Effects of inoculation of seeds with different strains of bacteria and mycorrhizal fungi on growth, essential oil production, biochemical and physiological characteristics of basil (Ocimum basilicum L.)

Farajzadeh Memari Tabrizi, E.; Roshdi Maleki, M.; Farajzadeh Memari Tabrizi, N.; Ahmadzadeh, V.

doi:10.22092/ijmapr.2018.121269.2287

Document Type : Research Paper

Authors

¹ Department of Agronomy, Malekan Branch, Islamic Azad University, Malekan, Iran

² Department of Microbiology, Malekan branch, Islamic Azad University, Malekan, Iran

³ Young Researchers Club,Tabriz Branch, Islamic Azad University, Tabriz, Iran

https://doi.org/10.22092/ijmapr.2018.121269.2287

Abstract

The aim of this study was to investigate the effects of seed treatment on different strains of bacterial biofertilizers (control, Azosperilium 21, pseudomonas 168, istofyu, her bacillus and a combination of strains) and different strains of mycorrhizal fertilizer (control, Glomus mossae, G. intradices, G. hoei and all three strains of mycorrhiza) on growth, essential oil yield, and physiological characteristics of basil (Ocimum basilicum L.). Experiments were carried out in 2009 as split plot based on randomized complete block design with three replications in the farms of the Malakan Islamic Azad University. The measured traits were crop height, leaf area, chlorophyll index, chlorophyll a content, chlorophyll b content, number of stomata in the leaf surface, number of stomata in the substrate, essential oil content, essential oil yield, dry matter yield, glycol peroxidase, catalase and proline. In this study, mycorrhizal fertilizer treatments had a positive effect on the growth and yield of basil essential oil. In addition to the improvement of growth indices, physiological parameters including chlorophyll a and b contents, number of stomata and antioxidant content increased as a result of application of manure. The essential oil yield increased by 16.1%, 11.9% and 17.9%, respectively, due to the application of Azospirilum 21, application of bacteria, and the combined application of bacterial biofertilizer strains. This increase was mainly due to the increase in the percentage of basil essential oil. Contrary to growth characteristics, physiological characteristics such as chlorophyll a and b content and antioxidant content were positively affected by the use of bacterial biofertilizer. According to the results of this study, the application of Azospirilium 21, application of the bacteria, and the combined application of bacterial biofertilizer strains along with each of the mycorrhizal fungi strains will increase the qualitative and quantitative properties of basil essential oil.

Keywords

References

- Abdallah, M.M., Abd El-Monem, A.A., Hassanein, R.A. and El-Bassiouny, H.M.S., 2013. Response of sunflower plant to the application of certain vitamins and arbuscular mycorrhiza under different water regimes. Australian Journal of Basic and Applied Sciences, 7(2): 915-932.

- Aebi, H., 1984. Catalase in Vitro. 105: 121-126, In: Packer, L., (Ed.), Methods in Enzymology, Academic Press, San Diego, 600p.

- Afzal, A. and Bano, A., 2008. Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticum aestivum). International Journal of Agriculture and Biology, 10: 85-88.

- Akhtar, M.S. and Siddiqui, Z.A., 2009. Effects of phosphate solubilizing microorganisms and Rhizobium sp. on the growth, nodulation, yield and root-rot disease complex of chickpea under field condition. African Journal of Biotechnology, 8(15): 3489-3496.

- Aly, M.S., EL-Shahat, A.N., Naguib, N.Y., Ahl, H.A.S., Zakaria, A.M. and Abou Dahab, M.A., 2015. Effect of nitrogen and/or bio-fertilizer on the yield, total flavonoids, carbohydrate contents, essential oil quantity and constituents of dill plants. Middle East Journal of Agriculture, 4: 291-296.

- Banchio, E., Xie, X., Zhang, H. and Pare, P.W., 2009. Soil bacteria elevate essential oil accumulation and emissions in sweet basil. Journal of Agricultural and Food Chemistry, 57: 653-657.

