.J. and Smith, D.L., 2005. Intracellular and extracellular PGPR: commonalities anddistinctions in the plant-bacterium signalling processes. Soil Biology and Biochemistry, 37: 395-412.
- Jamzad, Z., 2009. Thymus and Satureja species of Iran. Research Institute of Forests and Rangelands, 172p.
- Liddycoat, S.M., Greenberg, B.M. and Wolyn, D.J., 2009. The effect of plant growth-promoting rhizobacteria on Asparagus seedlings and germinating seeds subjected to water stress under greenhouse conditions. Canadian Journal of Microbiology, 55: 388-394.
- Mohammadi, H., Dashi, R., Farzaneh, M., Parviz, L. and Hashempour, H., 2016. Effects of beneficial root pseudomonas on morphological, physiological, and phytochemical characteristics of Satureja hortensis (Lamiaceae) under water stress. Brazilian Journal of Botany, 40: 41-48.
- Mohammadi, M., Khavazi, K., Malakouti, M.J. and Rejali, F., 2017. Improve the quality of two cultivars of bean (Phaseolus vulgaris L.) with using Phosphate and Zinc biofertilizers. Iranian Journal of Pulses Research, 8(2): 44-56.
- Mohammadi, M., Malakouti, M.J., Khavazi, K., Rejali, F. and Davoodi, M.H., 2015. The effect of bio-fertilizer and chemical fertilizers (phosphate and zinc) on yield and yield components of two cultivars of bean (Phaseolus vulgaris L.). Journal of Water and Soil, 29(1): 176-187.
- Mahmoudzade, M., Rasouli Sedghiani, M.H. and Asagari Lajayer, H., 2016. Effect of plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi on growth characteristics and concentration of macronutrients in peppermint (Mentha pipperita L.) under greenhouse conditions. Journal of Science and Technology of Greenhouse Culture, 6(24): 155-167.
- Naghdi Badi, H. and Makizade Tafti, M., 2003. An overview of Thymus vulgaris L. Journal of Medicinal Plants, 2(7): 1-12.
- Ndeddy Aka, R.J. and Babalola, O.O., 2016. Effect of bacterial inoculation of strains of pseudomonas aeruginosa, alcaligenes feacalis and bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. International Journal of Phytoremediation, 18(2), 200-209.
- Nickavar, B., Mojab, F. and Dolat-Abadi, R., 2005. Analysis of the essential oils of two Thymus species from Iran. Food Chemistry, 90: 609-611.
- Rasipour, L. and Aliasgharzadeh, N., 2007. Interactive Effect of Phosphate Solubilizing Bacteria and Bradyrhizobium japonicum on Growth, Nodule Indices and Some Nutrient Uptake of Soybean. Journal of Water and Soil Science, 11(40): 53-64.
- Roumani, A., Ehteshami, S.M.R. and Rabiei, M., 2014. Effect of seed inoculation with Plant Growth Promoting Rhizobacteria (PGPR) on forage quality and yield of turnip (Brassica rapa L.) at the different values of nitrogen and phosphorus fertilizers. Plant Production Technology, 14(2): 89-99.
- Simon, J.E., Reiss-Bubenheim. D., Joly. R.J. and Charles, D.J., 1992. Water stress-induced alterations in essential oil content and composition of sweet basil. Journal of Essential Oil Research, 4:71-75.
- Santoro, M.V., Zygadlo, J., Giordano, W. and Banchio, E., 2011. Volatile organic compounds from rhizobacteria increase biosynthesis of essential oils and growth parameters in peppermint (Mentha piperita). Plant Physiology and Biochemistry, 49: 1177-1182.
- Selmar, D. and Kleinwachter, M., 2013. Stress enhances the synthesis of secondary plant products: the impact of the stress-related overreduction on the accumulation of natural products. Plant and Cell Physiology, 54: 817-826.
- Singh, U.P., Sarma, B.K. and Singh, D.P., 2003. Effect of plant growth-promoting rhi-zobacteria and culture filtrate of Sclerotium rolfsii on phenolic and salicylic acidcontents in Chickpea (Cicer arietinum). Current Microbiology, 46: 131-140.
- Stahl-Biskup, E. and Saez, F., 2002. Thyme the genus Thymus. Taylor & Francis, 352p.
- Tahami, M.K., Jahan, M., Khalilzadeh, H. and Mehdizadeh, M., 2017. Plant growth promoting rhizobacteria in an ecological cropping system: A study on basil (Ocimum basilicum L.) essential oil production. Industrial Crops and Products, 107:
97-104.
- Van Loon, L.C., 2007. Plant response to plant growth-promoting Rhizobacteria. European Journal of Plant Pathology, 119: 243-254.
- Vessey, J.K., 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255: 571-586. 
Iranian Journal of Medicinal and Aromatic Plants Research
Volume 36, Issue 3 - Serial Number 101
July and August 2020
Pages 417-428
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