Document Type : Research Paper

Authors

• M.H. Abdolmohammadi ¹
• A. Dadkhah ²
• M. Babaei ³

¹ Department of Clinical Biochemistry, School of Medicine, Qom University of Medical Sciences, Qom, Iran

² Department of Medicine, Faculty of Medicine, Islamic Azad University, Qom Branch, Qom, Iran

³ Department of Chemistry, Faculty of Science, Islamic Azad University, Qom Branch, Qom, Iran

Abstract

Carbon tetra chloride converts to CCL₃O by cytochrom P 450 that conjugate with glutathione by GST, leading to GSH depletion and oxidative injuries. Therefore, the compounds with antioxidant activity can prevent oxidative damages induced by CCL₄. In this study, the rats were divided into five groups treated with CCL₄, (2ml/kg bw), essential oil (100 and 200 mg/kg bw) and BHT (10mg/kg bw) (ip). In following, the liver and blood were removed after administrations. The activity of GST, reduced glutathione (GSH), and lipid peroxidation (LP) levels were measured in liver homogenate. In addition, total antioxidant activity and the level of hepatic marker (AST, ALT, ALP and bilirubin) were measured in plasma of rats. The results showed that CCL₄ caused GSH depletion and reduction of GST activity in liver, leading to increased liver LP level and transaminase activities (AST and ALT) in plasma. Treatments of rats with essential oil as same as BHT prevent the changes of these parameters. We can conclude that CCL₄ induces hepatic oxidative damages in rats. Treatment of rats with essential oils, probably due to its antioxidant activity, can partly prevent the induction of liver injuries.

Keywords

• Zataria multiflora Boiss
• carbon tetrachloride
• liver
• rat