Agriculture and horticulture
M. Nosrati Momvandi; E. Zeid Ali; M.J. Zarea; H. Mumivand; M. Kiani
Abstract
To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, ...
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To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, and carvacrol percentage) of Satureja rechingeri Jamzad, a factorial experiment was conducted in a randomized complete block design with three replications in 2017. Based on the results, all the mentioned traits except the stems to leaves ratio were affected by planting date and full fertilizer treatment. The planting date of April 21 and the application of full fertilizer increased all the studied traits except the carvacrol percentage and the stems to leaves ratio. The morning harvest time treatment increased the percentage and essential oil yield. The highest percentage of carvacrol (93.66%) was obtained in the triple interaction of planting date (March 20) × full fertilizer (two per thousand) × harvest time (evening) and the lowest one (82.77%) was obtained in the triple interaction of planting date (March 20) × no fertilizer application × harvest time (morning). The results showed that in the planting date of April 21 treatment, the application of full fertilizer caused better results in most of the mentioned traits. Also, the morning and evening harvest treatments caused the highest percentage and essential oil yield and carvacrol percentage. To investigate the effects of planting date (March 20 and April 21), full fertilizer (PLANTFEED) (zero and two per thousand), and harvest time (8 am, 12 noon, and 19 pm) on the quantitative and qualitative yield (leaves and stems dry weight, stems to leaves ratio, yield and percentage of essential oil, and carvacrol percentage) of Satureja rechingeri Jamzad, a factorial experiment was conducted in a randomized complete block design with three replications in 2017. Based on the results, all the mentioned traits except the stems to leaves ratio were affected by planting date and full fertilizer treatment. The planting date of April 21 and the application of full fertilizer increased all the studied traits except the carvacrol percentage and the stems to leaves ratio. The morning harvest time treatment increased the percentage and essential oil yield. The highest percentage of carvacrol (93.66%) was obtained in the triple interaction of planting date (March 20) × full fertilizer (two per thousand) × harvest time (evening) and the lowest one (82.77%) was obtained in the triple interaction of planting date (March 20) × no fertilizer application × harvest time (morning). The results showed that in the planting date of April 21 treatment, the application of full fertilizer caused better results in most of the mentioned traits. Also, the morning and evening harvest treatments caused the highest percentage and essential oil yield and carvacrol percentage.
Agriculture and horticulture
B. Andalibi; M. Mohammadi Azar; B. Esmailpour; F. Shekari
Abstract
This study was conducted to investigate the effects of salicylic acid (SA) and nanosilicon (n-Si) on some morphophysiological characteristics and essential oil of Lallemantia iberica under salinity stress and uncontrolled greenhouse conditions as a factorial experiment based on the randomized complete ...
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This study was conducted to investigate the effects of salicylic acid (SA) and nanosilicon (n-Si) on some morphophysiological characteristics and essential oil of Lallemantia iberica under salinity stress and uncontrolled greenhouse conditions as a factorial experiment based on the randomized complete block design in 2018. The experimental factors included sodium chloride (0, 50, and 100 mM), SA (0, 1, and 2 mM), and n-Si (0, 0.5, and 5 mM). The SA and n-Si treatments were applied at the four-leaf stage (once every seven days) and the salinity treatment was applied at the six-leaf stage of the plant (once every four days) until fully ripening seeds of the plant (yellowing 90% of the leaves and capsules). The results showed that under salinity stress, the plant traits including height, aerial parts dry weight, chlorophyll content, and seed yield decreased and proline and essential oil increased. The application of SA and n-Si improved these traits under salinity stress. The main constituents of L. iberica essential oil included linalool (25%), geraniol (16%), β-cubebene (10.6%), menthyl acetate (9.8%), valencene (3.5%), germacrene-D (2.4%), β-caryophyllene (2.3%), and limonene (1.3%), all of which increased in the salinity treatments compared to the control. The application of SA and n-Si under salinity stress conditions increased limonene, linalool, geraniol, β-cubebene, β-caryophyllene, and valencene and decreased menthyl acetate and germacrene D. In general, the results of this study showed that the application of SA and n-Si could increase the tolerance of L. iberica to salinity stress by creating the osmotic regulation, protecting the content of photosynthetic pigments, and reducing oxidant damage.
