In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

Jahad-daneshgahi of Tehran University, Department of Applied Microbiology

Abstract

Bunium persicum is an umbellifer with dormant seeds that is not cultivated in Iran. Since, the seeds of Bunium persicum have pharmacotical effects, termination of dormancy in these seeds is important. The study of phenology of Bunium persicum showed that the seeds germinated in spiring and required exposure to cold temperature before germination. Also ,previous studies showed that some concentration of plant growth regulators overcome seed dormancy in this plant . In this research ,effect of cold stratification with or whitout above plant growth regulators in  dormancy breaking of these seeds was investigated. In this work effect of stratification  on dormancy breaking and induction of germination and combined effect(s) of hormonal treatments and stratification on breakage of seed dormancy were determined . To identify effect of stratification on seed dormancy ,the seeds stored for 0,1, 2 ,3 , 4, 6 , 8 or 10 weeks at 4°C in refrigerator and then transfer to favorable conditions for germination,and  to study of combination effects of hormonal treatments and stratification , the seeds that exposed to 4°C for 4 weeks were subjected to kinetin, or benzyladenine(0 or 10 –5 mM ). Results showed that stratification is require for dormancy breaking in Bunium persicum seeds and increasing the duration of stratification increased germination . Also, combining stratification and benzyladenine promoted germination .

Keywords

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