- Bargali, K. and Bargali, S.S., 2009. Effect of phosphorus nutrition on growth and mycorrhizal dependency of Coriaria nepalensis seedlings. Nature and Science, 7(6): 19-24.

- Boiero, L., Perrig, D., Masciarelli, O., Penna, C., Cassán, F. and Luna, V., 2007. Phytohormone production by three strains of Bradyrhizobium japonicum and possible physiological and technological implications. Applied Microbiology and Biotechnology, 74: 874-880.

- Bonfante, P. and Genre, A., 2008. Plants and arbuscular mycorrhizal fungi: an evolutionary developmental perspective. Trends in Plant Sciences, 13: 492-498.

- Emami, H., Saeidnia, M., Hatamzadeh, A., Bakhshi, D. and Ghorbani, E., 2011. The effect of gibberellic acid and benzyladenine in growth and flowering of lily (Lilium longiflorum). Advances in Environmental Biology, 5(7): 1606-1611.

- Farber, M., Attia, Z. and Weiss, D., 2016. Cytokinin activity increases stomatal density and transpiration rate in tomato. Journal of Experimental Botany, 67: 6351-636.

- Gholami, A., Amin Alavi, S., Moezi, A. and Salimpour, S., 2013. The effect of mycorrhiza fungi (VAM) on phosphorus absorption by corn (Zea Mays L.) at northern khouzestan, Iran. Scientific Journal of Agronomy and Plant Breeding, 1(2):
32-36.

- Hedayati Mahdi Abadi, B., Ganjali, H.R. and Mobasser, H.R., 2015. Effect of mycorrhiza and phosphorus fertilizer on some characteristics of black cumin. Biological Forum-An International Journal, 7(1): 1115-1120.

- Heidari, M., Mousavinik, S.M. and Golpayegani, A., 2011. Plant growth promoting rhizobacteria (pgpr) effect on hysiological parameters and mineral uptake in basil (Ociumum basilicm L.) under water stress. ARPN Journal of Agricultural and Biological Science, 6: 78-91.

- Hellal, F.A., Mahfouz, S.A. and Hassan, F.A.S., 2011. Partial substitution of mineral nitrogen fertilizer by bio-fertilizer on (Anethum graveolens L.) plant. Agriculture and Biology Journal of North America, 2(4): 652-660.

- Jafari Ghoushchi, M., Abbaszadeh, B. and Oraei, M., 2015. Effects of chemical and biological fertilizers on growth, yield and essential oil of Salvia officinalis. Journal of Medicinal Plants and By-Products, 4: 31-37.

- Javadi, H., Seyed, M., Hesamzadeh, H. and Majnun, B., 2013. Comparison of karyotypic triats of Thymus pecies in Iran. Annals of Biological Research, 4(1): 199-208.

- Jha, S.K. and Kumar, N., 2011. Potential of mycorrhizal fungi in ecosystem: a review. International Journal of Research in Botany, 1(1): 1-7.

- Khair-ul-Bariyah, S., Ahmed, D. and Ikram, M., 2012. Ocimum basilicum: a review on phytochemical and pharmacological studies. Journal of the Chemical Society of Pakistan, 2(2): 78-85.

- Khalil, S.E. and Yousef, R.M.M., 2014. Interaction effects of different soil moisture levels, arbuscular mycorrhizal fungi and three phosphate levels on: I- growth, yield and photosynthetic activity of garden cress (Lepidium sativum L.) plant. International Journal of Advanced Research, 2: 723-737.

- Kumar, A., Mangla, C., Aggarwal, A. and Srivastava, V., 2014. Rhizospheric effect of endophytic mycorrhiza and trichoderma viride on physiological parameters of Mentha Spicata linn. Asian Journal of Advanced Basic Sciences, 2(1): 99-104.

- Mahfouz, S.A. and Sharaf-Eldin, M.A., 2007. Effect of mineral vs. biofertilizer on growth, yield, and essential oil content of fennel (Foeniculum vulgare Mill.). International Agrophysics, 21: 361-366.

