In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Research Institute of Forests and Rangelands, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

2 Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

This research was conductedon Rosa damascena Mill. in a randomized complete block design with three replications in the Alborz research station during 2012-2013. Treatments included nano chelated iron fertilizer at six levels: (0 (control), two times foliar spray (8g/plant), three times foliar spray (12g/plant), one time soil application (8g/plant), one time soil application (12 g/plant). The first foliar spray was done at blossoming stage. The second and third applications were 10 and 20 days after blossoming. Soil application was at blossoming stage accompanied by irrigation. The essential oil of samples was extracted by Clevenger and water distillation method for three hours. GC and GC/MS were used to determine the percentage and essential oil compounds of fresh flowers. According to the results of variance analysis, significant differences were found for the essential oil percentage, citronellol, icosane, octadecanol, Henicosane, Tricosane, Tetrosane, geraniol, and Tridane (p<0.01). Mean comparison of treatments indicated that the essential oil percentage of fresh flowers by three-times foliar spray (0.071%) was more than that of one time foliar spray (0.061%) and one time soil application (12 g/plant) (0.051%) and other treatments. The highest Citronellol was obtained in one-time foliar spray (1.17%) and the lowest in control treatment (0.36%). In addition, the highest tricosane (33.77%) was obtained from the use of 12g chelated iron in soil. The results of correlation analysis showed that there was a significant positive correlation between essential oil percentage of fresh flower and essential oil stability at refrigerator as well as dried flower at shadow. A significant negative correlation was also found between citronellol and ecosane. Results indicated that fertilizers could affect the percentage and compounds of essential oil in Rosa damascene.

Keywords

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