Antifungal effects of Zataria multiflora Boiss. and Thymus eriocalyx (Ronniger) Jalas essential oils on aflatoxin producing Aspergillus parasiticus.

Fakoor, M.H.; Allameh, A.; Rasooli, I.; Mazaheri, M.

Document Type : Research Paper

Authors

¹ M.Sc student of Islamic Azad University

² Tarbiat Modaress University

³ Shahed University

⁴ Institiute of standards and Industrial Research of Iran, Karaj

Abstract

The antifungal properties of Zataria multiflora Boiss. and Thymus eriocalyx (Ronniger) Jalas essential oils were studied on growth inhibition of Aspergillus parasiticus NRRL 2999. Minimal inhibitory (MIC) and fungicidal (MFC) and kinetics of fungal spore death as a result of exposure to the oils were studied. The oils were analyzed by GC and GC/MS and their chemical components were identified. 22 and 19 compounds were identified in Zataria multiflora Boiss. and Thymus eriocalyx (Ronniger) Jalas essential oils respectively. Eight compounds viz; α-thujene, α-pinene, sabinene, myrcene, p-cymene, 1,8-cineole, cis sabinene hydrate and thymol were common in both oils but in different concentrations. The results indicated powerful antifungal properties of both oils inhibiting growth and aflatoxin production that could be applied to food as preservatives.

Keywords

References

ـ زرگری، ع.، 1375. گیاهان دارویی. جلد اول، چاپ ششم، مؤسسه انتشارات و چاپ دانشگاه تهران، 948 صفحه.

ـ قهرمان، ا.، 1372. کروموفیتهای ایران (سیستماتیک گیاهی). جلد سوم، مرکز نشر دانشگاهی، تهران، 768 صفحه.

ـ صمصام شریعت، س.ه. و معطر، ف.، 1370. گیاهان و داروهای طبیعی. جلد دوم، انتشارات روزبهان، تهران، 288 صفحه.

- Adams, R.P., 1989. Identification of essential oils by Ion trap Mass Spectroscopy. Academic Press, San Diego, USA. 698 p.

- Baratta, T.M., Dorman, D.J.H., Deans, G.S., Fiqueiredo, C.A., Barroso, G.J. and Ruberto, G., 1998. Antimicribial and antioxidant properties of some commercial oils. Flavour and Fragrance Journal, 13: 235-244.

- Davies, N.W., 1998. Gas Chromatographic Retention Index of Monoterpenes and Sesquiterpenes on Methyl silicone and Carbowax 20 M phases. Journal of Chromatography, 503: 1-24.

- Eaton, D.J. and Groopman, J.D., 1994. The toxicology of aflatoxins; Human Health, Veterinary and Agricultural Significance. Academic Press, New York, 564 p.

- Juliano, C., Mattana, A. and Usai, M., 2000. Composition and in vitro antimicrobial activity of the essential oil of Thymus herba-barona loisel growing wold in Sardinia. Journal of Essential oil Research, 12: 516-522.

- Karaman, S., Digrak, M., Ravid, U. and Ilcim, A., 2001. Antibacterial and antifungal activity of the essential oils of Thymus revolutus celak from Turkey. Journal of Ethnopharmacology, 762: 183-186.

- Mahmoud, A.L., 1994. Antifungal action and antiaflatoxigenic properties of some essential oil constituents. Letters in Applied Microbiology, 2: 110-113.

-Nguefack, J., Leth, V., Amvam Zollo, P.H. and Mothur, S.B., 2004. Evaluation of five essential oils from aromatic plants of Cameroon for controlling food spoilage and mycotoxin producing fungi. International Journal of Food Microbiology, 94: 329-334.

- Ozcan, M., 1998. Inhibitoy effects of spice extracts on the growth of Aspergillus parasiticus NRRL 2999 strain. Z Naturforsch A, 207: 253-255.

- Pina-Vaz, C., Gone, A., Rodrigues, A., Pinto, E., Costa-de-oliveira, S., Tavares, C., Salgueiro, L., Cavaleiro, M.J. and Martinezde-Oliveira, J., 2004. Antifungal activity of Thymus oils and Their major compounds (JEADV). Journal of EuropeanAcademy of Dermatology and Venereology, 18: 73-78.

- Rasooli, I. and Mirmostafa, S.A., 2003. Bacterial susceptibility to and chemical composition of essential oils from Thymus kotschyanus and Thymus persicus. Journal of Agricultural and Food Chemistry.518: 2200-2205.

- Soliman, K.M. and Badeaa, R.I, 2002. Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food and Chemical Toxicology, 40: 1669-1675.

-Wistriech, G.A., 1997. Microbiology laboratory. Prentice Hall, 676 p.

Iranian Journal of Medicinal and Aromatic Plants Research

Antifungal effects of Zataria multiflora Boiss. and Thymus eriocalyx (Ronniger) Jalas essential oils on aflatoxin producing Aspergillus parasiticus.

References

References

Volume 23, Issue 2 - Serial Number 36
August 2007
Pages 269-277

Antifungal effects of Zataria multiflora Boiss. and Thymus eriocalyx (Ronniger) Jalas essential oils on aflatoxin producing Aspergillus parasiticus.

References

References

Volume 23, Issue 2 - Serial Number 36August 2007Pages 269-277

Volume 23, Issue 2 - Serial Number 36
August 2007
Pages 269-277