Effects of basal media and some physical parameters on micropropagation of stevia (Stevia rebaudiana Bertoni)

Ebrahimi, M.; Mokhtari, A.; Amirian, R.

doi:10.22092/ijmapr.2017.107061.1828

Document Type : Research Paper

Authors

¹ Department of Plant Tissue Culture, Agricultural Biotechnology Research Institute of Iran-Central Branch- Isfahan, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran,

² Department of Plant Tissue Culture, Agricultural Biotechnology Research Institute of Iran-Central Branch- Isfahan, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

³ Department of Genomics, Agricultural Biotechnology Research Institute of Iran-Central Branch- Isfahan, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

https://doi.org/10.22092/ijmapr.2017.107061.1828

Abstract

Stevia (Stevia rebaudiana Bertoni) is a cross-pollinated plant whose seed vigor is poor. Therefore, the vegetative propagation approaches like in vitro culture techniques could be the best choice for mass and true-to-type production of stevia plantlets. This study was aimed to evaluate the effects of basal media (MS, B5 and LS) and physical parameters such as light (55 and 110 μmol m^-2 s^-1) and temperature (22, 25 and 27ºC) on in vitro culture of stevia. The nodal segments were cultured on basal media containing various combination of BAP and IAA or IBA. The ANOVA showed significant differences (P≤0.05) among treatments on micropropagation of stevia. In another experiment, the effect of mT (0.5, 1, 1.5 and 2 mg/l) was also compared with the best treatment from the first experiment. It was found that mT at 1 mg/l was the best concentration. The genetic stability of micropropagated plantlets relative to the mother plant was achieved by AFLP marker using 12 selected primers. In this study, 231 reproducible bands around 50-500bp were scored, indicating similarity of in vitro plantlets with the donor.

Keywords

References

- Ahmed, M.B., Salahin, M., Karim, R., Razvy, M.A., Hannan, M.M., Sultana, R., Hossain, M. and Islam, R., 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Scientific Research, 2: 121-125.

- Akita, M., Shigeoka, T., Koizumi, Y. and Kawamura, M., 1994. Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Reports, 13: 180-183.

- Amoo, S.O., Finnie, J.F. and Van Staden, J., 2011. The role of meta-topolins in alleviating micropropagation problems. Plant Growth Regulation, 63: 197-206.

- Anbazhagan, M., Kalpana, M., Rajendran, R., Natarajan, V. and Dhanave, D., 2010. In vitro production of Stevia rebaudiana Bertoni. Emirates Journal of Food and Agriculture, 22(3): 216-222.

- Bairu, M.W., Stirk, W.A., Dolezˇal, K. and Van Staden, J., 2008. The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tissue and Organ Culture, 95: 373-379.

- Bhojwani, S.S. and Dantu, P.K., 2013. Plant Tissue Culture: An Introductory Text. Springer India, 318p.

- Czechowiak, C., Dubois, J. and Vasseur, J., 1984. Culture in vitro du Stevia rebaudiana Bertoni C.R. Seances. Comptes Rendus de l'Académie des Sciences-Series III-Sciences de la Vie, 298: 173-176.

- El-Zaidy, M.Z., Zayed, M.E., Alharbi, S., Doaigey, A., Al Sahli, A.A. and Ammar, R.A.A., 2014. Micropropagation of seven Stevia rebaudiana Bert. genotypes via adult leaf explants. Journal of Pure and Applied Microbiology, 8(2): 1289-1298.

- Ferreira, C.M. and Walter, H., 1988. Production, maintenance and plant regeneration from cell suspension culture of Stevia rebaudiana (Bert.) Bertoni. Plant Cell Reports, 7: 123-126.

- Filho, B., João, C. and Kazumi, H., 1997. Embryogenic callus formation and histological studies from Stevia rebaudiana (Bert.) Bertoni floret explant. Revista Brasileira de Fisiologia Vegetal, 9(3): 185-188.

- Gabryszewska, E. 2016. Efect of diferent sucrose and nitrogen salt levels in the medium and temperature on in vitro propagation of Helleborus niger L. Acta Agrobot, 68(2):161-171.

- Goettemoeller, J. and Ching, A., 1999. Seed germination in Stevia rebaudiana: 510-511. In: Janick, J., (Ed.). Perspectives on New Crops and New Uses. ASHS Press, 528p.

- Hanson, J.R. and De Oliveira, B.H., 1993. Stevioside and related sweet diterpenoid glycosides. Natural Product Reports, 10: 301-309.

- Handro, W., Ferreira, C.M. and Floh, E.I.S., 1993. Chromosomal variability and growth rate in cell suspension cultures of Stevia rebaudiana (Bert.) Bertoni. Plant Science, 93: 169-176.

- Hossain, M.A., Kabir, A.H.M.S., Japhan, T.A. and Hasan, M.N., 2008. Micropropagation of Stevia rebaudiana. International Journal of Sustainable Crop Production, 3: 1-9.

- Ibrahim, A.I., Nasr, M.I., Mohammded, B.R. and El-Zefzaﬁ, M.M., 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Technology, 10: 254-259.

