In vitro evaluation for salinity tolerance in Cynara scolymus L.

Shahraki, M.A.; Emamjomeh, A.; Valizadeh, M.; Fahmideh, L.

doi:10.22092/ijmapr.2020.126493.2573

Document Type : Research Paper

Authors

¹ M.Sc. graduated, Department of Green Space Engineering, University of Zabol, Zabol, Iran

² Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran; Department of Bioinformatics, University of Zabol, Zabol, Iran

³ Department of Medicinal Plants, Faculty of Life Science and Sustainable Agriculture, University of Sistan and Baluchestan, Zahedan, Iran

⁴ Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran

https://doi.org/10.22092/ijmapr.2020.126493.2573

Abstract

Cynara scolymus L. is an economically valuable medicinal plant, but salinity can limit the regions under cultivation of this crop. Therefore, identifying the appropriate tissue culture method for this plant can be useful for selecting salinity tolerant cultivars. This research was carried out with the aim of identifying the appropriate explant and method for tissue culture of this plant as the first step to produce tolerant cultivars in future projects. In this study, firstly, sterile seedlings of plant seeds were prepared. Then, the terminal meristem was selected as a suitable explant during a pre-test and evaluated in vitro to study salinity tolerance with five different concentrations of sodium chloride (0, 20, 40, 60, and 80 mM) in a completely randomized design with three replications. This research was performed in the medicinal plants research center of Sistan and Baluchistan University in 2017. Salinity reduced some morphological traits (stem length, root length, shoot and root fresh and dry weight) of seedlings grown from terminal meristem explant. As the concentration of sodium chloride increased to 60 and 80 mM, the amount of soluble sugars and proline increased. The lowest amount of soluble sugars (33 mg g^-1 dry weight) and proline (50 µM) was related to zero and 20 mM sodium chloride concentrations, respectively. The results indicate that this plant is sensitive to different levels of salinity, although it is somewhat tolerant to low and moderate levels of salinity.

Keywords

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Iranian Journal of Medicinal and Aromatic Plants Research

In vitro evaluation for salinity tolerance in Cynara scolymus L.

References

References

Volume 36, Issue 2 - Serial Number 100
May and June 2020
Pages 291-304

In vitro evaluation for salinity tolerance in Cynara scolymus L.

References

References

Volume 36, Issue 2 - Serial Number 100May and June 2020Pages 291-304

Volume 36, Issue 2 - Serial Number 100
May and June 2020
Pages 291-304