Antioxidant effects of sage (Salvia officinalis L.) extract and chitosan-treated sage on liver factors and lipid profiles in male Wistar rats

Saeidian, Sh.; Valizadeh, Y.; Amaki, Z.

doi:10.22092/ijmapr.2018.116793.2223

Document Type : Research Paper

Authors

¹ Department of Biochemistry, Payame Noor University, Tehran, Iran

² Ph.D. Student of Biology, Tehran University, Tehran, Iran

³ M.Sc. student of Biochemistry, Payame Noor University, Tehran, Iran

https://doi.org/10.22092/ijmapr.2018.116793.2223

Abstract

Due to the medicinal properties, the root of sage (Salvia officinalis L.) has been used from a very distant past. In the present study, the homogenate of sage and the sage treated with chitosan was extracted after drying using 96% ethanol. Then, ethanol solvent was removed from the extract using rotary equipment. To do this research, 60 adult male white Wistar rats were used. The rats were divided into six groups of 10. The oral extract of sage and the sage treated with chitosan was used at a dose of 200 mg/kg. In addition, a dose of 50 mg/kg of isoniazid was used as gavage to induce oxidative effect. The DPPH method was also used with the help of 1,2-diphenyl 1-picyril hydrazine to compare the antioxidant effects of sage and the sage treated with chitosan. The use of alcoholic extract of Salvia sp. led to reduced triglyceride, cholesterol, LDL and ALP as well as increased HDL levels. In addition, the extract of sage treated with chitosan led to decreased LDL, ALT and increased HDL levels. Therefore, according to the results, it was concluded that the beneficial effects of the factors evaluated were significant. Also, the DPPH test results showed that antioxidant effects of alcoholic extract of the sage treated with chitosan was much higher and more effective than sage, indicating the positive effect of chitosan treatment on the amount of sage antioxidants. The results of this study confirmed the undesirable effects of isoniazide on the cells of body.

Keywords

References

- Ahmadi, R., Balali, Sh., Tavakoli, P., Mafi, M. and Haji, Gh., 2013. The effect of hydroalc oholic leaf extract of Salvia officinalis on serum levels of FSH, LH, testosterone and testicular tissue in rats. Journal of Kashan University of Medical Sciences, 17(3): 225-231.

- Arabi, S., Arshami, J. and Haghparast, A.R., 2014. Effects of Salvia officinalis L. extract on biochemical blood parameters in male rats. Journal of Zanjan University of Medical Sciences, 22(94): 34-43.

- Carta, C., Moretti, M.D.L. and Peana, A.T., 1996. Activity of oil Salvia officinalis against of Botrytis cinerea. Journal of Essential Oil Research, 8(4): 399-404.

- Chien, P.J., Sheu, F. and Lin, H.R., 2007. Coating citrus (Murcott tangor) fruit with low molecular weight chitosan increases postharvest quality and shelf life. Food Chemistry, 100: 1160-1164.

- Cook, N.C. and Saman, S., 1996. Flavonoids chemistry, metabolism, cardio protective effects, dietary sources. The Journal of Nutritional Biochemistry, 7(2): 66-76.

- Fleming, T., 1998. PDR for Herbal Medicines. Montvale, Medical Economics Company, 1244p.

- Jimenez-Escrig, A., Jimenez-Jimenez, I., Sanchez-Moreno, C. and Saura-Calixto, F., 2000. Evaluation of free radical scavenging of dietary carotenoids by the stable radical 2,2 diphenyl-1picrylhydrazy1. Journal of the Science of Food and Agriculture, 80(11): 1686-1690.

- Ghasemloo, E., Rahnema, M. and Bigdeli, MR., 2015. The effect of hydroalcoholic extract of Salvia officinalis on brain edema and neurologic deficits in rat stroke model. Journal of Fasa University of Medical Sciences. 5(3): 378-386.

- Khosravi, M., Khakpor, Sh., Mirzaei, M. and Najjari, M., 2010. Effect of homogenate of Salvia officinalis on LDL, HDL and triglyceride at male rat. Journal of Developmental Biology, 3(10): 15-24.

