Effects of explant type on callogenesis and the amount of betulin and betulinic acid produced under in vitro conditions in two birch species (Betula spp.)

Payamnoor, V.; Nazari, J.; Jafari Hajati, R.

doi:10.22092/ijmapr.2019.124949.2478

Document Type : Research Paper

Authors

¹ Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

² Ph.D. student, Faculty of Forest Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

³ Research Center for Clinical Trials of Traditional Medicine, Shahed University, Tehran, Iran

https://doi.org/10.22092/ijmapr.2019.124949.2478

Abstract

Betulin and betulinic acid are from the most important anticancer and anti-HIV metabolites, and the birch species (Betula spp.) bark is considered as the primary source of these metabolites. Due to the extinction of these tree species in Iran, it is necessary to replace the metabolites extraction from the birch bark with modern methods such as cell and tissue culture to produce the metabolites. The aim of this study was to determine the effect of explant type on the amount of betulin and betulinic acid produced in calli of two birch species B. pendula and B. litwinowii under in vitro conditions compared to the amount of metabolites extracted from the tree bark. Bark and leaf explants of two mentioned species were cultured in WPM medium containing 1 mg/L BAP and 0.1 mg/L 2,4-D for callogenesis. The amount of betulin and betulinic acid in three-month calli was measured using the HPLC technique and compared with the amount of these metabolites in one-centimeter stem bark samples taken from nature. The bark explant was more successful in callogenesis, and calli derived from this explant had more active ingredients. The amount of betulin and betulinic acid from the extract of bark sample taken from nature was respectively obtained to be 5.23 and 2.91 percent for B. pendula, and 5.65 and 2.52 percent for B. litwinowii. Moreover, calli derived from the bark explant of B. pendula and B. litwinowii contained 0.023 and 0.016 percent of betulin and 0.053 and 0.057 percent of betulinic acid, respectively. Generally, the results indicated that the bark explant was more capable of callogenesis and secondary metabolite induction than the leaf explant in both birch species under in vitro conditions.

Keywords

References

- Abyshev, A.Z., Agaev, E.M. and Guseinov, A.B., 2007. Studies of the chemical composition of birch bark extract (Cortex betula) from the Betulaceae family. Pharmaceutical Chemistry Journal, 41: 942-949.

- Aguirre, M.C., Delporteand, C. and Backhouse, N., 2006. Topical anti-inflammatory activity of 2 alpha-hydroxy pentacyclic triterpene acids from the leaves of Ugni molinae. Medicinal Chemistry, 14(16): 5673-5677.

- Demirci, B., Paper, D.H., Demirci, F., Hüsnü Can Baser, K. and Franz, G., 2004. Essential oil of Betula pendula Roth buds. Evidence-Based Complementary and Alternative Medicine, 1(3): 301-303.

- Falamas, A., Cınta, S. and Pınzaru, C.A., 2011. Betulin and its natural resource as potential anticancer drug candidate seen by FT-raman and FT-IR spectroscopy. Journal of Raman Spectroscopy, 42(1): 97-107.

- Fan, G., Zhai, Q., Li, X. and Zhan, Y., 2013. Compound of Betula platyphylla cell suspension cultures in response to fungal elicitor. Biotechnology & Biotechnological Equipment, 27(1): 3569-3572.

- Fan, G.Z., Li, X.C., Wang, X.D. and Zhan, Y.G., 2010. Chitosan activates defense responses and triterpenoid production in cell suspension cultures of Betula platyphylla Suk. African Journal of Biotechnology, 9(19): 2816-2820.

- Fletcher, E.K.A., Amoako, T.N.E. and Peter Twumasi, P., 2011. Effect of 2,4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana. African Journal of Biotechnology, 10(46): 9396-9401.

- Gastaldo, P., Caviglia, M,A., Carli, S. and Profumo, P., 1996. Somatic embryogenesis and esculin formation in calli and embryoids from bark explants of Aesculus hippocastanum L. Plant Sciences, 119: 157-162.

- Haroon, A., 2011. Propagation of pecan(Carya Illinoensis)using in vitro techniques. Ph.D thesis, University of the Punjab, Lahore.

- Holonce, L., Ranga, F., Crainic, D., Truta, A. and Socaciu, C., 2012. Evaluation of betulin and betulinic acid content in birch bark from different forestry areas of western carpathians. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(2): 99-105.

- Huang, L., Lee, K.H. and Chen, C.H., 2006. Synthesis and anti-HIV activity of Bi-functional BA derivatives. Bioorganic and Medicinal Chemistry, 14(7): 2279-2289.

- Jafari Hajati, R., Payamnoor, V., Ghasemi Bezdi, K. and Ahmadian Chashmi, N., 2016a. Production of pharmaceutical active ingredients via hairy root induction of Birch (Betula pendula). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24(2): 165-176.

