Iranian Journal of Medicinal and Aromatic Plants Research

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Investigation of genetic and phytochemical diversity of Teucrium Polium L. germplasm in Kerman province by RAPD molecular marker and GC/MS method

Document Type : Research Paper

Authors

1 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

2 Payam Noor University, Tehran, Iran

Abstract

Teucrium polium L. is an herbaceous, perennial and hyperbranched plant. Teucrium polium grows in different parts of Europe and the Middle East, such as Iran. In order to determination of genetic diversity of Teucrium Polium gemlplasm in Kerman province, 15 genotypes were collected. The DNA was extracted using CTAB method. Fifteen RAPD primers were used for PCR. The electrophoresis results were analyzed using NTSYS software applying UPGMA method with Dice coefficient. To assess the phyto-chemical diversity of Teucrium polium germplasm, the essential oil of seven genotypes of Teucrium Polium was prepared by hydro distillation. The identification and analysis of essential oil composition was done by GC/MS with calculation and study of retention indices and mass spectrometry. Cluster analysis was done for the results of GC/MS by SPSS12 software. After electrophoresis of PCR products, 182 bands were found in the range of 230 to 2300 bp. A number of 169 bands (93%) were polymorphic. The genetic similarity of the genotypes was 0.37 to 0.72. Based on cluster analysis of molecular data, the collected samples were divided into four groups. The essential oil constituents in Kohbanan, Baghin, Shahrbabak, Anbarabad, Ravar and Kerman genotypes were identified. The samples were classified into three groups by cluster analysis of phytochemical data.

Keywords

References
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Iranian Journal of Medicinal and Aromatic Plants Research
Volume 33, Issue 4 - Serial Number 84
October 2017
Pages 673-681
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