- Khosravi, M., Khakpor, Sh., Tajadod, Gh. and Takzobani belasi, F., 2013. Effect of Saliva officinalis hydroalcoholic extract on liver enzymes in male rat. Medical Science Journal of Islamic Azad University. 23(2): 113-119.

- Kianbakht, S., Rezazadeh, Sh., Razaghi Azar, M., Khamseh, A. and Baradaran, H.R., 2012. Study on effects of Salvia officinalis L. leaf extract on blood lipid levels of hyperlipidemic patients with type 2 diabetes mellitus. Plan of Iranian Instituates of Medicinal Plants.

- Lima, C.F., Andrade, P.B., Seabra, R.M., Fernandes-Ferreira, M. and Pereira-Wilson, C., 2005. The drinking of a Salvia officinalis infusion improves liver antioxidant status in mice and rats. Journal of Ethnopharmacology, 97(2): 383-389.

- Mohammadi Zeidi, I., Akaberi, A. and Pakpour, A.H., 2012. Factors associated with herbal medicine use among women in Qazvin city: application of theory of planned behavior. Journal of North Khorasan University of Medical Sciences, 4: 103-114.

- Nagy, G., Gunther, G., Mathe, I., Blunden, G., Yang, M. and Crabb, T.A., 1999. Diterpenoids from Salvia glutinosa, S. austriaca, S. tomentosa and S. verticillata roots. Phytochemistry Journal, 52(6): 1105-1109.

- Nan, J.X., Park, E.J., Kanq, H.C., Park, P.H., Kim, J.Y. and Sohn, D.H., 2001. Anti-fibrotic effects of a hot-water extract from Salvia miltiorrhiza roots on liver fibrosis induced by biliary obstruction in rats. Journal of Pharmacy and Pharmacology, 53(2): 197-204.

- Nickavar, B., Mojab, F. and Asgarpanah, J., 2005. Volatile composition of the essential oil of Salvia hypoleuca Benth. International Journal of Aromatherapy, 15: 51-53.

- Orhan, I., Kartal, M., Naz, Q., Ejaz, A., Yilmaz, G., Kan, Y., Konuklugi, B., Şener, B. and Choudhary, M.I., 2006. Antioxidant and anticholinesterase evaluation of selected Turkish Salvia species. Food Chemistry, 103(4): 1247-1254.

- Panda, S. and Kar, A., 1998. Dual role of betel leaf extract on thyroid function in male mice. Pharmacological Research, 38(6): 493-496.

- Pino, J.A., Estrarron, M. and Fuentes, V., 1999. Essential oil of sage (Salvia officinalis L.) grown in Cuba. Journal of Essential Oil Research, 9(2): 221-222.

- Ranjbar, A., Ghasmeinezhad, S., Zamani, H., Malekirad, A.A., Baiaty, B., Mohammadirad, A. and Abdollahi, M., 2006. Antioxidative stress potential of Cinnamomum zeylancium in human: a cross-sectional clinical study. Therapy, 3: 15-111.

- Ranjbar, A., Khorami, S., Safarabadi, M., Shahmoradi, A., Malekirad, A.A., Vakilian, K., Mandegary, A. and Abdollahi, M., 2006. Antioxidant activity of Iranian Echium amoenum Fisch & C.A.Mey flower decoction in humans. Evidence-Based Complementary and Alternative Medicine, 3(4): 469–473.

Iranian Journal of Medicinal and Aromatic Plants Research

Antioxidant effects of sage (Salvia officinalis L.) extract and chitosan-treated sage on liver factors and lipid profiles in male Wistar rats

References

References

Volume 34, Issue 3 - Serial Number 89
July and August 2018
Pages 466-477

Antioxidant effects of sage (Salvia officinalis L.) extract and chitosan-treated sage on liver factors and lipid profiles in male Wistar rats

References

References

Volume 34, Issue 3 - Serial Number 89July and August 2018Pages 466-477

Volume 34, Issue 3 - Serial Number 89
July and August 2018
Pages 466-477