In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 MSc. Student, School of Agricultural Engineering, Shahrood University of Technology, Shahrood, Iran

2 Agronomy and Plant Breeding Department, School of Agricultural Engineering, Shahrood University of Technology, Shahrood, Iran

3 School of Agricultural Engineering, Shahrood University of Technology, Shahrood, Iran

Abstract

In order to study the effect of different amounts of nitrogen fertilizer and foliar application of boron on some physiological traits in karela plants (Momordica charantia L.), a factorial experiment in a randomized complete block design with three replications was conducted at the Sharood University of Technology in 2013. The treatments consisted of three levels of nitrogen fertilizer: 75, 150 and 225 kg N.ha-1 (urea source) as the first factor and four foliar applications of boron including: 0, 1, 2 and 3 g per liter of boric acid as the second factor. The traits measured in this study included the length and diameter of fruit, fruit yield, chlorophyll "a","b", and carotenoids, the conent of sugar nitrate and nitrate reductase enzyme activity. The results showed that, nitrogen fertilizer except diameter of fruit had significant on all of attribute traits. Foliar application of boron in this study had significant effect on fruit yield, the content of chlorophyll "b" (P<0.05) in leaf, sugar and the amont of nitrate in leaf and fruit (P<0.01). Interaction between nitrogen fertilizer and foliar application of boron had significant effect on fruit yield and leaf nitrate content. The results showed that the highest fruit yield was obtained at 150 kg N.ha-1 and 2 g per liter of boron. In addition, the highest amount of nitrate in leaf and fruit tissues was obtained at 225 kg N. ha-1 treatment. Finally, 150 kg N. ha-1 fertilizer and 2 g per liter of boron was identified as the best treatment to obtain maximum yield and fruit quality.