Agriculture and horticulture
A. Rahmani; E. Sharifi Ashourabadi; A. Ebrahimi; M. Mirza
Abstract
To investigate the effects of soil fertility methods on the yield and yield components of hyssop (Hyssopus officinalis L.) in the different harvests, an experiment was conducted as a factorial in a randomized complete block design with three replications in Karaj (Alborz province) in 2017. The experimental ...
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To investigate the effects of soil fertility methods on the yield and yield components of hyssop (Hyssopus officinalis L.) in the different harvests, an experiment was conducted as a factorial in a randomized complete block design with three replications in Karaj (Alborz province) in 2017. The experimental treatments included the harvest time and soil fertility methods. The results showed that the first harvest had higher plant height, number of sub-branches, and leaf area index and the second harvest had higher plant dry matter weight and chlorophyll content. With the soil fertility, the plant height, number of sub-branches, plant dry matter weight, leaf area index, chlorophyll content, essential oil yield, and nutrients uptake increased significantly. The highest plant height and number of sub-branches was obtained from the application of 120 kg ha-1 nitrogen+ 96 kg ha-1 phosphorus+ 120 kg ha-1 potassium treatment. The maximum dry matter weight of the plant was allocated to the combination of 20 t ha-1 manure with 80 kg ha-1 nitrogen+ 64 kg ha-1 phosphorus+ 80 kg ha-1 potassium. The highest essential oil yield was obtained from the 120 kg ha-1 nitrogen+ 96 kg ha-1 phosphorus+ 120 kg ha-1 potassium treatment which was statistically in the same group as the treatments including 40 t ha-1 manure, combination of 30 t ha-1 manure with mycorrhiza, and combination of 40 kg ha-1 nitrogen+ 32 kg ha-1 phosphorus+ 40 kg ha-1 potassium with 30 t ha-1 manure. Based on the results, the desired quantitative and qualitative yield in hyssop could be achieved with the integrated feeding method and use of biological and organic fertilizers.
Agriculture and horticulture
M. Babaei; F. Sefidkon; M. Nasiri
Abstract
Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete ...
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Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete block design in Alborz Research Station of Research Institute of Forests and Rangelands (Karaj, Alborz province). The flowering branches were harvested at the full flowering stage and after room temperature-drying, their essential oils were extracted by hydrodistillation, and analyzed and identified by GC and GC/MS. The essential oil yield of N. cataria populations varied between 0.02 (Karaj) and 0.50% (Arak). Twenty-three compounds were identified in the essential oil of this species, and the main compound in all populations was from nepetalactone isomers. NepetalactoneIII (4aα,7β,7aα-nepetalactone) and nepetalactoneI (4aα,7α,7aα-nepetalactone) constituted 44.4 (Karaj) to 91.6% (Arak) and 0.8 (Karaj) to 15.9% (Bafgh1) of the essential oil, respectively. NepetalactoneII (4aα,7α,7aβ-nepetalactone) was observed only in the population Bafgh2 (21.2%). The amount of 1,8-cineole in the essential oil of different N. cataria populations varied from 0.4 (Taft1) to 12.8% (Karaj). The essential oil yield of N. bractaeta populations was obtained between 0.02 (Ardakan) and 0.70% (Taft2). Twenty-seven compounds were identified in the essential oil of this species, and the main compounds were 1,8-cineole (1.0, 9.6, and 41.0% in Tabas, Ardakan, and Taft2, respectively) and geranyl acetate (0.9, 3.4, and 39.8% in Tabas, Taft2, and Ardakan, respectively). In general, the results showed that the N. cataria populations were all from the same chemotype, but N. bracteata populations were from two chemotypes (ct. geranyl acetate and ct. 1,8-cineole).
Phytochemistry (extraction, identification and measurement of active components)
V. Abdossi; B. Tavakoli; A. Mehrafarin; H.A. Naghdibadi
Abstract
To evaluate the quantitative and qualitative characteristics of Echinophora platyloba DC. under the influence of different drying methods, an experiment was conducted in a completely randomized design with 12 treatments and three replications in 2019. The experimental treatments included fresh plant, ...
