In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 Department of Physical Education, kharazmi University, Tehran, Iran

2 Department of Physical Education and Sport Science, Borujerd branch, Islamic Azad University, Borujerd, Iran

3 Department of Physical Education, Shahid Rajaee Teacher Training University, Tehran, Iran

Abstract

The purpose of this study was to investigate the effects of downhill running and short term Melissa officinalis L. (MO) supplementation on CRP(circulating reactive protein) and the amount of perceived soreness (pain) in the male athletes. For this purpose, 20 male athletes were randomly divided into two groups of 10 (supplement and placebo). Serum CRP concentration and level of perceived thigh pain before and after the supplementation were measured. After collecting the data, ANOVA for repeated evaluation of time series and the Bonferroni post hoc test were significant (p<0.05), and t-test was used to evaluate difference between-groups. The findings of the study were that total serum CRP was increased less in the supplement group than that of placebo (p=0.03). The amount of perceived soreness was lower in the supplement group (p=0.03). It is concluded that the herbal supplement can decrease perceived thigh pain rate and elevated serum CRP levels after downhill running.

Keywords

- Akhondzadeh, S., Noroozian, M., Mohammadi, M., Ohadinia, S., Jamshidi, A. and Khani, M., 2003. Melissa officinalis extract in the treatment of patients with mild to moderate Alzheimer's disease: a double blind, randomised, placebo controlled trial. Journal of Neurology Neurosurgery Psychiatry, 74(7): 863-866.
- Alessio, H.M., 1993. Exercise-induced oxidative stress. Journal of Medicine and Science in Sports and Exercise, 25(2): 218-224.
- Bloomer, R.J., Goldfarb, A.H. and McKenzie, M.J., 2006. Oxidative stress response to aerobic exercise: comparison of antioxidant supplements. Journal of Medicine Science Sports Exercise, 38(6):1098-1105.
- Bryer, S.C. and Goldfarb, A.H., 2006. Effect of high dose vitamin C supplementation on muscle soreness, damage, function, and oxidative stress to eccentric exercise. International Journal of Sport Nutrition and Exercise Metabolism, 16(3): 270-280.
- Cannavino, C.R., Abrams, J., Palinkas, L.A., Saglimbeni, A. and Bracker, M.D., 2003. Efficacy of transdermal ketoprofen for delayed onset muscle soreness. Clinical Journal of Sport Medicine, 13(4): 200-208.
- Clarkson, P.M. and Hubal, M.J., 2002. Exercise-induces muscle damage in humans. American journal of Physical medicine Rehabilitation, 81(11): 552-569.
- Cleak, M.J. and Eston, R.G., 1992. Delayed onset muscle soreness: mechanisms and management. Journal of Sports Sciences, 10(4): 325-341.
- Dehghan, G.H., Ebrahimi, S., Shaghaghi, M., Jafari, A., Mohammadi, M., Badalzadeh, R. and Fallah, S., 2001. Antioxidant effect of Cinnamomum zeylanicum supplementation following an exhaustive exercise bout in male rats. Practical Research Center of Tabriz Medical University, 13(5): 21-28.
- Dimitrov, J.D., Vassilev, T.L., Andre, S., Kaveri, S.V. and Lacroix-Desmazes, S., 2008. Functional variability of antibodies upon oxidative processes. Autoimmunity Reviews, 7(7): 574-578.
- Fazli, D., Malekirad, A.A., Pilehvarian, A.A. and Salehi, H., 2012. Effects of Melissa officinalis L. on oxidative status and biochemical parameters in occupationally exposed workers to aluminum: a before after clinical trial. International Journal of Pharmacology, 8(5):455-458.
- Goldfarb, A.H., 1999. Nutritional antioxidants as therapeutic and preventive modalities in exercise induced muscle damage. Canadian Journal of Applied Physiology, 24(3): 249-266.
- Holt, E.M., Steffen, L.M., Moran, A., Basu, S., Steinberger, J., Ross, J.A., Hong, C.P. and Sinaiko, A.R., 2009. Fruit and vegetable consumption and its relation to markers of inflammation and oxidative stress in adolescents. Journal of the American Diabetic Association, 109(3): 414-421.
- Jackson, A.S. and Pollock, M.L., 1978. Generalized equations for predicting body density for men. British Journal of Nutrition, 91: 161-168.
