Biochemical responses to yeast extract and sucrose in black cumin (Nigella sativa L.) cell culture

Anbarestani, S.; Rezazadeh, A.R.; Rezaei, A.

doi:10.22092/ijmapr.2019.116837.2225

Document Type : Research Paper

Authors

¹ M.Sc. graduated of Agriculture Biotechnology, Faculty of Agriculture, Shahed University, Tehran, Iran

² Faculty of Agriculture, Shahed University, Tehran, Iran

https://doi.org/10.22092/ijmapr.2019.116837.2225

Abstract

Black cumin (Nigella sativa L.) is a medicinal plant belonging to the Ranunculaceae family. The seeds of Nigella sativa, also known as black seed, are used in traditional medicine as a natural remedy for several illnesses including asthma, inflammation, diabetes, and hypertension. The cell culture of this plant is important because of its active ingredients and significance in medicine. In this research, the effects of fungal elicitor (zero, 0.5 and 1 mg/L), and sucrose (30, 45 and 60 g/L) on the cell culture of black cumin was investigated as a factorial experiment in a completely randomized design with three replications. The cell growth characteristics and biochemical traits were assayed. The results showed that fungal elicitor and sucrose increased the membrane lipid peroxidation, protein concentration, phenol contents, hydrogen peroxide, and anthocyanin whereas peroxidase activity was significantly decreased compared to control. Generally, sucrose and fungal elicitor increased the growth and activity of the cell and increased the protein production by increasing oxidative stress in cells and increasing the entry of substances into the cell and stimulating metabolism.

Keywords

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Iranian Journal of Medicinal and Aromatic Plants Research

Biochemical responses to yeast extract and sucrose in black cumin (Nigella sativa L.) cell culture

References

References

Volume 34, Issue 6 - Serial Number 92
January and February 2019
Pages 997-1006

Biochemical responses to yeast extract and sucrose in black cumin (Nigella sativa L.) cell culture

References

References

Volume 34, Issue 6 - Serial Number 92January and February 2019Pages 997-1006

Volume 34, Issue 6 - Serial Number 92
January and February 2019
Pages 997-1006