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To evaluate the quantitative and qualitative characteristics of Echinophora platyloba DC. under the influence of different drying methods, an experiment was conducted in a completely randomized design with 12 treatments and three replications in 2019. The experimental treatments included fresh plant, drying the plant in the shade and sun, drying the plant with an oven at three temperatures of 45, 55, and 65 °C, drying the plant with a vacuum oven at three temperatures of 45, 55, and 65 °C, and drying the plant with a microwave at three powers of 200, 400, and 600 W. The aerial parts of the plant was collected from the heights of Tuyserkan city in Hamedan province in June and was affected by the different drying methods. The essential oil was extracted by Clevenger apparatus and then its components were identified by GC-MS. The findings of this study showed that the different methods of plant drying had a significant effect on the percentage and all the components of essential oil. The microwave drying method at 400 W, despite not being suitable for preserving the secondary metabolites of the plant, caused the highest essential oil content. The shade-drying method, despite the long drying time, was the most suitable method for preserving the essential oil composition in E. platyloba. The essential oil percentage in this study ranged from 0.06% to 0.39% and the main essential oil constituents included trans-β-ocimene (34.16-57.52%), α-phellandrene (8.87-16.52%), cis-ocimene (1.69-4.57%), b-phellandrene (2.08-9.24%), p < /em>-cymene (1.53-5.05%), β-pinene (0.73-1.94%), a-pinene (1.83-5.15%), veloutone (0.39-3.78%), trans-carveol (0.38-1.92%), linalool (0.72-3.57%), and germacrene-D (1.67-4.91%).
Agriculture and horticulture
S.F. Hosseini; J. Hamzei
Abstract
To investigate the effects of common bean (Phaseolus vulgaris) and its residuals on agromorphological traits, yield, and qualitative characteristics of dragonhead (Dracocephalum kotschyi Boiss.) under intercropping conditions, and finally to evaluate the usefulness of intercropping compared to the monoculture ...
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To investigate the effects of common bean (Phaseolus vulgaris) and its residuals on agromorphological traits, yield, and qualitative characteristics of dragonhead (Dracocephalum kotschyi Boiss.) under intercropping conditions, and finally to evaluate the usefulness of intercropping compared to the monoculture of dragonhead, an experiment was conducted in a randomized complete blocks design with three replications at the research farm of Bu-Ali Sina University in Hamedan during growing seasons of 2018 and 2019. Experimental treatments included the additive intercropping of 0, 20 and 40% bean with dragonhead. The bean residuals were remained at the farm in the first year to determine its effects on quantitative and qualitative yield of dragonhead in the second year. Based on the results, the intercropping improved the agromorphological traits and yield of dragonhead compared to the control (dragonhead monoculture). The results showed that in the intercropping of 20% bean, the percentage and yield of essential oil, the phenol content, and the antioxidant percentage of dragonhead were higher than the control treatment, but flavonoids content was higher in the control. The results in the study of effects of bean residuals on the quantitative and qualitative traits of dragonhead in the second year showed that the meet of dragonhead needs in terms of nutrients like nitrogen increased its quantitative and qualitative yield compared to the control treatment. The relative yield of dragonhead was higher than bean, which indicated the profitability of dragonhead from intercropping with bean. In fact, the indices of land equivalent ratio (LER), competitive ratio (CR), and aggressivity (AG) showed the usefulness of dragonhead intercropping. Overall, the additive intercropping of 20% bean with dragonhead was identified as the superior treatment and can be used by farmers for organic production of dragonhead.
Improvement and breeding
H. Hasan-beigi; M. Saidi; M. Mohammadi
Abstract
To investigate the effects of foliar application of gibberellic acid (GA3) and salicylic acid (SA) on the morphophysiological, biochemical, and yield characteristics of Echinacea purpurea (L.) Moench, an experiment began as a factorial in a randomized complete blocks design with three replications at ...
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To investigate the effects of foliar application of gibberellic acid (GA3) and salicylic acid (SA) on the morphophysiological, biochemical, and yield characteristics of Echinacea purpurea (L.) Moench, an experiment began as a factorial in a randomized complete blocks design with three replications at the research farm of Agriculture Faculty, Ilam University, Iran, by sowing the plants in 2018 and continued by evaluating the plant characteristics for the two consecutive years (2018 & 2019). The experimental treatments included the foliar spray of plants during vegetative growth period with GA3 (0, 100, and 200 ppm) and SA (0, 100, and 200 ppm) as well as their combinations. The results showed that the highest plant height, number of flowers, and fresh and dry weight of the plants (in both years) were obtained in the GA3-200 ppm treatment and the highest amount of total chlorophyll and carotenoids (in both years) in the GA3-100 ppm one. The highest capitol diameter and fresh and dry weight of flowers in the first year and the highest anthocyanins content in the second year were observed in the GA3-200 ppm treatment. Also, the highest capitol diameter in the second year and the highest anthocyanins content in the first year were obtained in the SA-100 ppm treatment. In addition, the highest fresh and dry weight of flowers in the second year was recorded in the SA-200 ppm treatment. The highest essential oil amount of flowers, shoots (without flowers), and total essential oil (in both years) were obtained in the GA3-100 ppm+SA-100 ppm combination treatment. Considering that increasing the plant dry matter yield as well as essential oil content were the most important objectives of this study, the GA3-200 ppm treatment for the plant dry weight increase and the GA3-100 ppm+SA-100 ppm combination treatment for the essential oil content increase could be suggested as the best treatments.
