In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Department of Natural Resources, Semnan Agricultural and Natural Resources Research Center, Agricultural Research Education and Extension Organization (AREEO), Semnan, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

Abstract

In order to investigate the morphological reactions of Thymus kotschyanus Boiss. cutting to auxins (IAA and IBA) in different concentrations, an experiment was conducted in 2009 at the greenhouse conditions of Semnan Agriculture and Natural Resources Research Station. This research was conducted as a factorial experiment in a randomized complete block design with 3 replications and 20 observations per replication. The treatments included A: two types of auxin (Indole-acetic acid (IAA) and Indole-3-butyric acid (IBA)) and B: concentrations of auxin, (0, 100, 200, 300, 1000, 2000 and 3000 ppm). The traits studied were the root length and number, the percentage of rooting and dry root weight and new buds in plants. Results showed that the highest root dry weight and the root length were observed in the cuttings treated with 100 and 300 ppm of auxins. These concentrations (100 and 300 ppm) of auxins respectively stimulated root dry weight by about 3.6 and 3.25 times higher than control plants and increased the root length by about 150 and 90 percent more than control cuttings. The highest root length, root number, and number of new buds in the cuttings of Thymus kotschyanus were obtained due to the application of IAA as compared with IBA. Our findings confirm that the cuttings treated with 100 ppm of IAA can produce the highest quality transplants at commercial scale.

Keywords

- Avci, S., Cocu, S., Aasim, M., Sancak, C. and Ozcan, S., 2010. Effects of treating with auxin solutions on rooting of cuttings of sainfoin (Onobrychis viciifolia). Tropical Grasslands, 44: 123-127.
- Ahmadpour, A. and Nasabpour, M., 2011. Evaluation of cutting time and IBA concentrations on rooting of Euphorbia pulcherrima in Jiroft area. 7th Congress of Iranian Horticultural Science. Isfahan University of Technology, 21-24 October: 165-168.
- Arteca, R.N., 1996. Plant Growth Substances: Principles and Applications. New York: Chapman and Hall. Chicago Press, 345p.
- Balzich, F.A., 1989. Mineral nutrition and adventitious rooting: 61-69. In: Davis, T.D., Haissig, B.E. and Sankhla, N., (Eds.). Adventitious Root Formation in Cuttings. Dioscorides Press, Portland, 268p.
- Bartolini, G., Fabbri, A. and Tathini, M., 1986. Effects of phenolic acids and auxin on rooting of Olea europaea L. cuttings. Horticultural science, 21(3): 662-626.
- Bernath, J., 1993. Wild Growing and Cultivated Medicinal Plants. Mezzo Gazda press. Budapest, 150p.
- Coggeshall, R.C., 2002. Hybrid lilacs from softwood cuttings. American Nursery, 115: 5-7.
- Devries, D.P. and Dubois L.A.M., 1988. The effect of BAP and IBA on sprouting and adventitious root formation of “Amanda” Rose single node softwood cuttings. Scientiae Horticulture, 34: 115-121.
- Dewayne, L.I. and Yeager, T.H., 2003. Propagation of Landscape Plants. Institute of Food and Agricultural Sciences, University of Florida, CIR579, 15p.
- Farhadi, M., Heidari, H., Sharifani, M. and Kohrokhi, A., 2007. Influence of cutting time of stem and medium on rooting of maple (Acer velutinum Boiss.). Journal of the Iranian Natural Research, 60(2): 505-515.
- Ghahreman, A., 1994: Plant Systematics: Cormophytes of Iran (Vol. 3). Tehran University Press, Iran, 172p.
- Gaskell, M., 1988. Production of fresh culinary herbs in Central America for commercial export. The Herb, Spice, and Medicinal Plant Digest, 6(2): 1-10.
- Gautem, D.R. and Chauhan, J.S., 1992. Standardization of IBA concentrations and season on rooting of wild olive cuttings under intermittent mist. Horticultural Abstracts, 62: 111-113.
- Habibi Kotenaei, Sh., 2010. Effect of Auxin different concentrations on rooting of the semi-hardwood cutting in oleander plant. Journal on Plant Science Researches, 18(2): 36-46.
- Hartman, H.T., Kester, D.E. and Davies, F.T., 1975. Plant propagation principles and practices. Prentice Hall, Inc. Newjersey, 470p.
- Hornok, L., 1992. Cultivation and Processing of Medicinal Plants. Academy Press, Budapest, 402p.
- Iapichino, G., Arnone, C., Bertolino, M. and Amico Roxas, U., 2006. Propagation of three Thymus species by stem cuttings. Acta Horticulturae, 723: 411-414.
- Kan, Y., Kıvrak, N. and Kan, A., 2002. The effect of some growth regulators on the rooting of caper (Capparis ovate Desf. Var. canescens (cross) Heywood) cutting. Suleyman Demirel University, Agriculture Faculty Research, 16(30): 56-58.
- Kashefi, M., Zarei, H. and Bahadori, F., 2014a. The regulating effect of the growth of indole butyric acid and the time of stem cutting preparation on proliferation of damask rose ornamental shrub. Journal of Ornamental Plants, 4(4): 49-55.
- Kashefi, M., Zarei, H. and Bahadori, F., 2014b. The effect of indole butyric acid and the time of stem cutting preparation on propagation of damask rose ornamental shrub. Journal of Ornamental Plants, 4(4): 237-243.
- Khoshkhoi, M. and Panahi, R., 2009. Effect of auxin on rooting and flowering of Dianthus caryophyllus L. Journal of Horticultural Science and Technology, 1(3-4): 91-108.
- Khoshkhoi, M., Sheibani, B., Rouhani, A. and Tafazoli, E., 2004. A Principal of Horticulture. Center of Shiraz University, 490p.
- Khoshnevis, M., Kprori, S.A.A., Teimouri, M., Matinizadeh, M., Rahmani, A. and Shirvany, A., 2008. The effect of different treatments on rooting of Juniperus excelsa cutting. Iranian Journal of Forest and Poplar Research, 16(1): 158-167.
- Liu, J., Zhang, D., Zhang, B., Cao, J., Yang, Y. and Huang, H., 2010. Influence of different rooting hormone on cutting propagation of Michelia martini. Northern Horticulture, 5: 546-550.
- Liu, J. and Reid, D.M., 1992. Adventitious rooting in hypocotyls of sunflower (Heliantus annuuse) seedlings. VI. The role of change in endogenous free and conjugated indole-3-acetic acid. Physiology Plant Molecular Biology, 41: 455-496.
- Rey, C., 1995. Direct field sowing of thyme (Thymus vulgaris L.). Horticultural Abstracts, 65(8): 1375.
- Ruchala, S.L., Zhang, D., Mitchell, W. and Jianhau, L., 2002. Improving vegetative PropagationTechniques of Sweet Fern (Comptonia peregrina). Proceedings of the International Plant Propagators Society, 52: 381-387.
- Scalon, S., 2003. Auxins and boron on length of the biggest root and number of rooted cuttings of rosemary in two periods of planting. Revista Brasileira de Plantas Medicinais, 5(2): 71-76.
- Sefidkon, F., Jamzad, Z., Yavari, R., Nouri-Sharg, D. and Dabiri, M., 1999. Essential oil composition of Thymus kotschyanus Boiss. et Hohen. from Iran. Journal of Essential Oil Research, 11: 459-460.
- Zargari, A., 1997. Medicinal Plant (Vol. 4). Institute of Tehran University Press, 580p.