Investigation on the control effects of green copper oxide (CuO) nanoparticles on the tomato gray mold disease caused by Botrytis cinerea

Kazemian, S.; Zarrinnia, V.; Khosroshahli, M.; Hasanzadeh, N.

doi:10.22092/ijmapr.2019.116211.2188

Document Type : Research Paper

Authors

¹ Department of Agricultural Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran

² Department of Plant Protection, Science and Research Branch, Islamic Azad University, Tehran, Iran

³ Department of Plant Protection Science and Research Branch, Islamic Azad University, Tehran, Iran

https://doi.org/10.22092/ijmapr.2019.116211.2188

Abstract

Tomato gray mold disease, caused by Botrytis cinerea, is one of the most important tomato diseases. In recent years, the application of nanoparticles for the control of plant diseases has been given special attention. In this research, the effect of copper oxide nanoparticles biosynthesized by plant extract of eucalyptus was investigated. Three experiments were conducted in order to control the severity of gray mold disease under different growth conditions. In the first experiment, the effects of nanoparticles at concentrations of 100, 200, 400 and 600 ppm were investigated. In the second experiment, two more concentrated extracts i.e. 800 and 1200 were investigated. In the third experiment, the effect of three concentrations of 400, 600 and 800 ppm of copper oxide nanoparticles on the severity of mildew disease on detached leaves was investigated. The results of all three experiments indicated that 1) Copper oxide nanoparticles can control the growth of B. cinerea and gray mildew disease in both in vitro and in vitro conditions. 2) The relationship between the concentration of copper oxide nanoparticles and the inhibitory effect on fungal growth and disease control was invertible and significant at 1% probability level (P≤1%). In fact, the most effective concentrations were obtained at 400 and 600 ppm, 800 and 1200 ppm, and 600 and 800 ppm under in vitro, in vivo,and detached leaf assay, respectively. Accordingly, with increasing concentrations of copper oxide nanoparticles, the rate of growth of the fungus colony decreased and the severity of the disease decreased. Based on these results, the application of green synthesized copper oxide nanoparticles was recommended in controlling of gray mold disease caused by Botrytis cinerea.

Keywords

References

- راحمی، م.، 1384. فیزیولوژی پس از برداشت، مقدمه‌ای بر فیزیولوژی و جابه‌جابجایی میوه، سبزی‌ها و گیاهان زینتی. انتشارات دانشگاه شیراز، 259 صفحه.

- قشم، ر. و کافی، م.، 1378. گوجه‌فرنگی صنعتی، از کاشت تا برداشت. انتشارات جهاد دانشگاهی مشهد، 80 صفحه.

- Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economy and Entomology, 18: 265-267.

- Azwanida, N.N., 2015. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal and Aromatic Plants, 4(3): 1-6.

- Afifipur, Z. and Haghighi, M., 2011. Effect of copper and copper nanoparticles on the germination of pepper. Proceeding of the First National Conference on Modern Agricultural Sciences and Technologies, Iran, Zanjan.

- Bhatia, P., Nanjappa, A., Tissa, S. and David, M., 2004. Tissue culture studies of tomato (Lycopersicon esculentum). Plant Cell Tissue and Organ Culture, 78: 1-21.

- Bondarenko, O., Ivask, A., Käkinen, A. and Kahru, A., 2012. Sub-toxic effects of CuO nanoparticles on bacteria: Kinetics role of Cu ions and possible mechanisms of action. Environmental Pollution, 169: 81-89.

- Chilvers, M.I. and du Toit, L.J., 2006. Detection and identification of Botrytis species associated with neck rot, scape blight, and umbel blight of onion. Plant Health Progress, 7: 1-20, Online.

- Chwalibog, A., Sawosz, E., Hotowy, A., Szeliga, J., Mitura, S., Mitura, K., Grodzik, M., Orlowski, P. and Sokolowska, A., 2010. Visualization of interaction between inorganic nanoparticles and bacteria or fungi. International Journal of Nanomedicine, 6(5): 1085-1094.

- Cioffi, N., Torsi, L., Ditaranto, N., Tantillo, G., Ghibelli, L. and Sabbatini, L., 2005. Copper nanoparticle/polymer composites with antifungal and bacteriostatic properties. Journal of Materials Chemistry, 17: 5255-5262.

- Droby, A. and Lichter, A., 2004. Post-harvest Botrytis infection: etiology, development and management: 349-367. In: Elad, Y., Williamson, B., Tudzynski, P. and Delen, N., (Eds.). Botrytis: Biology, Pathology and Control. Dordrecht, the Netherlands: Kluwer Academic Press, 392p.

- Feng, Q.L., Wu, J., Chen, G.O., Cui, F.Z., Kim, T.N. and Kim, J.O., 2000. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52: 662-668.

- Holister, P., Weener, J.W., Romas Vas, C. and Harper, T., 2003. Nanoparticles: technology white papers 3. Sientific Ltd, 2-11.

- Hua, L., Yong, C., Zhanquan, Z., Boqiang, L., Guozheng, Q. and Shiping, T., 2018. Pathogenic mechanisms and control strategies of Botrytis cinerea causing post-harvest decay in fruits and vegetables. Food Quality and Safety, 2(3): 111-119.