Improvement and breeding
J. Gheshlaghpour; R. Khademian; B. Asghari; B. Sedaghati
Abstract
To evaluate the silicon effects on morphological characteristics as well as the percentage and composition of basil (Ocimum basilicum L.) essential oil under cadmium stress, a factorial greenhouse experiment was conducted in a completely randomized design. The treatments included cadmium metal (Cd) as ...
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To evaluate the silicon effects on morphological characteristics as well as the percentage and composition of basil (Ocimum basilicum L.) essential oil under cadmium stress, a factorial greenhouse experiment was conducted in a completely randomized design. The treatments included cadmium metal (Cd) as the first factor at three levels of 0, 25, and 50 mg kg-1 soil and silicon (Si) as the second factor with concentrations of 0, 1, and 2 mM. The characteristics including root length, fresh and dry weight of roots, plant height, fresh and dry weight of stems, stem diameter, number of leaves, fresh weight of leaves, number of buds, number of sub-stems, and percentage and composition of essential oil were evaluated. The results showed that Cd had negative effects on all studied morphological characteristics and significantly decreased their values compared to the control. However, Si treatment improved all these traits and with increasing the concentration of this element, a significant increase in the values of these traits was observed. Application of Si in the concentration of 2 mM caused 63.8, 81, 61, and 61% increase in the number of sub-stems, root fresh weight, stem fresh weight, and number of buds compared to the control, respectively. The amounts of five major constituents of basil essential oil including 1,8-cineole, linalool, estragole, methyl eugenol, and eugenol were affected significantly with the Cd and Si treatments. However, the interaction of these two treatments showed a significant effect only on the three compounds 1,8-cineole, linalool, and methyl eugenol. The maximum amounts of 1,8-cineole (5.06%) and methyl eugenol (6.21%) were observed in the plants which simultaneously exposed to the highest concentrations of Cd and Si. The amount of linalool was also decreased with the presence of Cd and increased with the Si treatment. The Cd treatment increased the amount of estragole by 12.8% and decreased the amount of eugenol by 25.2%. The Si treatment also increased the amount of estragole by 23.9% and decreased the amount of eugenol by 18.3%.
Phytochemistry (extraction, identification and measurement of active components)
A. Mahmoudi; M. Karami; M.T. Ebadi; M. Ayyari
Abstract
To evaluate the effect of infrared drying on color parameters, essential oil content and composition of German chamomile (Matricaria chamomilla L.), three different infrared intensities (125, 132, and 220 W) with three airflow velocities (0.5, 1.0, and 1.5 m.s-1) on a total of nine treatments were ...
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To evaluate the effect of infrared drying on color parameters, essential oil content and composition of German chamomile (Matricaria chamomilla L.), three different infrared intensities (125, 132, and 220 W) with three airflow velocities (0.5, 1.0, and 1.5 m.s-1) on a total of nine treatments were examined in a completely randomized design, and the shade-dried plants were considered as a control treatment. The results indicated that the treatments had significant effects on drying time, color parameters, and essential oil content. The control (shade drying) and the 220 W radiation intensity with 0.5 m.s-1 air velocity treatments had the highest (2367 min) and lowest (83 min) drying time, respectively. With increasing infrared intensity, the main color parameters such as L, a, b, ΔE (total color changes) and BI (browning index) changed significantly, compared to the control; however, increasing the airflow rate caused less variation of the mentioned parameters in all levels of infrared radiation intensity than the control. The highest essential oil content (0.23% w/w) was obtained at 125 W radiation intensity with 0.5 m.s-1 air velocity and the lowest (0.12% w/w) was observed at the 220 W radiation intensity with 0.5 and 1 m.s-1 air velocity treatments. Essential oil analysis showed that the highest amount of α-bisabolol-oxide A (60.6%), (Z)-spiroether (16.5%), and chamazulene (4.6%) belonged to 132 W radiation intensity with 0.5 m.s-1 air velocity, 220 W radiation intensity with 0.5 m.s-1 air velocity, and control treatments, respectively. In general, the results of this experiment showed that due to the decreased duration of drying time, color preservation and desired essential oil content and composition, the 125 W radiation intensity with 0.5 m.s-1 air velocity treatment could be a suitable method for chamomile flowers drying.
