Document Type : Research Paper

Authors

• H. Jaffari ¹
• N. Gheibi ²
• E. Abbsai ³
• S.R. Miri ⁴
• M. Khalili ⁵
• M. Shahidi ⁶
• S. Yadegari ⁵
• S.M. Nabavi ⁷

¹ Dept. of Pharmacology, Qazvin medical University

² Dept. of Biophysics, Qazvin Medical University

³ Dept. of physiology, Islamic Azad University

⁴ Physician

⁵ Dept. of physiology, Shahed University

⁶ Dept. of Hematology, Iran Medical Science University

⁷ Dept. of Neurology, Shahed University

Abstract

Drug addiction is destructive misfortune and one of the most important problems in the world. Using the medical plants in medicinal history has valuable information and experiments in this field. According to the researches, Trachyspermum copticum L. (T.C) is effective in reducing of morphine’s withdrawal syndrome, so, in this study we investigate the effects of T.C microinjection on nucleus reticularis paragigantocellularis (PGi) on morphine’s withdrawal syndrome sings (MWSS). After preparing the T.C fruit from height of the mountain in Khuzestan, aqueous extract of different concentration (Diluted 10, 100, 1000 times) was prepared by soxhelet display. Thirty-two male rats (Sprague Dawley with average weigh 250-300gr) were divided to 4 groups, randomized which dependent by intraperitoneum (I.P) injection with morphine. After cumulating the PGI with using the set of steriotaxi, microinjection of different doses of T.C in PGI was done and naloxan (5mg/kg) was injected I.P to groups. Withdrawal syndrome signs (weight loss, jumping, climbing, and abdominal pain) were observed in 4 groups. Data evaluation and analysis was done by LSD and ANOVA. The results showed T.C was significantly effective in reduction of MNSS such as (jumping, climbing, abdominal pain) compared with control group. So, T.C is probably sensitive on specific receptors in PGI.

Keywords

• Trachyspermum copticum L
• Paragigantocellularis
• withdrawal syndrome
• rat