- Mathivanan, S., Chidambaram, A.L.A., Sundaramoorthy, P., Baskaran, L. and Kalaikandhan, R., 2014. The effect of plant growth promoting rhizobacteria on groundnut (Arachis hypogaea L.) seed germination and biochemical constituents. International Journal of Current Research and Academic Review, 2: 187-194.

- Mohammadi, K. and Sohrabi, Y., 2012. Bacterial biofertilizers for sustainable rop production: A review. Journal of Agricultural and Biological Science, 7: 307-316.

- Ngoc, S.T.T., Ngoc, D.C.M. and Giang, T.T., 2006. Effect of Bradyrhizobia and phosphate solubilizing bacteria application on soybean in rotational system in the Mekong delta. Journal of Omonrice, 14: 48-57.

- Robinson, J., Nithya, K., Ramya, R., Karthikbalan, B. and Kripa, K., 2014. Effect of vesicular arbuscular mycorrhiza Glomus fasiculatum on the growth and physiological response in Sesamum indicum L. International Letters of Natural Sciences, 23: 47-62.

- Roshanpour, N., Taghi Darzi, M. and Haj Seyed Hadi, M., 2014. Effects of plant growth promoter bacteria on biomass and yield of basil (Ocimum basilicum L.). International Journal of Advanced Biological and Biomedical Research, 2: 2077-2085.

- Sivagurunathan, P., Sathiyamoorthy, M. and Sivasubramani, K., 2014. Effect of mycorrhizal fungi on growth of Zea mays L. Plants. International Journal of Advanced Research in Biological Sciences, 1(1): 137-148.

- Srinivasa Murthy, K.M. and Narayanappa, M., 2013. Influence of vesicular arbuscular mycorrhizae (VAM) on growth of Ruta graveolens-a medicinal plant. International Journal of Science and Research, 4(5): 897-900.

- Srinivasan, R. and Govindasamy, C., 2014. Influence of native arbuscular mycorrhizal fungi on growth, nutrition and phytochemical constituents of Catharanthus roseus (L.) G. Don. Journal of Coastal Life Medicine, 2(1): 31-37.

- Tao, G., Tian, S., Cai, M. and Xie, G., 2008. Phosphate solubilizing and mineralizing abilities of bacteria isolated from soils. Pedosphere, 18: 515-523.

- Upadhyaya, A., Sankhla, D., Davis, T.D., Sankhla, N. and Smith, B.N., 1985. Effect of paclobutrazol on the activities of some enzymes ofactivated oxygen metabolism and lipid peroxidation in senescingsoybean leaves. Journal of Plant Physiology, 121: 453-461.

- Weisany, W., Rahimzadeh, S. and Sohrabi, Y., 2012. Effect of biofertilizers on morphological, physiological characteristic and essential oil content in basil (Ocimum basilicum L.). Iranian Journal of Medicinal and Aromatic Plants, 28: 73-87.

- Yadav, A. and Aggarwal, A., 2015. The associative effect of arbuscular mycorrhizae with Trichoderma viride and Pseudomonas fluorescens in promoting growth, nutrient uptake and yield of Arachis hypogaea L. New York Science Journal, 8(1): 101-108.

- Zolfaghari, M., Nazeri, V., Sefidkon, F. and Rejali, F., 2013. Effects Effect of arbuscular mycorrhizal fungi on plant growth and essential oil content and composition of Ocimum basilicum L. Iranian Journal of Plant Physiology, 3(2): 643-650.

Iranian Journal of Medicinal and Aromatic Plants Research

Effects of inoculation of seeds with different strains of bacteria and mycorrhizal fungi on growth, essential oil production, biochemical and physiological characteristics of basil (Ocimum basilicum L.)

References

References

Volume 34, Issue 5 - Serial Number 91
November and December 2018
Pages 805-819

Effects of inoculation of seeds with different strains of bacteria and mycorrhizal fungi on growth, essential oil production, biochemical and physiological characteristics of basil (Ocimum basilicum L.)

References

References

Volume 34, Issue 5 - Serial Number 91November and December 2018Pages 805-819

Volume 34, Issue 5 - Serial Number 91
November and December 2018
Pages 805-819