Z. Heydari; A. Yavari; L. Jafari; H. Mumivand
Abstract
Salvia sharifii Rech. f. & Esfand. is an endemic medicinal plant in Iran and belongs to the Lamiaceae family. In the current study, different plant parts (flower, leaf, and stem) of this species were collected from the Bokhon region of Hormozgan province in March 2018 and were studied in terms of ...
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Salvia sharifii Rech. f. & Esfand. is an endemic medicinal plant in Iran and belongs to the Lamiaceae family. In the current study, different plant parts (flower, leaf, and stem) of this species were collected from the Bokhon region of Hormozgan province in March 2018 and were studied in terms of the amount of essential oil and variability in their composition. The essential oils of air-dried samples were extracted by hydro-distillation and analyzed by GC and GC-MS. The essential oil yield of flower, leaf, and stem was obtained 1.38, 1.14, and 0.84% (w/w), respectively. The total number of essential oil compounds identified and quantified was 45 in flower, 42 in leaf, and 43 in the stem. The results of essential oil compounds analysis revealed that linalool (38.7%), hexyl isovalerate (13.8%), hexyl caprylate (6.2%), hexyl isobutyrate (4.6%), hexyl-2-methyl butyrate (4.4%), trans-caryophyllene (3.8%), and n-hexyl hexanoate (3.7%) were the major compounds in flower. In leaf, linalool (17.0%), hexyl caprylate (11.1%), α-humulene (7.8%), trans-caryophyllene (6.2%), sclareol oxide (5.9%), nootkatone (3.7%), hexyl isovalerate (3.5%) and agarospirol (3.0%) had the highest amounts in essential oil. In stem essential oil, linalool (18.7%), sclareol oxide (8.0%), α-humulene (7.5%), trans-caryophyllene (7.2%), hexyl isovalerate (5.2%), caryophyllene oxide (5.0%), n-hexyl hexanoate (4.8%), nootkatone (4.1%) and dibutyl phthalate (4.0%) were the major compounds. The major and common compound in the different organs essential oil of this species was linalool which was the highest in flowers and the lowest in leaves. The presence of chemical diversity in the essential oil of different organs of this species can be considered by the pharmaceutical, food, and cosmetic industries, as well as medicinal plant breeders in selecting the appropriate organ for consumption and breeding purposes.
A. Abdollahi Arpanahi; M. Feizian; Gh. Mehdipourian
Abstract
To investigate the effects of plant growth promoting rhizobacteria (PGPR) and drought stress on essential oil of Thymus daenensis Clack, a greenhouse experiment was conducted in Shahrekord, Iran in 2017. The experiment was arranged as a factorial in a completely randomized design with three replications. ...
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To investigate the effects of plant growth promoting rhizobacteria (PGPR) and drought stress on essential oil of Thymus daenensis Clack, a greenhouse experiment was conducted in Shahrekord, Iran in 2017. The experiment was arranged as a factorial in a completely randomized design with three replications. The first factor included four levels of irrigation: well-watered (No stress), irrigation after depletion of 20-25% of field capacity (FC) (Low stress), irrigation after depletion of 35-40% of FC (Mild stress) and irrigation after depletion of 55-60% of FC (Severe stress). The second factor included two levels of PGPR treatments: no inoculation (Control) and inoculation with PGPR. The results showed that the morphological parameters were significantly increased in PGPR treatments, while water stress decreased all parameters. The essential oil amount increased in low stress and decreased in severe stress. PGPR inoculation increased the amount of essential oil, although this increase was not statistically significant. PGPR incubation and drought stress had a significant effect on 13 and 14 components, respectively. The interaction of PGPR and drought stress had a significant effect on the oil components. Thymol and carvacrol, two important components of T. daenensis essential oil, decreased with increasing severity of drought stress, but PGPR inoculation increased them, especially at drought stress treatments.
