In collaboration with Scientific Association of Iranian Medicinal Plants

Document Type : Research Paper

Authors

1 MSc. Student, Payam Noor University, Tehran, Iran

2 Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran

3 Payam Noor University, Tehran, Iran

Abstract

The Achillea genus has 19 herbaceous aromatic species in Iran, of which six species are endemic. One of the native species of this genus is Achillea nobilis. In traditional medicine, different parts of Achillea species are used because of antiseptic, anti-inflammatory, antihistamine and antioxidant properties. In this research, the seeds of Achillea nobilis L. were collected from Hamedan province and cultivated in the field of Alborz Research Station, Karaj, Iran.  In order to comparing the essential oil content and composition, flowering shoots and individual plant parts (flower, leaf and stem) were collected in full flowering stage. The plant materials were dried at shade and their essential oils were obtained by hydro-distillation. The oils were analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The highest oil yield was obtained from flowers (1.32%) and the lowest from stems (0.14%). The oil yield of flowering shoot and leaf was 0.85% and 0.62%, respectively. The main components of flower and leaf oil were cis-chrysanthenol (47.1% and 50.8%) and α-thujone (8.8% and 9.1%), respectively. The essential oil of stem with 19.7% cubenol, 19.2% indipone, 15.4% cis-chrysanthenol and 9.9% intermedol was completely different with leaf and flower oils. The major compounds of flowering shoot's essential oil were cis-chrysanthenol (41.7%), α-thujone (10.2%) and camphor (8.1%). According to these results, the studied sample could be introduced as a new chemotype of Achillea nobilis with higher percentage of cis-chrysanthenol. In addition, since the essential oil composition of flowering shoot is near to leaf and flower oils, the distillation of flowering shoot of this chemotype is recommended for obtaining more essential oil content.

Keywords

- آزادبخت، م.، 1378. رده‌بندی گیاهان دارویی. انتشارات تیمورزاده، تهران، 270 صفحه.
- امیدبیگی، ر.، 1384. تولید و فرآوری گیاهان دارویی (جلد دوم). انتشارات آستان قدس رضوی، به نشر، مشهد، 438 صفحه.
- پارسا، ا.، 1375. فلور ایران (جلد سوم). انتشارات مؤسسه تحقیقات جنگلها و مراتع کشور، تهران، 227 صفحه.
- زرگری، ع.، 1371. گیاهان دارویی (جلد سوم). انتشارات دانشگاه تهران، تهران، 925 صفحه.
- شریعتی، م.، طهماسب، آ. و مدرس هاشمی، س.م.، 1381. بررسی تأثیر تیمارهای مختلف بر شکستن خواب بذر گیاه بومادران (Achillea millefolium). پژوهش و سازندگی، 15(4-3): 8-2.
- غنی، ع.، عزیزی، م.، حسنزاده خیاط، م. و پهلوان پورفرد جهرمی، ع.ا، 1387. مقایسه درصد و اجزای اسانس دو توده وحشی بومادران (Achillea wilhelmsii Koch.). علوم آب و خاک (علوم و فنون کشاورزی و منابع طبیعی)، 45: 589-581.
- کاظمی‌زاده، ز.، مرادی، ا. و یوسفی، م.، 1390. بررسی ترکیب‌های شیمیایی اسانس گل و برگ بومادران تماشایی (Achillea nobilis L. subsp. neilreichii) رویش یافته در استان گیلان. گیاهان دارویی، 38: 162-156.
- مظفریان، و.، 1381. فرهنگ نامهای گیاهان ایران. انتشارات فرهنگ معاصر، تهران، 671 صفحه.
- مظفریان، و.، 1387. فلور ایران: تیره کاسنی. انتشارات مؤسسه تحقیقات جنگلها و مراتع کشور، تهران، 443 صفحه.
- Azizi, M., Chizzola, R., Ghani, A. and Oroojalian, F., 2010. Composition at different development stages of the essential oil of four Achillea species grown in Iran. Natural Product Communications, 5: 283-290.
- Carnat, A.P., Madesclaire, M., Chavignon, O. and Lamaison, J.L., 1992. Cis-Chrysanthenol, a main component in essential oil of Artemisia absinthium L. growing in Auvergne (Massif Central), France. Journal of Essential Oil Research, 4(5): 487-490.
- Chen, J., Cantrell, C.L., Duke, S.O. and Allen, S.O., 2008. Repellency of callicarpenal and intermedeol against workers of imported fire ants (Hymenoptera: Formicidae). Journal of Economic Entomology, 101(2): 265-271.
- Demirci, F., Demirci, B., Gorboz, L., Yesilada, E. and Baser K.H.C., 2009. Characterizathon and biological activity of Achillea teritifolia willd and A. nobilis L. subsp neilreichi (Kerner) formanek essential oils. Turkish Journal of Biology, 33: 129-136.
- Jeong, S.H., Koo, S.J., Choi, J.H., Park, J.H., Ha, J., Park, H.J. and Lee, K.T., 2002. Intermedeol isolated from the leaves of Ligularia fischeri var. spiciformis induces the differentiation of human acute promyeocytic leukemia HL-60 Cells. Planta Medica, 68(10): 881-885.
- Karabay-Yavasoglu, N.U., Karamenderes, C., Baykan, S. and Apaydin, S., 2007. Antinociceptive and anti-inflammatory activities and acute toxicity of Achillea nobilis subsp neilreichii extract in mice and rats. Pharmaceutical Biology, 45: 162-168.
- Karamenderes, C. and Apaydin, S., 2003. Antispasmodic effect of Achillea nobilis L. subsp. sipylea (O. Schwarz) Bassler on the rat isolated duodenum. Journal of Ethnopharmacology, 84: 178-179.
- Kim, K.J., Kim, Y.H., Yu, H.H., Jeong, S.I., Cha, J.D., Kil, B.S. and You, Y.O., 2003, Antibacterial activity and chemical composition of essential oil of Chrysanthemum boreale. Planta Medica, 69(3):
274-277.
- Lutgen, P., 2013. Artemisia ketone, phytosterols and lipid metabolism. Food and Chemical Toxicology, 58: 37-49.
- Nai-Yun, J., Wei, W., Xiu-Li, Y. and Qin-Zhao, X., 2009. A New Epoxy-cadinane Sesquiterpene from the Marine Brown Alga Dictyopteris divaricata. Drugs, 7(4): 600-604.
- Rustaiyan, A., Masoudi, S., Ezatpour, L., Taherkhani, M. and Aghajani, Z., 2011. Composition of the essential oils of Anthemis hyalina DC., Achillea nobilis L. and Cichorium intybus L. three Asteraceae herbs growing wild in Iran. Journal of Essential Oil Bearing Plants, 14(4): 472-480.
- Santos F.A. and Rao V.S.N., 2000. Antiinflammatory and antinociceptive effects of 1,8-cineole a terpenoid oxid present in many plant essential oils. Phytotherapy Research, 14(4): 240-244.
- Takao, Y., Kuriyama, I., Yamada, T., Mizoguchi, H., Yoshida, H. and Mizushina, Y., 2012. Antifungal properties of Japanese cedar essential oil from waste wood chips made from used sake barrels. Molecular Medicine Reports, 5(5): 1163-1168.
- Yashphe, J., Feuerstein, I., Barel, S. and Segal, R., 1987. The antibacterial and antispasmodic activity of Artemisia herba-alba Asso. II. examination of essential oils from various chemotypes. Pharmaceutical Biology, 25(2): 89-96.