Extraction and Determination of Tropan Alkaloids, Hyoscyamine and Scopolamine, from Different Parts of Hyoscyamus pusillus L. in Different Stages of Plant Growth

Dialmaghani, K.; Kharvari-Nejad, R.; Fahimi, H.; Hekmat- Shoar, H.

doi:10.22092/ijmapr.2006.114993

Document Type : Research Paper

Authors

¹ Islamic Azad University, Science and Researches Branch

² Tabriz University

https://doi.org/10.22092/ijmapr.2006.114993

Abstract

Hyoscyamus pusillus from Solanaceae family is a medicinal plant producing tropane alkaloids. For investigation of tropane alkaloids levels in H. pusillus, plant materials were collected during three different growth stages, vegetative stage, flowering stage and fruiting stage, from two regions of Azarbayjan, Marand and Tabriz. After extraction and purification of alkaloids from different parts of plants (root, stem, leaves, flowering tops and seeds), were assayed by HPLC. Furthermore, in two regions effects of environmental factors upon tropane alkaloid levels were investigated. The results showed that hyoscyamine and scopolamine content varied in three stages and in different parts of plants. The highest level of hyoscyamine in two regions was observed in leaves at flowering stage, whereas there was lowest level in stems at vegetative stage. Hyoscyamine was dominant alkaloid, with except of seeds, in all organs. Furthermore, the results showed that hyoscyamine and scopolamine content of plants in Marand region at all growth stages was higher than that of plants in Tabriz region. The results of investigation of effects environmental factors on alkaloid levels showed that some of this factors influenced production of tropane alkaloids. For example, as altitude up, alkaloid levels are raised. Furthermore, increased nitrogen and phosphorus concentrations in soil caused increasing alkaloid levels in plants. Whereas, in contrast, reduced potassium in soil caused increasing alkaloid levels. As plant grows alkaloid levels increase. This can be results from it when plant mature, high nitrogen enters hyoscyamine (and as a result scopolamine) structure.

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Extraction and Determination of Tropan Alkaloids, Hyoscyamine and Scopolamine, from Different Parts of Hyoscyamus pusillus L. in Different Stages of Plant Growth

References

References

Volume 22, Issue 1 - Serial Number 31
April 2006
Pages 1-10

Extraction and Determination of Tropan Alkaloids, Hyoscyamine and Scopolamine, from Different Parts of Hyoscyamus pusillus L. in Different Stages of Plant Growth

References

References

Volume 22, Issue 1 - Serial Number 31April 2006Pages 1-10

Volume 22, Issue 1 - Serial Number 31
April 2006
Pages 1-10