In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

This experiment was done to investigate effect of Thyme (Thymus vulgaris L.) and Eucalyptus (Eucalyptus globulus Labill.)essential oils on gladiolus. Gladiolus is one of the most important ornamental plants whose production is threatened by Fusarium corm rot. Essential oils of Thyme and Eucalyptus are recommended as alternative for chemical fungicides for corms disinfection, but there is not considerable investigation about potential effects of these essential oils on growth and flowering of gladiolus in the greenhouse or field conditions. For this purpose, gladiolus corms were soaked at three concentrations of essential oils of Thyme (1000, 2000 and 3000 ppm) and Eucalyptus (2000, 4000 and 6000 ppm), and distilled water (control) for 14 hours before planting. The results showed that Thyme treatment at 1000 ppm increased the number of leaf and floret, root length, corm and cormlet production, and accelerated flowering. Other treatments delayed the sprouting and flowering time and significantly decreased sprouting percentage, height and number of leaves, root length, and chlorophyll content. Eucalyptus essential oil at concentrations more than 2000 ppm had negative effects ongladiolus, so that corm sprouting and flowering were inhibited at 6000 ppm and 4000 pp, respectively. Peroxidase enzyme activity increased after treatment with both essential oils but with a different trend. The activity of this enzyme in corms treated with thyme remained at a high level during the growth period; however, in corms treated with eucalyptus it was decreased significantly.

