Optimization of induction and hairy root culture establishment of Teucrium chamaedrys L.

Bernousi, I.; Jafari, M.; Ahmadi Dizaji, J.

doi:10.22092/ijmapr.2016.106561

Document Type : Research Paper

Authors

Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Urmia, Urmia, Iran

https://doi.org/10.22092/ijmapr.2016.106561

Abstract

This research was aimed to investigate the effect of two infection methods (immersion and spray) and two Agrobacterium rhizogenes strains (strain A13 and GMI9534) to induce hairy roots on different explants (hypocotyl, cotyledon, leaf, and stem node) of Teucrium chamaedrys. Strain GMI9534 could not induce hairy roots in any of the explants, whereas strain A13 was only able to induce hairy roots in leaf and stem node explants. Infection by immersion method was more successful, with higher root induction efficiency (more than 73.3%), and leaf explants showed the highest induction frequency (83.3%). The transformed hairy root lines were confirmed by PCR using rolA and rolB gene-specific primers. Significant differences were shown among the 20 independent hairy root lines cultured on growth regulator-free MS solid medium for total root length and for the root branching. These variables were stable across subcultures and hence seven independent hairy root lines were selected based on these growth properties. Subsequently, the cultures for these hairy root lines were established in half-strength MS liquid medium to monitor their biomass production during a 90-day culture period. Considerable variations were observed in growth capacity among the lines. Line TC-HR-16 produced the highest fresh biomass (9100 mg/30 ml culture medium), a 455-fold increase over initial inoculum, during the 10-weeks culture period. The best-characterized hairy root lines, resulted in this study, can be used to improve the production of secondary metabolites of pharmaceutical values of T. chamaedrys.

Keywords

References

- Ahmadi Moghadam, Y., Piri, Kh., Bahramnejad, B. and Habibi, P., 2013. Methyl jasmonate and salicylic acid effects on the dopamine production in hairy cultures of Portulaca oleracea (Purslan). Bulletin of Environment, Pharmacology and Life Science, 2(6): 86-94.

- Alpizar, E., Dechamp, E., Lapeyre-Montes, F., Guilhaumon, C., Bertrand, B., Jourdan, C., Lashermes, P. and Etienne, H. 2008. Agrobacterium rhizogenes-transformed roots of coffee (Coffea arabica): conditions for long-term proliferation, and morphological and molecular characterization. Annals of Botany, 101(7): 929-940.

- Bagci, E., Yazgin, A., Hayta, S. and Cakilcioglu, U., 2010. Composition of the essential oil of Teucrium Chamaedrys L. (Lamiaceae) from Turkey. Journal of Medicinal Plants Research, 4(23): 2588-2589.

- Bensaddek, L., Villarreal, M.L. and Fliniaux, M.A., 2008. Induction and growth of hairy roots for the production of medicinal compounds. Electronic Journal of Integrative Biosciences, 3(1): 2-9.

- Camilleri, C. and Jouanin, L., 1991. The T_R-DNA region carrying the auxin synthesis genes of the Agrobacterium rhizogenes agropine-type plasmid pRiA4: nucleotide sequence analysis and introduction into tobacco plants. Molecular Plant-Microbe Interactions, 4: 155-162.

- Cho, H.J., Widholm, J.M., Tanaka, N., Nakanishi, Y. and Murooka, Y., 1998. Agrobacterium rhizogenes-mediated transformation and regeneration of the legume Astragalus Sinicus (Chinese milk). Plant Science, 138: 53-65.

- Doyle, J.J. and Doyle, J.L., 1990. Isolation of plant DNA from fresh tissue. Focus, 12: 13-15.

- Filippini, F., Lo Schiavo, F., Terzi, M., Costantino, P. and Trovato, M., 1994. The plant oncogene rolB alters binding of auxin to plant cell membranes. Plant Cell Physiology, 35: 767-771.

- Gupta, S.K., Liu, R.B., Liaw, S.Y., Chan, H.S. and Tsay, H.S., 2011. Enhanced tanshinone production in hairy roots of ‘Salvia miltiorrhiza Bunge’ under the influence of plant growth regulators in liquid culture. Botanical Study, 52(4): 435-443.

- Hasanloo, T., Rezazadeh, S. and Rahnama, H., 2008. Hairy root source for the production of valuable pharmaceutical compounds, Medicinal Plants, 29: 1-17.

- Hu, Z.B. and Du, M., 2006. Hairy root and its application in plant genetic engineering. Journal of Integrative Plant Biology, 48(2): 121-127.

- Kazemizadeh, Z. and Heidari Rikan, M., 2009. Investigation of chemical composition of essential oil of flowering branches of Teucrium chamaedrys L. subsp. Chamaedrys from Marmishoo valley, Urmia, http://www.azaranrc.com.

- Kim, Y.K., Li, X., Xu, H., Park, N.I., Uddin, M.R., Pyon, J.Y. and Park, S.U., 2009. Production of phenolic compounds in hairy root culture of tartary buckwheat (Fagopyrum tataricum Gaertn). Journal of Crop Science and Biotechnology, 12: 53-57.

- Lin, H.W., Kwok, K.H. and Doran, P.M., 2003. Development of Linum flavum hairy root cultures for production of coniferin. Biotechnology Letters, 25: 521-525.

- Lourenco, P.M.L., Castro, S.D., Martins, T.M., Clemente, A. and Domingos, A., 2002. Growth and proteolytic activity of hairy roots from Centaurea calcitrapa: effect of nitrogen and sucrose. Enzyme and Microbial Technology, 31: 242-249.