- Javad, S., Naz, S., Ilyas, S., Ali, A., Aslam, F. and Munir, N., 2012. Study of the effect of physical state of medium and different concentrations of sucrose, ferric ethylenediamine-tetraacetic acid (FeEDTA) and CuSO₄ in enhancing the micropropagation system of Stevia rebaudiana. Journal of Medicinal Plant Research, 6: 1800-1805.

- Kőszeghi, S., Bereczki, C., Balog, A. and Benedek, K., 2014. Comparing the effects of benzyladenine and meta-topolin on sweet basil (Ocimum basilicum) micropropagation. Notulae Scientia Biologicae, 6(4): 422-427.

- Kumar, A., Jena, A., Panda, M.K. and Nayak S., 2009. Effect of phytoregulators on in vitro mass propagation of Stevia rebaudiana Bertoni. International Journal of Integrative Biology, 8(1): 56-59.

- Kumar, S. and Singh, N., 2009. In vitro propagation of Stevia rebaudiana Bertoni: An important medicinal sweet herb. Environment Ecology, 27: 459-464.

- Latha, S. and Usha, M., 2003. In vitro culture studies on Stevia rebaudiana. In Vitro Cellular & Developmental Biology-Plant, 39: 520-523.

- Maiti, R.K. and Purohit, S.S., 2008. Stevia: A Miracle Plant for Human Health. Agrobios (India) Jodhpur, India, 190p.

- Mehrizadeh, V. and Baghban, B.K., 2014. Rooting effect of active charcoal for high scale micropropagation of stevia (Stevia rebaudiana Bertoni). International Journal of Biosciences, 4(11): 234-239.

- Mirniam, A., Roshandel, P., Otroshi, M. and Ebrahimi, M., 2013. A novel protocol for Stevia rebaudiana Bert regeneration. Journal of Advanced Laboratory Research in Biology, 4(1): 15-22.

- Mitra, A. and Pal, A., 2007. In vitro regeneration of Stevia rebaudiana (Bert.) from nodal explants. Journal of Plant Biochemistry and Biotechnology, 16: 59-62.

- Morini, S., Fiaschi, G., Andolfi, L. and Macchia, M., 2003. In vitro propagation of Stevia rebaudiana Bertoni: results with different genotypes. Agricoltura Mediterranea, 133(2): 117-123.

- Mubarak, M., 2008. Micropropagation of Stevia rebaudiana In vitro. Meeting the Challenges of Sugar Crops & Integrated Industries in Developing Countries, Al Arish, Egypt, 11-14 September: 293-298.

- Nakamura, S. and Tamura, Y., 1985. Variation in the main glycosides of Stevia (Stevia rebaudiana Bertoni). Japanese Journal of Tropical Agriculture, 29: 109-116.

- Ramakrishna, A. and Ravishankar, G.A., 2013. Diterpene sweeteners (Steviosides): 3194-3203. In: Ramawat, K.G. and Me´rillon, J.M., (Eds.). Natural Products, Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes, Springer, 4245p.

- Singh, N., Yadav, K., Kumari S. and Renu 2011. Metabolic changes during differentiation in callus cultures of Stevia rebaudiana (Bertoni). Journal of Phytology, 3(3): 63-67.

- Singh, P., Dwivedi, P. and Atri, N., 2014. In vitro shoot multiplication of Stevia and assessment of stevioside content and genetic fidelity of the regenerants. Sugar Tech, 16: 430-439.

- Sivaram, L. and Mukundan, U., 2003. In vitro culture studies on Stevia rebaudiana. In Vitro Cellular and Developmental Biology-Plant, 39: 520-523.

- Sreedhar, R.V., Venkatachalam, L., Thimmaraju, R., Bhagyalakshmi, N., Narayan, M.S. and Ravishankar, G.A., 2008. Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum, 52(2): 355-360.

- Tamura, Y., Nakamura, S., Fukui, H. and Tabata, M., 1984. Comparison of Stevia plants grown from seeds, cuttings and stem tip cultures for growth and sweet diterpene glycosides. Plant Cell Reports, 3: 180-182.

- Tanaka, O., 1982. Steviol-glycosides: New natural sweeteners. Trends in Analytical Chemistry, 1(11): 246-248.

- Wada, Y., Tamura, T., Kodama, T., Yamaki, T. and Uchida, Y., 1981. Callus culture and morphogenesis of Stevia rebaudiana (Bert.) Bertoni. Yukagawa, 36: 215-219.

- Yadav, A.K., Singh, S., Dhyani, D. and Ahuja, P.S., 2011. A review on the improvement of stevia (Stevia rebaudiana (Bertoni)). Canadian Journal of Plant Science, 91: 1-27.

Iranian Journal of Medicinal and Aromatic Plants Research

Effects of basal media and some physical parameters on micropropagation of stevia (Stevia rebaudiana Bertoni)

References

References

Volume 33, Issue 3 - Serial Number 83
August 2017
Pages 373-385

Effects of basal media and some physical parameters on micropropagation of stevia (Stevia rebaudiana Bertoni)

References

References

Volume 33, Issue 3 - Serial Number 83August 2017Pages 373-385

Volume 33, Issue 3 - Serial Number 83
August 2017
Pages 373-385