Keywords

- خلدبرین، ب. و اسلام‌زاده، ط.، ۱۳۸۲. تغذیه معدنی گیاهان عالی. انتشارات دانشگاه شیراز، 229 صفحه.
- ملکوتی، م.ج. و تهرانی، م.م.، 1378. نقش ریزمغذی‌ها در افزایش عملکرد و بهبود کیفیت محصولات کشاورزی. انتشارات دانشگاه تربیت مدرس، تهران، 398 صفحه.
- Adiloglu, A. and Adiloglu, S., 2006. The effect of boron (B) application on the growth and nutrient contents of maize in zinc (Zn) deficient soil. Bulgarian Journal of Agricultural Science, 12: 387-392.
- Arnon, D.I., 1949. Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant physiology, 24: 1-15.
- Aroiee, H. and Omidbaigi, R., 2002. Effects of nitrogen fertilizer on productivity of medicinal pumpkin. Acta Horticulturae, 629: 415-419.
- Baranauskienne, R., Venskutonis, P.R., Viskelis, P. and Dambrausiene, E., 2003. Influence of nitrogen fertilizer on the yield and composition of thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry, 51(26): 7751-7758.
- Ben-Gal, A. and Shani, U., 2002. Yield, transpiration and growth of tomatoes under combined excess boron and salinity stress. Plant and Soil, 247(2): 211-221.
- Bergmann, W., 1992. Nutritional Disorders of Plants: Development, Visual and Analytical Diagnosis. Gustav Fischer Verlag Jena, Stuttgart, New York, 741p.
- Brown, P., Bellaloui, N., Wimmer, M.A., Bassil, E.S., Ruiz, J., Hu, H., Pfeffer, H., Dannel, F. and Römheld, V., 2002. Boron in plant biology. Plant Biology, 4(2): 205-223.
- Cabello, P., Roldán, M.D. and Moreno-Vivián, C., 2004. Nitrate reduction and the nitrogen cycle in archaea. Microbiology, 150: 3527-3546.
- Camacho-Cristóbal, J.J. and González-Fontes, A., 1999. Boron deficiency causes a drastic decrease in nitrate content and nitrate reductase activity, and increases the content of carbohydrates in leaves from tobacco plants. Planta, 209(4): 528-536.
- Camacho-Cristóbal, J.J. and González-Fontes, A., 2007. Boron deficiency decreases plasmalemma H+-ATPase expression and nitrate uptake, and promotes ammonium assimilation into asparagine in tobacco roots. Planta, 226: 443-445.
- Cataldo, D.A., Maroon, M., Schrader, L.E. and Youngs, V.L., 1975. Rapid colorimetric determination of nitrate in plant tissue by nitration of salicylic acid. Communications in Soil Science & Plant Analysis, 6: 71-80.
- Davis, J.M., Sanders, D.C., Nelson, P.V., Lengnick, L. and Sperry, W.J., 2003. Boron improves growth, yield, quality, and nutrient content of tomato. Journal of the American Society for Horticultural Science, 128: 441-446.
- Dubois, M., Gilles, J., Hamilton, J., Rebers, P. and Smith, F., 1956. Pfizer flocon 4800 procedure-phenol/sulfuric acid method. Analytical Chemistry, 28: 350-352.
- Eraslan, F., Inal, A., Gunes, A. and Alpaslan, M., 2007. Boron toxicity alters nitrate reductase activity, proline accumulation, membrane permeability, and mineral constituents of tomato and pepper plants. Journal of Plant Nutrition, 30: 981-994.
- Guvenc, I., 2002. Effect of nitrogen fertilization on growth, yield and nitrogen contents of radishes. Gartenbauwissenschaft, 67: 23-27.
- Hannaway, D. and Shuler, P., 1993. Nitrogen fertilization in alfalfa production. Journal of Production Agriculture, 6: 80-85.
- Heidari, M. and Mobasri-Moghadam, M., 2012. Effect of rate and time of nitrogen application on fruit yield and accumulation of nutrient elements in Momordica charantia. Journal of the Saudi Society of Agricultural Sciences, 11: 129-133.
- Kaiser, W., Weiner, H. and Huber, S., 1999. Nitrate reductase in higher plants: a case study for transduction of environmental stimuli into control of catalytic activity. Physiologia Plantarum, 105: 384-389.
- Lawal, A., 2000. Response of cucumber (Cucumis sativa L.) to intercropping with maize (Zea mays L.) and varying rate of FYM and inorganic fertilizer. Ph.D. dissertation, submitted to post graduate school, Ahmadu Bello University, Zaria, 267p.
- Liu, C.W., Sung, Y., Chen, B.C. and Lai, H.Y., 2014. Effects of Nitrogen Fertilizers on the Growth and NitrateContent of Lettuce (Lactuca sativa L.). International Journal of EnvironmentalResearch and Public Health, 11: 4427-4440.
- Lo´pez-Lefebre, L.R., Rivero, R.M., Garcı´a, P.C., Sa´nchez, E., Ruiz, J.M. and Romero, L., 2002. Boron effect on minral nutrient of tobacco. Journal of Plant Nutrition, 25: 509-522.
- Manuel-Ruiz, J. and Romero, L., 1999. Cucumber yield and nitrogen metabolism in response to nitrogen supply. Scientia Horticulturae, 82: 309-316.
- Marschner, H. and Rimmington, G., 1996. Mineral Nutrition of Higher Plants. Academic Press London, 889p.
- Matas, M., González-Fontes, A. and Camacho-Cristóbal, J., 2009. Effect of boron supply on nitrate concentration and its reduction in roots and leaves of tobacco plants. Biologia Plantarum, 53: 120-124.
- Nešić, Z., Tomić, Z., Krnjaja, V. and Tomašević, D., 2008. Nitrates in plants and soil after fertilization of grass-legume mixtures. Biotechnology in Animal Husbandry, 24: 95-104.
- Niyokuri, O., Rono, J., Fashaho, A. and Ogweno, J., 2013. Effect of different rates of nitrogen fertilizer on the growth and yield of Zucchini (Cucurbita pepo cv. Diamant L.) hybrid F1 in Rwandan high altitude zone. International Journal of Agriculture and Crop Sciences, 5: 54-62.
- Seth, K. and Aery, N.C., 2014. Effect of Boron on the Contents of Chlorophyll, Carotenoid, Phenol and Soluble Leaf Protein in Mung Bean, (Vigna radiata L.) Wilczek. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 84(3): 713-719.
- Sharifi-Rad, J., Sharifi-Rad, M. and Miri, A., 2013. Regulation of the Expression of Nitrate Reductase Genes in Leaves of Medical Plant, Foeniculum vulgare by Different Nitrate Sources. World Applied Sciences Journal, 28: 1311-1315.
- Shi, M. and Cheng, R., 2004. Effects of zinc and boron nutrition on balsam pear (Momordica charantia) yield and quality, and polyamines, hormone, and senescence of its leaves. Chinese Journal of Applied Ecology, 15(1): 77-80.
- Singh, B. and Usha, K., 2001. Effect of macro and micro-nutrient spray on fruit yield and quality of grape (Vitis vinifera L.) cv. Perlette. International Symposium on Foliar Nutrition of Perennial Fruit Plants, Meran, Italy, 11-15 September: 197-202.
- Stitt, M., Müller, C., Matt, P., Gibon, Y., Carillo, P., Morcuende, R., Scheible, W.R. and Krapp, A., 2002. Steps towards an integrated view of nitrogen metabolism. Journal of Experimental Botany, 53: 959-970.
- Sym, G.J., 1984. Optimisation of the in‐vivo assay conditions for nitrate reductase in barley (Hordeum vulgare L. cv. Igri). Journal of the Science of Food and Agriculture, 35: 725-730.
- Tori-Hudson, N., 2007. Bitter Melon: A Review of Its Indications, Efficacy, and Safety. American Journal of Health-System Pharmacy, 60: 356-359.
- Wang, Z. and Li, S., 2004. Effects of nitrogen and phosphorus fertilization on plant growth and nitrate accumulation in vegetables. Journal of Plant Nutrition, 27: 539-556.
- Yang, Z.O., Mei, X., Gao, F., Li, Y. and Guo, J., 2013. Effect of Different Nitrogen Fertilizer Types and Application Measures on Temporal and Spatial Variation of Soil Nitrate-Nitrogen at Cucumber Field. Journal of Environmental Protection, 4: 129-135.