- Ingle, A., Gade, A., Pierrat, S., Sonnichsen, C. and Rai, M., 2008. Mycosynthesis of silver nanoparticles using the fungus Fusarium acuminatum and its activity against some human pathogenic bacteria. Current Nanoscience, 4: 141-144.

- Joerger, R., Klaus, T. and Granqvist, C.G., 2000. Biologically produced silver-carbon composite materials for optically functional thin film coatings. Advanced Materials, 12: 407-409.

- Kim, J.S., Kuk, E., Yu, K.N., Kim, J.H., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y., H. and Hwang, C.Y., 2007. Antimicrobial effects of silver nanoparticles. Nanomedicine, 3: 95-101.

- Kumar, A., Vemula, P.K., Ajayan, P.M. and John, G., 2008. Silver nanoparticle-embedded antimicrobial paints based on vegetable oil. Nature Materials, 7: 236-241.

- Kwak, S.Y., Kim, S.H. and Kim, S.S., 2001. Hybrid organic/inorganic reverse osmosis (RO) membrane for bactericidal antifouling 1 Preparation and characterization of TiO₂ nanoparticle self-assembled aromatic polyamide thin film-composite (TFC) membrane. Environmental Science Technology, 35: 2388-2394.

- Lamsal, K., Kim, S.W. and Jung, J.H., 2011. Effects of Silver nanoparticles against powdery mildews on cucumber and pumpkin. Mycobiology, 39: 26-32.

- Laurance, D.R., Bennett, P.N. and Brown, M.J., 2000. Clinical Pharmacology. Singapore, Longman, 192p.

- Liu, Y., He, L., Mustapha, A., Li, H. and Lin, M., 2009. Antibacterial activities of zinc oxide nanoparticles against Escherichia coli O157:H7. Journal of Applied Microbiology, 107: 1193-1201.

- Mahapatra, S.S. and Khan, M.S., 2007. A neural network approach for assessment quality in technical education: an empirical study. International Journal of Productivity and Quality Management, 2(3): 287-306.

- Mishra, V.K. and Kumar, A., 2009. Impact of metal nanoparticles on the plant growth promoting Rhizobacteria. Digest Journal of Nanomaterials and Biostructures, 3(4): 587-592

- Myresiotis, C.K., Karaoglanidis, G.S. and Tzavella-Klonari, K., 2007. Resistance of Botrytis cinerea isolates from vegetable crops to anilinopyrimidine, phenylpyrrole, hydroxyanilide, benzimidazole, and dicarboximide fungicides. Plant Disease, 91: 407-413.

- Panacek, A., Kolar, M., Vecerova, R., Prucek, R., Soukupova, J., Krystof, V., Hamal, P., Zboril, R. and Kvitek, L., 2009. Antifungal activity of silver nanoparticles against Candida spp. Biometals, 30: 6333-6340.

- Park, H.J., Kim, S.H., Kim, H.J., and Choi, S.H., 2006. A new composition of nanosized silica-silver for control of various plant diseases. Plant Pathology, 22: 295-302.

- Saligkarias, L.D., Gravanis, F.T. and Epton, H.A., 2002. Biological control of Botrytis cinerea on tomato plants by the use of epiphytic yeast Canadia guilliermondii strains101 and us7 and Candida oleophila strain I-182: In vivo studies. Biological Control, 25: 143-150.

- Savithramma, N., Rao, M.L., Rukmini, K. and Devi, P.S., 2011. Antimicrobial activity of silver nanoparticles synthesized by using medicinal plants. International Journal of ChemTech Research, 3(3): 1394-1402.

- Sobhani, A., Abdossi, V., Zarinnia, V. and Oraghi Ardebili, Z., 2014. Evaluation of the antifungal activity of two plant essence on the qualitative and quantitative characteristics of strawberry fruit during storage condition. International Journal of Advanced Biological and Biomedical Research, 2(7): 2238-2246.

- Stoimenov, P.K., 2002. Metal oxide nanoparticles as bactericidal agents. Langmuir, 18(17): 6679-6686.

- Taghavi Fardood, S. and Ramezani, A., 2016. Green synthesis and characterization of copper oxide nanoparticles using coffee powder extract. Journal of Nanostructure, 6(2): 160-164.

- Wani, A.H. and Shah, M.A., 2012. A unique and profound effect of MgO and ZnO nanoparticles on some plant pathogenic fungi. Journal of Applied Pharmaceutical Science, 2(3): 40-44.

- Xu, J.F., Ji, W, Shen, Z.X., Tang, S.H., Ye, X.R., Jia, D.Z. and Xin, X.Q., 1999. Preparation and characterization of CuO nanocrystals. Journal of Solid State Chemistry, 147(2): 516-519.

Iranian Journal of Medicinal and Aromatic Plants Research

Investigation on the control effects of green copper oxide (CuO) nanoparticles on the tomato gray mold disease caused by Botrytis cinerea

References

References

Volume 35, Issue 1 - Serial Number 93
March and April 2019
Pages 54-67

Investigation on the control effects of green copper oxide (CuO) nanoparticles on the tomato gray mold disease caused by Botrytis cinerea

References

References

Volume 35, Issue 1 - Serial Number 93March and April 2019Pages 54-67

Volume 35, Issue 1 - Serial Number 93
March and April 2019
Pages 54-67