A. Mahmudi; M.T. Ebadi; M. Ayyari
Abstract
In this study, Eryngium thyrsoideum Boiss. was collected from three regions Razjerd (Rz) (Qazvin province), Salavatabad (Sv) (Sanandaj, Kurdistan province), and Paygelan (Py) (Marivan, Kurdistan province) at 1700, 1780, and 1840 m above the sea level, respectively, and ...
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In this study, Eryngium thyrsoideum Boiss. was collected from three regions Razjerd (Rz) (Qazvin province), Salavatabad (Sv) (Sanandaj, Kurdistan province), and Paygelan (Py) (Marivan, Kurdistan province) at 1700, 1780, and 1840 m above the sea level, respectively, and their volatile components were evaluated. The shade-dried plant materials were hydrodistillated by Clevenger-type apparatus for three hours. The analysis of essential oils composition was carried out by gas chromatography-mass spectrometry (GC-MS) and quantified by GC-FID. The essential oil content was obtained 0.06, 0.05, and 0.07% (w/w) for Rz, Py and Sv, respectively. The main components of essential oils were 2,3,6-trimethyl benzaldehyde (18.6, 11.5 and 34.0%), 2,4,6-trimethyl benzaldehyde (3.0, 1.0 and 4.1%), germacrene D (2.5, 12.6 and 6.0%), ethyl linoleate (7.6, 8.7 and 5.1%), sesquicineole (0.3, 5.8 and 3.0%), and trans-caryophyllene (5.6, 2.5 and 1.9%) (the numbers in parentheses for Rz, Py and Sv, respectively). In this regard, the water-soluble volatile components of these plants, as distilled water components obtained from the Clevenger side part, were also extracted and finally separated using ethyl acetate solvent and analyzed similarly to essential oil compounds. The amount of these components, as the organic fraction (Of) of distilled water, was 0.017, 0.02, and 0.04 gram per 100 ml of distilled water in Rz, Py, and Sv samples, respectively. The main identified components of these fractions were, 2,3,6-trimethyl-benzaldehyde (69.8, 54.5 and 58.6%), p-vinyl-guaiacol (12.0, 26.7 and 24.7%), 2,4,6-trimethyl-benzaldehyde (9.7, 5.8 and 4.4%), and trans-anethole (4.0, 4.1 and 5.0%) for OfRz, OfPy, and OfSv, respectively. Although E. thyrsoideum does not have a high essential oil percentage, it has a wide range of components. This species has a high content of trimethyl-benzaldehye family compounds. To the best of our knowledge, this is the first report of volatile components of E. thyrsoideum.
A. Javanmard; P. Karimi Karamjavan; M. Amani Machiani; A. Ostadi; S. Shahbazi Doorbash
Abstract
In order to investigate the effects of combined and separate application of macro and micro nano fertilizers with chemical fertilizers on quantitative and qualitative traits of medicinal plant dragon’s head (Lallemantia iberica (MB) Fischer & Meyer), a field experiment was performed based on ...
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In order to investigate the effects of combined and separate application of macro and micro nano fertilizers with chemical fertilizers on quantitative and qualitative traits of medicinal plant dragon’s head (Lallemantia iberica (MB) Fischer & Meyer), a field experiment was performed based on the randomized complete block design (RCBD) with seven treatments and three replications on the research farm of Maragheh University in 2017. Experimental treatments included the use of 100% conventional chemical fertilizer (urea and triple superphosphate with values of 150 and 100 kg ha-1, respectively), 100% macro nano fertilizer, 100% micro nano fertilizer, 50% macro nanofertilizer+50% micro nano fertilizer, 50% chemical fertilizer+50% macro nano fertilizer, 50% chemical fertilizer+50% micro nano fertilizer, and 50% chemical fertilizer+25% macro nanofertilizer+25% micro nano fertilizer. The results showed that the highest (73.3) and lowest (59.1) chlorophyll indices were obtained by applying 50% chemical fertilizer+25% macro nano fertilizer+25% micro nano fertilizer, and separate application of 100% micro nano fertilizer, respectively. Also, the highest values of number of lateral branches, main stem length, number of flower cycles per plant, number of seeds per plant, biological yield, seed yield, 1000-seed weight and seed mucilage content were obtained in 50% chemical fertilizer+25% macro nanofertilizer+25% micro nanofertilizer treatment. Chemical analysis of the essential oils demonstrated that linalool, menthol, geraniol, geranial, menthyl acetate, β-bourbonene, geranyl acetate, (E)-caryophyllene, germacrene D, spathulenol and caryophyllene oxide were the main constituents of essential oils. The highest content of β-bourbonene, caryophyllene oxide, (E)-caryophyllene, menthol, and geraniol was obtained with the combined use of 50% chemical fertilizer+25% macro nanofertilizer+25% micro nano fertilizer. In general, based on the results of the present research, the combined use of chemical fertilizers and macro and micro nano fertilizers can be recommended due to the increased seed yield and dragon’s head qualitative traits under rainfed conditions.
