Document Type : Research Paper
Authors
1 Department of Pharmacology, Medical Science University of Mashhad
2 Department of Chemistry, Ferdowsi University of Mashhad
3 PhD student of Ferdowsi University
Abstract
In this research, the extraction and separation of sedative compounds of Rosa damascena Mill. (from Rosaceae family) was studied. The minimum dosage of sedative effect was the basis of physiological tests (on Balb-C mouse) which has done in MashhadUniversity of medical sciences. Several extractions were done by ethanol, chloroform and water, among which the extracted syrup from ethanol showed the best effect (500mg/kg). This syrup showed five main spots on TLC (thin layer chromatography), from which the desired compound was separated by column chromatography. The physiological tests of the pure compound from column chromatography showed that the minimum effective dosage was 15mg/kg. The chemical structure of separated compounds was also studied by Mass, IR and NMR spectroscopy.
Keywords
)