Document Type : Research Paper
Authors
1 Azad University of Science and Technology, Tehran
2 Research Institute of Forest and Rangelands, Tehran
3 Agricultural College, University of Tehran, Karaj
Abstract
Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 13 Iranian landraces of Ferula gummosa Boiss. The 7 primers used in this study amplified 69 scorable RAPD bands among which 64 were polymorphic (94%). The average number of bands was 9.14 for each primer. Dice similarity index was used for measuring genetic similarities among landraces. The highest similarity (0.80) was found between Hamlon and Reef populations, whereas the lowest was between Hamloon and Semnan. UPGMA algorithm was used for cluster analysis. Cluster analysis separated the 13 landraces into three main groups. The results indicated that RAPD technique is an efficient tool for assessing genetic diversity in Ferula gummosa populations.
Keywords