Document Type : Research Paper

Authors

• S. Ghasemi ¹
• Z. Habibi ²
• F. Rezaalizadeh Rooshan ³

¹ Department of Chemistry, Ilam Branch, Islamic Azad University, Ilam, Iran

² Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran

³ M.Sc. student, Department of Chemistry, Faculty of Science, Shahid Beheshti University, Tehran, Iran

Abstract

In this study, the chloroform extract of the aerial parts of Eryngium noeanum Boiss.fromUmbelliferae family was investigated for the first time. The aerial parts of this plantwere collected at the flowering stage from Estahban, Fars Province, Iran. The extract was fractionated by the gravity column chromatography on silica gel with a gradient n-hexane, n-hexane-ethyl acetate, ethyl acetate, ethyl acetate–methanol, and methanol as eluent. The similar fractions were combined according to TLC analysis and eight fractions were collected. Two known compounds namely β-stigmasterol (1) and Xanthotoxin (2) were isolated. β-Stigmasterol was extracted from fraction 3 by thin layer chromatography in n-hexane: ethyl acetate (6:1, 30 mg). The sixth fraction was purified using repeated column chromatography and thin layer chromatography in n-hexane: ethyl acetate as the eluent (4:1) to obtain the known coumarin 2 (8 mg). The structure of compounds was determined by their comprehensive spectroscopic analysis including EI-MS, ¹H NMR, ¹³C NMR, H-H COSY, DEPT, HMQC, and comparison with the literature data. 

Keywords

• Umbelliferae
• Eryngium noeanum Boiss
• β-stigmasterol
• xanthotoxin