Evaluation of withaferin A content in different accessions and in in vitro cultures of Withania coagulans (Stocks) Dunal

Bagheri, A.; Valizadeh, M.; Sharifi, A.; Senthil, K.

doi:10.22092/ijmapr.2016.106558

Document Type : Research Paper

Authors

¹ Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

² Department of Medicinal Plants, College of Agriculture, High Educational Complex of Saravan, Iran

³ Department of Plant Tissue Culture and Micropropagation, Iranian Academic Center for Education, Culture and Research, Branch of Mashhad, Mashhad, Iran

⁴ Department of Biochemistry, Biotechnology and Bioinformatics, Avinashilingam Institute of Home Science and Higher Education for Women, Tamil Nadu, India

https://doi.org/10.22092/ijmapr.2016.106558

Abstract

In recent years, W. coagulans (Stocks) Dunal (Fam. Solanaceae) has gained much attention, owing to the presence of a large number of steroidal lactones known as withanolides. Out of the several withanolides isolated from W. coagulans, the biological compound, withaferine A is pharmacologically important that due to the signiﬁcant and speciﬁc therapeutic action in cancer, Parkinson and Alzheimer’s disease. The present study was, therefore, undertaken to make an assessment of withaferin A content in the roots of different accessions of W. coagulans and in in vitro cultures. The seeds of five wild accssesion (USB001-5) were sown in greenhous. The cell suspension cultures were initialized from leaf explants derived callus on Murashige and Skoog (MS) medium, supplemented with 30 gL^-1 sucrose (w/v), 2.0mg/l 2,4-D and 0.5 mg/l kinetin (Kin). Adventitious roots were induced directly from leaf segments on half strength MS medium (0.8% agar) with 2 mgl^-1 indole-3-butyricacid (IBA) and 30 gl^-1 sucrose. The withaferin A content was evaluated by TLC and HPLC method using standard withaferin-A compound. The results showed the presence of withaferin A in all accessions (21.01-44.54 µg/g D.W). In addition, there was significant differences among accession (P<0.05). USB005 was found to have the highest withaferin A content (44.54µg/g D.W) and was selected for in vitro study. The results of in vitro study showed that withaferin A accumulation was higher in adventitious roots (21.40±1.67 in 4 weeks and 66.73±0.86 in 8 weeks old cultures) compared to cell suspension culture (6.62±2.01). Nearly, adventitious root having withaferin A content 10 and 1.5-fold higher when compared with the cell suspension and in vivo roots, respectively. Thus, our study demonstrates the in vitro root cultures potential for large-scale production of withaferin A.

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Evaluation of withaferin A content in different accessions and in in vitro cultures of Withania coagulans (Stocks) Dunal

References

References

Volume 32, Issue 2 - Serial Number 76
June 2016
Pages 231-244

Evaluation of withaferin A content in different accessions and in in vitro cultures of Withania coagulans (Stocks) Dunal

References

References

Volume 32, Issue 2 - Serial Number 76June 2016Pages 231-244

Volume 32, Issue 2 - Serial Number 76
June 2016
Pages 231-244