In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Islamic Azad University, Karaj Branch

2 Research Institute of Forests and Rangelands

Abstract

In order to study drought stress effect on quantitative and qualitative features of balm (Melissa officinalis L.) a research was conducted under field condition in Karaj, Iran in 2005. Experiment was conducted using Randomized Complete Block Design with 4 replications. Treatment included T1 (non stress), T2 (80%FC), T3 (60%FC), T4 (40%FC) and T5 (20%FC). Effect of drought stress on shoot yield, essential oil yield, percentage of essential oil, leaf and stem yield, height, tiller number, length and width of leaf, stem diameter and internodes was significant (α=0.001). Effect of drought stress on number of lateral stem was not significant. Comparison of treatment means showed that highest shoot yield was related to T1 (non Stress) (6469kg/ha), highest essential oil (12.970 kg/ha) and highest essential oil percentage (0.3012%) was related to T4 (40%FC) and T5 (20%FC), respectively. Highest height (65.32 cm), leaf length (6.27 cm) and width (4.58 cm) were related to T1 (non stress). Highest stem diameter was related to T5 (20%FC). It could be concluded that moderate drought stress is beneficial for balm essential oil.

Keywords

- ابرسجی، ق.ع.، 1384. تأثیر دور آبیاری بر روی عملکرد گیاه دارویی به‌لیمو. همایش ملی توسعه پایدار گیاهان دارویی، مشهد، 7-5 مرداد: 174-173.

- امیدبیگی، ر.، 1374. رهیافتهای تولید و فرآوری گیاهان دارویی. جلد اول، انتشارات فکر روز، 183 صفحه.

- پاک‌نژاد، ف.، 1384. بررسی اثر تنش خشکی بر شاخصهای فیزیولوژیکی عملکرد و اجزای عملکرد سه رقم گندم. پایان نامه دکتری زراعت، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات.
- خورگامی، ع.، 1376. بررسی برخی از پارامترهای فیزیولوژیکی و زراعی لوبیای چشم بلبلی در شرایط خشک. پایان نامه کارشناسی ارشد، دانشگاه آزاد اسلامی، واحد کرج.
- زرگری، ،ع. 1369. گیاهان دارویی. جلد سوم. انتشارات دانشگاه تهران. 923 صفحه.
- سرمدنیا، غ.، 1372. اهمیت تنش‌های محیطی در زراعت. مقالات کلیدی اولین کنگره زراعت و اصلاح نباتات ایران، دانشگاه تهران، 18-15 شهریور: 169-157.
- صفی‌خانی، ف.، 1385. بررسی جنبه‌های فیزیولوژیک مقاومت به خشکی در گیاه دارویی بادرشبو (Dracocephalum moldavica L.). پایان‌نامه دکتری، دانشگاه شهید چمران، مجتمع آموزش عالی کشاورزی و منابع طبیعی رامین.
- عزتی، پ.، 1381. بررسی تأثیر تراکم بر عملکرد و ماده مؤثره بادرنجبویه. پایان نامه کارشناسی ارشد زراعت، دانشگاه آزاد، واحد ورامین.
- کاظمی‌سعید، ف.، 1381. بررسی تأثیر تنش آبی و کود نیتروژنی بر میزان رشد عناصر معدنی محتوی بافت و اسانس در گیاه زیره سبز. پایان‌نامه کارشناسی ارشد علوم گیاهی، دانشگاه تربیت مدرس.
- Agata, I., Kusakabe, H., Hatano, T., and Nishibe, O.T., 1993. Melitric acids A and B, new trimeric caffeic acid derivatives from Melissa officinalis. Chemical and Pharmaceutical Bulletin, 41(9): 1608-1611.
- Bennett, C., 2003. Plant extract improves cognitive function in Alzheimer's disease Health News Co., UK, 172 p.
- Boon Jung, H. and Fukai, S., 1996. Effects of soil water deficit at different growth stages on rice growth and yield under upland condition. Field Crops Research, 48: 47-55.
- Carter, P., Sheaffer, C. and Voorhees, W., 1982. Root growth, herbage yield, and plant water status of alfalfa cultivars. Crop Sciences, 22: 425-427.
- Chatterjee, S., and Svoboda, K., 1995. Water stress effect on growth and yield of Cymbopogon sp. and its alleviation by n- triacontanol. Acta Horticulture, 39: 19-24.
- Kumari, B., Settu, A., and, Ramadhas, A., 1999. Studies on some morpho-physiological characters in fenugreek (Trigonnella foenum-gracum L.) to moisture stress. Advanced Science in Plants, 121: 171-176.
- Mikolajewicz, M., and Filoda, G., 1998. Septoria melissae Desm Control on common bahu (Melissa officinalis L.). Herba Poloinca, 44 (3): 172-174.
- Munn, S. and Alegre, L., 2000. The significance of beta carotene, alpha, tocopherol and the xanthophylls. cycle in droughted Melissa officinalis plant. Journal of plant physiology, 27(2): 139-146.
- Munne, S. and Alegre, L., 1999. Role of dew on the recovery of water stressed Melissa officinalis L. Journal of Plant Physiology, 154(5-6): 759-766.
- Munne, S., Schwarz, K., Alegre, L., Horvath, G., and Szigeti, Z., 1999. Alpha-tocopherol protection against drought, induced damage in Rosmarinus officinalis L and Melissa officinalis L. proceedings of an International workshop at Tata, Hungary, 23-26 August: 698-703.
- Repcak, M., Imrich, J. and Franekova, M., 2001. Umbelliferone, a stress metabolite of Chamomilla recutita L. Rauschert. Journal of Plant Physiology, 158: 1085-1087.
- Rizopoulou, S. and Diamantoglon, S., 1991. Water stress, induced diurnal variation in leaf water relation stomatal conductance, soluble sugar, lipids and essential oil content of Origanum majorana L. Journal of Horticultural Science, 66: 119 – 25.
- Saudan, S., Aparbsl, S., Singh, V., Man, S., Kailash, S., Singh, S., Singh, A., Singh, M., Singh, K., Kumar, S., Kukreja, A., Dwivedi, S. and Singh, AK., 2000. Studies on the frequency and time of irrigation application on herb and oil yield of palmarosa. Journal of Medicinal and Aromatic Plant Sciences, 22(1): 491-493.
- Schulz, H., Jobert, M. and Hubner, W., 1998. The quantitative EEG as a screening instrument to identify sedative effects of single doses of Plant extracts in comparison with diazepam. Phytomedicine, 5(6): 449-458.
- Sreevalli, Y., Baskaran, K., Chandrashekara, R., Kuikkarni, R., Sushil Hasan, S., Samresh, D., Kukre,j, Ashok, A., Sharma Singh, K., Srikant, S., and Rakesh, T., 2001. Preliminary observations on the effect of irrigation frequency and genotypes on yield and alkaloid concentration in Petriwinkle. Journal of Medicinal and Aromatic plant Sciences, 22: 356-358.
- Wake, G., Court, J., Pickering, A., Lewis, R., Wilkins, R., and Perry, E., 2000. CNS acetylcholine receptor activity in European medicinal Plantstraditionally used to improve failing memory. Journal of Ethno pharmacology, 69(2): 105-114.
- Weizman, Z., Alkrisnawi, S.and Golldfarb, D. and Bitran, C., 1993. Efficiency of herbal tea preparation in infantile colic. Journal of Pediatrics, 122(4): 650-652.
- Yanishlieva, N. and Marinova, E., 1998. Activity and mechanism of action of natural antioxidants in lipids. Recent Research Developments in oil Chemistry, 2(1): 1-14