- Jafari hajati, R., Payamnoor, V., Ghasemi Bezdi, K. and Ahmadian Chashmi, N., 2016b. Optimization of callus induction and cell suspension culture of Betula pendula Roth for improved production of betulin, betulinic acid, and antioxidant activity. In Vitro Cellular and developmental Biology-Plant 52(4), 400-407.

- Jäger, S., Trojan, H., Kopp, T., Laszczyk, M.N. and Scheffler, A., 2009. Pentacyclic triterpene distribution in various plants-rich sources for a new group of multi-potent plant extracts. Molecules, 14: 2016-2031.

- Kim, D., Chen, Z.D., Nguyen, V.T., Pezzuto, J.M., Qiu, S.X. and Lu, Z.Z., 1997. A concise semi-synthetic approach to betulinic acid from betulin. Synthetic Communications, 27: 1607-1612.

- Kordalivand, A., 2012. Genetic diversity of Birch based on morphology of leaf and fruit. Thesis submitted in partial fulfillment of a degree of M.Sc. in silviculture and forest ecology, Gorgan University of agricultural sciences and natural resources, 137p.

- Laitinen, M.L., Julkunen-Tiitto, R., Yamaji, K., Heinonen, J. and Rousi, M., 2004. Variation in birch bark secondary chemistry between and within clones: implications for herbivory by hares. OIKOS, 104(2): 316-326.

- Liu, J., Fu, M.L. and Chen, Q.H., 2011. Biotransformation optimization of betulin into betulinic acid production catalysed by cultured Armillaria luteo-virens Sacc ZJUQH100-6 cells. Journal of Applied Microbiology, 110: 90-97.

- Pai, S.R. and Joshi, R.K., 2014. Distribution of betulinic acid in plant kingdom. Plant Science Today, 1(3): 103-107.

- Pisha, E., Chai, H., Lee, I.S., Chagwedera, T.E., Farnsworth, N.R., Cordell, A.C., Beecher, C.W.W., Fong, H.H.S., Kinghorn, A.D., Brown, D.M., Wani, M.C., Wall, M.E., Hieken, T.J., Das Gupta, T.K. and Pezzuto, J.M., 1995. Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nature Medicine, 1: 1046-1051.

- Qi-he, Ch., Ming-liang, F., Jin, L., Hai-feng, Z., Guo-Qing, H. and Hui, R., 2009. Optimizations of ultrasonic-assisted extraction (UAE) of betulin from white birch bark using response surface methodology. Ultrasonics Sonochemistry, 16:
599-604.

- Raal, A., Kanut, M. and Orav, A., 2010. Annual variation of yield and composition of the essential oil of common juniper (Juniperus communis L.) braches from Estonia. Baltic Forestry, 16: 50-56.

- Smith, P.F., Oundele, A., Forrest, A., Wilton, J., Salzwedel, K., Doto, J., Allaway, G.P. and Martin, D.E., 2007. Phase I and II study of the safety, virologic effect, and pharmacokinetics/pharmacodynamics of single-dose 3-O-(3′,3′-dimethylsuccinyl) betulinic acid (bevirimat) against human immunodeficiency virus infection. Antimicrobial Agents and Chemotherapy, 51: 3574-3581.

- Yin, J., Ma, H., Gong, Y., Xiao, J., Jiang, L., Zhan, Y., Li. C., Ren, C. and Yang, Y., 2013. Effect of MeJA and light on the accumulation of betulin and oleanolic acid in the saplings of white birch (Betula platyphylla Suk). American Journal of Plant Sciences, 4: 7-15.

- Zaki, M., Sofi, M.S. and Kaloo, Z.A., 2011. A reproducible protocol for raising clonal plants from leaf segments excised from mature trees of Betula utilis a threatened tree species of Kashmir Himalayas. International Multidisciplinary Research Journal, 1(5): 7-13.

- Zeping, C., Xiao, J., Xianghong, T., Jinglong, J., Fang, L. and Wang, X., 2015. Direct and indirect in vitro plant regeneration and the effect of brassinolide on callus differentiation of Populus euphratica Oliv. South African Journal of Botany, 97: 143-148.

Iranian Journal of Medicinal and Aromatic Plants Research

Effects of explant type on callogenesis and the amount of betulin and betulinic acid produced under in vitro conditions in two birch species (Betula spp.)

References

References

Volume 35, Issue 4 - Serial Number 96
September and October 2019
Pages 577-587

Effects of explant type on callogenesis and the amount of betulin and betulinic acid produced under in vitro conditions in two birch species (Betula spp.)

References

References

Volume 35, Issue 4 - Serial Number 96September and October 2019Pages 577-587

Volume 35, Issue 4 - Serial Number 96
September and October 2019
Pages 577-587