Document Type : Research Paper

Authors

• E. Aghili ¹
• K. Shahanipoor ²
• L. Amjad ³

¹ M.Sc. student, Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

² Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

³ Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Abstract

The genus Anthemis is the largest genera of the Asteraceae family. Anthemis gayana Boiss. alters the activity of liver enzymes and reduces liver enzymes and oxidative stress. Paracetamol (acetaminophen) is widely used as an antipyretic and analgesic drug, which at high levels leads to an increase in liver transaminase activity in humans and animals. The present study was aimed to determine the protective effect of Anthemis gayana methanolic extract on liver function and oxidative stress in Wistar rats treated with paracetamol. In this experiment, 40 male wistar rats were divided into eight groups of five. Animals in the first group (control) received normal saline. The second group (placebo) received paracetamol (300mg/kg). The third, fourth, and fifth group received Anthemis gayana methanolic extract (100, 200, 300mg/kg) and the sixth, seventh and eighth group received Anthemis gayana methanolic extract (100, 200, 300mg/kg) and paracetamol (300mg/kg) intraperitoneally for 30 days. AST, ALT, ALP, MDA were measured 48 hours after the last injection. The results showed a significant increase AST, ALT, ALP activity in serum in the group treated with paracetamol as compared with control group (P<0.05). The methanolic extract of Anthemis gayana showed protective effect on liver and this effect was proved by a significant decrease of ALT and MDA in serum in the group received the methanolic extract of Anthemis gayana (P<0.05). Our results clearly showed the protective effect of Anthemis gayana methanolic extract on liver enzymes and oxidative stress.

Keywords

• Anthemis gayana Boiss
• Liver enzymes
• methanolic extract
• acetaminophen