Antispasmodic activity of Physalis alkekengi L. leaf hydroalcoholic extract on rat ileum

Gharib Naseri, M.K.; Handali, S.; Hoseini, H.

Document Type : Research Paper

Authors

Department of Physiology, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran

Abstract

Physalis alkekengi L. fruit (from Solanaceae) shows diuretic and laxative activities. The aim of present study was to investigate the antispasmodic effect of hydroalcoholic leaf extract on rat ileum contractility. Leaf powder of Physalis alkekengi was extracted by maceration in 70% alcohol for 72 h. A terminal portion of male Wistar rat ileum was dissected and its contractions were recorded isotonically in an organ bath containing Tyrode solution (37 ºC, pH 7.4) under 1 g tension. The results showed cumulative concentrations of the extract (0.25-2 mg/ml) reduced the ileum contraction induced by KCl (60 mM) or by carbachol (10μM) dose-dependently (P<0.0001). The antispasmodic effect of extract at 1 mg/ml was attenuated (P<0.01) by incubation with propranolol (1μM, 30 min) but was not affected by tissue incubation with naloxone (1μM, 30 min) and L-NAME (100μM, 20 min). In Ca²⁺-free with high K⁺ (60mM) Tyrode solution, the extract (0.5-2mg/ml) reduced the ileum contractions induced by cumulative concentrations of CaCl₂ dose-dependently (PPhysalis alkekengi leaf hydroalcoholic extract inhibited rat ileum contractions neither by involving opioid receptors nor by NO synthesis. It seems that partially β-adrenoceptors and mainly the calcium channels are involved in the spasmolytic effect of the extract.

Keywords

References

- زرگری، ع.، 1371. گیاهان دارویی. جلد سوم، انتشارات دانشگاه تهران، تهران، 598- 595.

- Austin, D.F., 1991. Ipomoea littoralis (Convolvulaceae)-taxonomy, distribution and ethnobotany. Economic Botany, 45: 251-256.

- Black, R.E., Brown, K.H., Becker, S. and Yunus, M., 1982. Longitudinal studies of infectious diseases and physical growth of children in rural area of Bangladesh. I. Patterns of morbidity. American Journal of Epidemiology, 115: 305-314.

- Bolton, T.B., 1979. Mechanisms of action of transmitters and other substances on smooth muscle. Physiological Reviews, 59: 606-718.

- Clark, A.M., 1996. Natural products as a source for new drugs. Pharmaceutical Research, 13: 1133-1141.

- Coulson, F.R., Jacoby, D.B. and Fryer, A.D., 2004. Insulin regulates neuronal M₂ muscarinic receptor function in the ileum of diabetic rats. The Journal of Pharmacology and Experimental Therapeutics, 308: 760-766.

- Dornberger, K., 1986. The potential antieoplastic acting constituents of Physalis alkekengi L. var franchetti Mast. Die Pharmazie, 41(4): 265-268.

- El Bardai, S., Hamaide, M.C., Lyoussi, B., Quetin-Leclercq, J., Morel, N. and Wibo, M., 2004. Marrubenol interacts with the phenylalkylamine binding site of the L-type calcium channel. European Journal of Pharmacology, 492: 269-272.

- Fujimoto, S. and Mori, M., 2004. Characterization of capsaicin-induced, capsazepine-insensitive relaxation of ileal smooth muscle of rats. European Journal of Pharmacology, 487: 175-182.

- Gilani, A.H., Aziz, N., Khurram, I.M., Chaudhary, K.S. and Iqbal, A., 2001. Bronchodilator, spasmolytic and calcium antagonist activities of Nigella sativa seeds (Kalonji): a traditional herbal product with multiple medicinal uses. The Journal of the Pakistan Medical Association, 51: 115-120.

- Gray, A.C., White, P.J. and Coupar, I.M., 2005. Characterisation of opioid receptors involved in modulating circular and longitudinal muscle contraction in the rat ileum. British Journal of Pharmacology, 144: 687-694.