- Mathur, A., Ganwar, A., Mathur, A.K., Verma, P., Uniyal, G.C. and Lal, R.K., 2010. Growth kinetics and ginsenosides production in transformed hairy roots of American ginseng-Panax quinquefolium L. Biotechnolgy Letters, 32: 457-461.

- Md Setamam, N., Jaafar Sidik, N., Abdul Rahman, Z. and Che Mohd Zain, C.R., 2014. Induction of hairy roots by various strains of Agrobacterium rhizogenes in different types of Capsicum species explants. BMC Research Notes, 7: 414.

- Morteza-Semnani, K., Akbarzadeh, M. and Rostami, B., 2005. The essential oil composition of Teucrium Chamaedrys L. from Iran, Flavour and Fragrance Journal, 20: 544-546.

- Muselli, A., Desjobert, J.M., Paolini, J., Bernardini, A.F., Costa, J., Rosa, A. and Dessi, M.A., 2009. Chemical Composition of the essential oil of Teucrium Chamaedrys L. from Corsica and Sardinia. Journal of Essential Oil Research, 21(2): 138-143.

- Nader, B.L., Taketa, A.T., Pereda-Miranda, R. and Villarreal, M.L., 2006. Production of triterpenoids in liquid-cultivated hairy roots of Galphimia glauca. Planta Medica, 72: 842-844.

- Ooi, C.T., Syahida, A., Stanslas, J. and Maziah, M., 2013. Efficiency of different Agrobacterium rhizogenes strains on hairy root induction in Solanum mammosum. World Journal of Microbiology and Biotechnology, 29: 421-430.

- Park, S.U., Li, X., Eom, S.H., Lee, C.Y. and Lee, S.Y., 2010. E-P-Methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana miquel. Archives of Biological Sciences, 62:
649-652.

- Porter, J.R., 1991. Host range and implications of plant infections by Agrobacterium rhizogenes. Critical Reviews in Plant Sciences, 10: 387-421.

- Rechinger, K.H., 1982. Teucrium L.: 23-44. In: Rechinger, K.H., (Ed.). Flora Iranica. Akademische Druck und Verlagsanstalt, Wienna, 597p.

- Sadeghi, H., Jamalpoorb, S. and Shirzadib, M.H., 2014. Variability in essential oil of Teucrium polium L. of different latitudinal populations. Industrial Crops and Products, 54: 130-134.

- Samadi, A., Carapetian, J., Heidary, R., Jafari, M. and Hssanzadeh, A., 2012. Hairy root induction in Linum mucronatum ssp. an anti-tumor lignans production plant. Nothlae Botanicae Hortiagrobatanici Cluj-Napaca, 40(1): 125-131.

- Sivakumar, G., Yu, K.W. and Paek, K.Y., 2005. Production of biomass and ginsenoides from adventitious roots of Panax ginseng in bioreactor cultures. Engineering in Life Sciences, 5(4): 333-342.

- Soleimani, T., Keyhanfar, M., Piri, K. and Hasanloo, T., 2012. Morphological evaluation of hairy roots induced in Artemisia annua L. and investigating elicitation effects on the hairy roots biomass production. International Journal of Agriculture: Research and Review, 2: 1005-1013.

- Stankovic, M.S., Curcic, M.G., Zizic, J.B., Topuzovic, M.D., Solujic, S.R. and Markovic, S.D., 2011. Teucrium plant species as natural sources of novel anticancer compounds: antiproliferative, proapoptotic and antioxidant properties. International Journal of Molecular Science, 12: 4190-4205.

- Suza, W., Harris, R.S. and Lorence, A., 2008. Hairy Roots: From high-value metabolite production to phytoremediation. Electronic Journal of Integrative Biosciences, 3(1): 57-65.

- Torkamani, M.R., Jafari, M., Abaspour, N., Heidary R. and Safaie, N., 2014. Enhanced production of velerenic acid in hairy root culture of Valeriana officinalis by elicitation. Central European Journal of Biology, 9: 853-863.

- Udomsuka, L., Jarukamjorna, K., Tanakab, H. and Putaluna, W., 2009. Isoflavonoid production in a hairy roots culture of Pueraria candollei. Zeitschrift für Naturforschung, 64: 687-691.

- Veena, V. and Taylor, C.G., 2007. Agrobacterium rhizogenes: recent developments and promising applications. In Vitro Cell Developmental Biology-Plant, 43: 383-403.

- Wang, B., Zhang, G., Zhu, L., Chen, L. and Zhang, Y. 2006. Genetic transformation of Echinacea purpurea with Agrobacterium rhizogenes and bioactive ingredient analysis in transformed cultures. Colloids and Surfaces B: Biointerfaces, 53: 101-104.

- Welander, M. and Zhu, L.H., 2006. Rol genes: molecular biology, physiology, morphology, breeding uses: 79-97. In: Janick, J., (Eds.). Plant Breeding Reviews, Wiley, NewYork, 394p.

- Yoshimatsu, K., Sudo, H., Kamada, H., Kiuchi, F., Kikuchi, Y., Sawada, J. and Shimomura, K. 2004. Tropane alkaloid production and shoot regeneration in hairy and adventitious root cultures of Duboisia myoporoides-D. leichhardtii hybrid. Biological and Pharmaceutical Bulletin, 27: 1261-1265.

Iranian Journal of Medicinal and Aromatic Plants Research

Optimization of induction and hairy root culture establishment of Teucrium chamaedrys L.

References

References

Volume 32, Issue 2 - Serial Number 76
June 2016
Pages 264-280

Optimization of induction and hairy root culture establishment of Teucrium chamaedrys L.

References

References

Volume 32, Issue 2 - Serial Number 76June 2016Pages 264-280

Volume 32, Issue 2 - Serial Number 76
June 2016
Pages 264-280