Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran

Valizadeh, M.; Bagheri, A.; Valizadeh, J.; Mirjalili, M.H.; Moshtaghi, N.

doi:10.22092/ijmapr.2015.101792

Document Type : Research Paper

Authors

¹ Department of Biotechnology and Plant Breeding, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

² Department of Biology, College of Science, University of Sistan and Baluchistan, Zahedan, Iran

³ Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

https://doi.org/10.22092/ijmapr.2015.101792

Abstract

Withania coagulans (Stocks) Dunal (fam. Solanaceae) is a multipurpose medicinal plant, mainly distributed in southeastern parts of Sistan and Baluchestan province. The important medicinal properties of W. coagulans are attributed to the presence of steroidal lactones called withanolides. Among withanoloids, withaferin A is an important phytoconstituent showing antitumor, antiangiogenesis and apoptosis induction properties. This study was aimed to investigate the variability of phytochemical composition of Withania coagulans roots. Twenty accessions of W. coagulans roots, collected from different natural habits of Sistan and Baluchestan province, were used in the present study. Thewithaferin A was assayed through thin layer chromatography (TLC) method andphytochemical estimation was carried out by standard methodologies to detect the presence of secondary metabolites, like total phenol, flavonoids and anthocyanin. Our findings revealed that withaferin A was found in all accessions collected from different natural habits. The photochemical investigation showed the presence of flavonoids (5.70-6.50%), anthocyanin (4.51-9.51µmol/g) and total phenol (14.91-23.7µgGAE/mgD.W), varying significantly among the habitats (p<0.05). In the present study, TLC analysis confirms the exsistance of withaferin A in all W. coagulans accessions as well as the potential of Sistan and Baluchestan natural habits to produce this valuable component. However, the root extracts of W. coagulans, growing in natural habitats of Iran, have higher phenolic content. Hence, it can be used as a source of natural components for the pharmaceutical and food industries.

Keywords

References

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Iranian Journal of Medicinal and Aromatic Plants Research

Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran

References

References

Volume 31, Issue 3 - Serial Number 71
August 2015
Pages 406-417

Phytochemical investigation of Withania coagulans (Stocks) Dunal in natural habits of Sistan and Baluchestan province of Iran

References

References

Volume 31, Issue 3 - Serial Number 71August 2015Pages 406-417

Volume 31, Issue 3 - Serial Number 71
August 2015
Pages 406-417