- Kanada, A., Hata, F., Suthamnatpong, N., Maehara, T., Ishii, T., Takeuchi, T. and Yagasaki, O., 1992. Key roles of nitric oxide and cyclic GMP in nonadrenergic and noncholinergic inhibition in rat ileum. European Journal of Pharmacology, 216: 287-292.

- Kawai, M., Yamamoto, T., Makino, B., Yamamura, H., Araki, S., Butsugan, Y. and Saito, K., 2001. The structure of physalin T from Physalis alkekengi var. franchetti. Journal of Asian Natural Products Research, 3(3): 199-205.

- Lebrun, F., Francois, A., Vergnet, M., Lebaron-Jacobs, L. Gourmelon, P. and Griffiths, N.M., 1998. Ionizing radiation stimulates muscarinic regulation of rat intestinal mucosal function. American Journal Physiology, Gastrointestinal Liver Physiology, 275(6): G1333-G1340.

- Madeira, S.V.F., Matos, F.J.A., Leal-Cardoso, J.H. and Criddle, D.N., 2002. Relaxant effects of the essential oil of Ocimum gratissiumum on isolated ileum of the guinea pig. Journal of Ethnopharmacology, 81: 1-4.

- Pacaud, P., Feolde, E., Frelin, C. and Loirand, G., 1996. Characterization of the P2Y-purinoceptor involved in the ATP-induced rise in Cytosolic Ca²⁺ concentration in rat ileal myocytes. British Journal of Pharmacology, 118: 2213-2219.

- Pintea, A., Varga, A., Stepnowski, P., Socaciu, C., Culea, M. and Diehl, H.A., 2005. Chromatographic analysis of carotenol fatty acid esters in Physalis alkekengi and Hippophae rhamnoides. Phytochemical Analysis, 16(3): 188-195.

- van der Vliet, A., Rademaker, B. and Bast, A., 1990. A beta adrenoceptor with atypical characteristics is involved in the relaxation of the rat small intestine. The Journal of Pharmacology Experimental Therapeutics, 255: 218-226.

- Vessal, M., Fathi, N. and Khoshdel, Z., 2004. Effect of aqueous extract of Physalis alkekengi fruits on the activity of ovarian 3 beta-and 20alpha-hydroxysteroid dehydrogenases in late pregnancy in rat. Iranian Journal of Medical Sciences, 29(4): 175-179.

- Vessal, M., Mehrani, H.A. and Omrani, G.H., 1991. Effect of an aqueous extract of Physalis alkekengi fruits on estrus cycle, reproduction and uterine creatine kinase BB-isozyme in rats. Journal of Ethnopharmacology, 34: 69-78.

- Vessal, M. and Yazdanian, M., 1995. Comparison of the effects of an aqueous extract of Physalis alkekengi fruits and/or various doses of 17-beta-estradiol on rat estrous cycle and uterine glucose 6-phosphate dehydrogenase activity. Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology, 112(2): 229-236.

- Weller, P. and Breithaupt, D.E., 2003. Identification and quantification of zeaxanthin esters in plants using liquid chromatography-mass spectrometry. Journal of Agricultural and Food Chemistry, 51(24): 7044-7049.

- Yegnanarayan, R. and Shrotri, D.S., 1982. Comparison of antidiarrheal activity of some drugs in experimental diarrhea. Indian Journal of Pharmacology, 14: 293-299.

- Zhang, W.W., Li, Y., Wang, X.Q., Tian, F., Cao, H., Wang, M.W. and Sun, Q.S., 2005. Effects of magnolol and honokiol derived from traditional Chinese herbal remedies on gastrointestinal movement. World Journal of Gastroenterology, 11: 4414-4418.

Iranian Journal of Medicinal and Aromatic Plants Research

Antispasmodic activity of Physalis alkekengi L. leaf hydroalcoholic extract on rat ileum

References

References

Volume 23, Issue 3 - Serial Number 37
November 2007
Pages 340-349

Antispasmodic activity of Physalis alkekengi L. leaf hydroalcoholic extract on rat ileum

References

References

Volume 23, Issue 3 - Serial Number 37November 2007Pages 340-349

Volume 23, Issue 3 - Serial Number 37
November 2007
Pages 340-349