- Jackson, A.S. and Pollock, M.L., 1985. Practical assessment of body composition. The Physician and Sportsmedicine, 13: 76-90.
- Jafari, A., Etemadian, F. and Bashiri, J., 2012. The effect of creatine monohydrate supplementation on inflammatory response after downhill running in male fitted mountain climbers. Journal of Kerman University of Medical Sciences, 19(4): 354-366.
- Karvonen, J. and Vuorimaa, T., 1988. Heart rate and exercise intensity during sport activities. Journal of Sport Medicine, 5(5): 303-311.
- Liang, M.T., Allen, T.W., McKeigue, M.E., Kotis, A. and Gierke, L.W., 2001. Effect of cooling on muscular health prior to running a marathon. Journal of American Osteopathology Association, 101(4): 219-225.
- Malekirad, A.A., Ranjbar, A., Rahzani, K., Kadkhodaee, M., Rezaie, A., Taghavi, B. and Abdollahi, M., 2005. Oxidative stress in operating room personnel: occupational exposure to anesthetic gases. Human and Experimental Toxicology, 24(11): 597-601.
- Masayoshi, Y. and Toshio, Y.A., 2000. Nationwide survey of middle-aged Japanese mountaineers;
from a viewpoint of preventing accidents. Japanese Journal of Mountain Medicine, 20(1): 65-73.
- Maughan, R.J., Donnelly, A.E., Gleeson, M., Whiting, P.H., Walker, K.A. and Clough, P.J., 1989. Delayed-onset muscle damage and lipid peroxidation in man after a downhill run. Muscle & Nerve Journal, 12(4): 332-336.
- Meyer, T., Gabriel, H.H., Ratz, M.A., Muller, H.J. and Kindermann, W.I., 2001. Anaerobic exercise induces moderate acute phase response. Medicine and science in sports and exercise, 33(4): 549-555.
- Mier-Cabrera, J., Aburto-Soto, T., Burrola-Méndez, S., Jiménez-Zamudio, L., Tolentino, M.C., Casanueva, E. and Hernández-Guerrero, C., 2009. Women with endometriosis improved their peripheral antioxidant markers after the application of a high antioxidant diet. Biology and Endocrinology Journal, 54(7): 1-11.
- Milias, G.A., Nomikos, T., Fragopoulou, E., Athanasopoulos, S. and Antonopoulou, S., 2005. Effects of eccentric exercise-induced muscle injury on blood levels of platelet activating factor (PAF) and other inflammatory markers. European Journal of Applied Physiology, 95(5): 504-513.
- Ranchordas, M.K., Rogerson, D. and Soltani, H., 2012. Antioxidants for preventing and reducing muscle soreness after exercise (Protocol). Published by JohnWiley & Sons, Ltd.
- Rostami, S., Moemeni, Z., Rasuli, M. and Ghayur, N., 2010. Comparison of antioxidant effect of Cvitamin and Melissa officialis on Lead-induced learning disorders in rats. Journal of Shahed University, 17(6): 1-9.
- Scharhag, J., Meyer, G.T., Gabriel, H.H.W., Schlick, B., Faude, O. and Kindermann, W., 2005. Does prolonged cycling of moderate intensity affect immune cell function?. British Journal of Sport Medicine, 39: 171-177.
- Shafat, A., Butler, P., Jensen, R.L. and Donnelly, A.E., 2004. Effects of dietary supplementation with vitamins C and E on muscle function during and after eccentric contractions in humans. European Journal of Applied Physiology, 93(1–2): 196-202.
- Simpson, R.J., Wilson, M.R., Black, J.R., Ross, J.A., Whyte, G.P., Guy, K. and Florida-James, G.D., 2005. Immune alterations, lipid peroxidation, and muscle damage following a hill race. Canadian Journal of Applied Physiology, 30: 196-211.
- Smith, L.L., 1991. Acute inflammation: the underlying mechanism in delayed onset muscle soreness?. Medicine and Science in Sports and Exercise, 23(5): 542-551.
- White, J.P., Wilson, J.M., Austin, K.G., Greer, B.K., John, N.S. and Panton, L.B., 2008. Effect of carbohydrate-protein supplement timing on acute exercise-induced muscle damage, Journal of the International Society of Sports Nutrition, 31: 315-326.
- Yengi Maleki, G. and Hematfar, A., 2014. The effects of short-term extract of Melissa officinalis supplement on hip circumference (swelling) after aerobic exercise in a negative slope. Sports Medicine Journal, 10(2): 23-35.
- Zeraatpishe, A., Oryan, S., Bagheri, M.H., Pilevarian, A.A., Malekirad, A.A., Baeeri, M. and Abdollahi, M., 2011. The effect of Melissa officinalis L. (lemon balm) infusion on enzymatic antioxidants activity in radiology staff. Toxicology and Industrial Health, 27(3): 205-212.