Keywords

- Arango, W.M., Ruiz J.M.A. and Jaramillo, C.A.P., 2011. Fungicidal activity of Eucalyptus tereticornis essential oil on the pathogenic fungus Fusarium oxysporum. Revista Cubana de Farmacia, 45: 264-274.
- Azirak, S. and Karaman, S., 2008. Allelopathic effect of some essential oils and components on germination of weed species. Acta Agriculturae, 58: 88-92.
- Babu, R.C. and Kandasamy, O.S., 1997. Allelopathic effect of Eucalyptus globulus Labill. on Cyperus rotundus L. and Cynodon dactylon L. Pers. Journal of Agronomy and Crop Science, 179(2): 123-126.
- Barrera-Necha, L.L., Garduno-Pizana, C. and Garcia-Barrera, L.J., 2009. In vitro antifungal activity of essential oils and their compounds on mycelial growth of fusarium oxysporum f. sp. gladioli (Massey) snyder and hansen. Plant Pathology Journal, 8: 17-21.
- Batish, D.R., Setia, N., Singh, H.P. and Kohli, R.K., 2004. Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide. Crop Protection, 23(12): 1209-1214.
- Bestwick, C.S., Brown, I.R. and Mansfield, J.W., 1998. Localized changes in peroxidase activity accompany hydrogen peroxide generation during the development of a non host hypersensitive reaction in Lettuce. Plant Physiology, 118: 1067-1078.
- Chandel, S. and De epika, R., 2010. Recent advances in management and control of Fusarium yellows in Gladiolus species. Journal of Fruit and Ornamental Plant Research, 18(2): 361-380.
- de Almeida, L.F., Frei, F., Mancini, E., De Martino, L. and De Feo, V., 2010. Phytotoxic activities of mediterranean essential oils. Molecules, 15(6): 4309-4323.
- Einhelling, F.A. and Rasmussen, J.A., 1979. Effects of three phenolic acids on chlorophyll content and growth of soybean and grain sorghum seedling. Journal of Chemical Ecology, 5:815-824.
- El-Zemity, S.R. and Ahmed, S.M., 2005. Antifungal activity of some essential oils and their major chemical constituents against some phytopathogenic fungi. Journal of Pest Control and Environmental Science, 13: 61-72.
- Guleria, S. and Kumar, A., 2006. Azadirachta indica leaf extract induces resistance in sesame against Alternaria leaf spot disease. Journal of Cell and Molecular Biology, 5(2): 81-86.
- Gupta, R.K. and Bansal, R.K., 2003. Comparative efficacy of plant leaf extracts and fungicides against Fusarium oxysporum Schlecht inducing fenugreek wilt under pot house condition. Annals of Applied Biology, 19: 35-37.
- Hanaa, R.M.F., Abdou, Z.A., Salama, D.A., Ibrahim, M.A.R. and Sror, H.A.M., 2011. Effect of neem and willow aqueous extracts on fusarium wilt disease in tomato Seedlings: Induction of antioxidant defensive enzymes. Annals of Agricultural Sciences, 56: 1-7.
- Kohli, R.K. and Singh, D., 1991. Allelopathic impact of volatile components from Eucalyptus on crop plants. Biologia Plantarum, 33: 475-483.
- Kumar, A., Shukla, R., Singh, P., Prasad, C.S. and Dubey, N.K., 2008. Assessment of Thymus vulgaris L. essential oil as a safe botanical preservative against postharvest fungi infestation of food commodities. Innovative Food Science Emerging Technologies Journal, 9(4): 575-580.
- Lichtenthaler, H.K., 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods in Enzymolog, 148: 350-382.
- Macias, F.A., Molinillo, J., Varela, R.M. and Galindo, J.C., 2007. Allelopathy a natural alternative for weed control. Pest Management Science, 63: 327-348.
- Malik, M.S., 2004. Effects of aqueous leaf exiracts of Eucalyptus globulus on germination and seedling growth of potato, maize and bean. Allelopathy Journal, 14: 213-220.
- Najafi Ashtiani, A., Assareh, M.H., Baghestani Meybodi, M.A. and Angaji, S.J., 2008. The Effects of methanolic extract of Eucalyptus camaldulensis Dehnh. on growth and germination rates of Chenopodium album L. Iranian Journal of Medicinal and Aromatic Plants, 24: 293-303.
- Nasir, I.A. and Riazuddin, S., 2008. New approaches to generate disease resistant gladiolus. World Journal of Microbiology and Biotechnology, 24: 367-378.
- Paudel, V.R. and Gupta, V.N.P., 2008. Effect of some essential oils on seed germination and seedling length of Parthenium hysterophorous L. Ecoprint: An International Journal of Ecology, 15: 69-73.
- Piraste, A., Emam, Y. and Saharkhiz, M.J., 2011. Use of allelopathic traits of several medicinal plants on some germination characteristics and early growth of wheat and wild Oat. Iranian Journal of Field Crops Research, 9: 95-104.
- Ram, R., Manuja, S., Dhyani, D. and Mukherjee, D., 2004. Evaluation of fortified fungicide solutions
on managing corm rot disease of gladiolus caused by Fusarium oxysporum. Crop Protection, 23(9): 783-788.
- Ramos-Garcia, M., Ortega-Centeno, S., Hernandez-Lauzardo, A.N., Alia-Tejacal, I., Bosquez-Molina, E. and Bautista-Banos, S., 2009. Response of gladiolus (Gladiolus spp) plants after exposure corms to chitosan and hot water treatments. Scientia Horticulturae, 121: 480-484.
- Riaz, T., Khan, S.N. and Javaid, A., 2008. Antifungal activity of plant extracts against Fusarium oxysporum-the cause of corm rot disease of gladiolus. Mycopathologia, 6: 13-15.
- Romagni, J.G., Allen, S.N. and Dayan, F.E., 2000. Allelopathic effects of volatile cineoles on two weedy plant species. Journal of Chemical Ecology, 26: 303-313.
- Saberi, M., Shahriari, A., Jafari, M., Tarnian, F. and Safari, H., 2012. Allelopathic effect of Thymus kotschyanus on seed germination and initial growth of Bromus inermis and Agropyron elongatum. Watershed Management Research (Pajouhesh & Sazandegi), 4(93): 18-25
- Sacchetti, G., Maietti, S., Mariavittoria, M., Scaglianti, M., Manfredini, S., Radice, M. and Bruni, R., 2005. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chemistry, 91: 621-632.
- Salmani, M., Afshari, H. and Mohammadi Moghadam M., 2014. Effects of plant essences on physiological characteristics of two cultivars of Iranian commercial pistachio nuts. Iranian Journal of Plant Physiology, 4: 1159-1166.
- Sellamuthu, P.S., Sivakumar, D., Soundy, P. and Korsten, L., 2013. Essential oil vapors suppress the development of anthracnose and enhance defense related and antioxidant enzyme activities in avocado fruit. Postharvest Biology and Technology, 81: 66-72.
- Singh, H.P., Batish, D.R., Setia, N. and Kohli, R.K. 2005. Herbicidal activity of volatile oils from Eucalyptus citriodora against Parthenium hysterophorus. Annals Applied Biology, 146: 89-94.
- Soliman, A.M.S. and Zatout, M.M., 2014. Comparative study on composition and allelopathic effect of volatile oils extracted from two Thymus species in Egypt. International Journal of the Enviorment and Water, 2(3): 59-66.
- Soltanipoor, M., Moradshahi, A., Rezaee, M., Kholdebarin, B. and Barazandeh, M., 2006. Allelopathic effects of essential oils of Zhumeria majdae on wheat (Triticumae stivum) and tomatto (Lycopersicon esculentum). Iranian Journal of Biology, 19: 19-28.
- Sonboli, A., Mirjalili M.H. and Yousefzadi M., 2006. Antimicrobial activity and composition of the essential oil of Cymbopogon olivieri (Boiss.) Bor from Iran. Iranian Journal of Pharmaceutical Research, 1: 65-68.
- Ziaebrahimi, L., Khavari-Nejad, R.A., Fahimi H. and Nejadsatari T., 2007. Effects of aqueous eucalyptus extracts on seed germination, seedling growth and activities of peroxidase and polyphenoloxidase in three wheat cultivar seedlings (Triticum aestivum L.). Pakistan Journal of Biological Sciences, 10(19): 3415-3419.