Z. Asle Mohammadi; N. Mohammadkhani; M. Servati
Abstract
The deficiency of nutrients such as nitrogen disrupts growth and changes the quantity and quality of essential oil. Foliar application of micronutrients improves plant growth. Therefore, due to this issue and its importance, the present study aimed to evaluate the effects of iron and zinc foliar spray ...
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The deficiency of nutrients such as nitrogen disrupts growth and changes the quantity and quality of essential oil. Foliar application of micronutrients improves plant growth. Therefore, due to this issue and its importance, the present study aimed to evaluate the effects of iron and zinc foliar spray on growth factors, percentage and essential oil components of thyme (Thymus vulgaris L.) under soil nitrogen deficiency. This study was conducted as a factorial experiment in a completely randomized design with three replications. Experimental treatments consisted of nitrogen levels (control and deficiency) and iron and zinc foliar spray (1% and 2%). The results showed that the essential oil yield of thyme decreased under nitrogen deficiency; however, the foliar application of micronutrients, especially zinc, significantly increased the essential oil yield. Also, thymol, γ-terpinene and p-cymene formed the most components of the essential oil. Thymol content increased in control plants (full strength Hoagland) with foliar spray and its highest amount (54.4%) was obtained with foliar spray at zinc-2%. The p-cymene content increased from 17% at nitrogen control level to 24.6% under nitrogen deficiency. In control plants (full strength Hoagland), foliar spray increased shoot length, and iron-2% increased shoot dry weight. The foliar application of micronutrients caused changes in the content of essential oil compounds and the synthesis of some new compounds such as 1,8-cineole in the essential oil. The foliar application also improved some growth factors such as shoot length and dry weight in the control plants.
S. Binava; A. Yavari; M. Shokrpour
Abstract
Salvia mirzayanii Rech. F. & Esfand. is one of the medicinal species of the Lamiaceae family, growing wild only in Iran. In this study, different plant organs (leaf, flower, stem and whole plant (=aerial parts)) were collected from the Khonj region of Fars province in March 2018 and studied for their ...
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Salvia mirzayanii Rech. F. & Esfand. is one of the medicinal species of the Lamiaceae family, growing wild only in Iran. In this study, different plant organs (leaf, flower, stem and whole plant (=aerial parts)) were collected from the Khonj region of Fars province in March 2018 and studied for their essential oil content and chemical composition. The essential oil of samples was extracted by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The essential oil yield of leaf, flower, stem and aerial parts was obtained to be 3.2, 2.6, 0.4 and 2.3% (w/w), respectively. The highest number of identified chemical components was observed in aerial parts and flowers (26 compounds) and the least in stem (23 compounds). The results of essential oil compound analysis revealed that α-terpinyl acetate, linalool, 1,8-cineol, β-eudesmol, δ-cadinene, and α-terpineol were the main compounds in the leaf essential oil. The main compounds of flower essential oil were included δ-cadinene, α-terpinyl acetate, linalool, β-eudesmol, and γ-cadinene. Compounds α-terpinyl acetate, linalool, linalool acetate, 1,8-cineol, and α-terpineol were abundantly found in the stem essential oil. The main compounds in the essential oil of aerial parts were δ-cadinene, α-terpinyl acetate, linalool, 1,8-cineol, γ-cadinene, and α-terpineol. The presence of chemical diversity in the essential oil of the whole plant and its various organs could be considered for pharmaceutical, food and cosmetic industries as well as for the plant breeders in selecting the appropriate organs for consumption and breeding purposes.
M. Bromand Sivieri; M. Heidary; A. Gholami; H. Ghorbani
Abstract
In order to study the effects of nano iron oxide and different biofertilizers on the quantitative and qualitative yield of black cumin (Nigella sativa L.), a factorial experiment based on randomized complete block design with three replications was conducted in the research field of Shahrood Industrial ...
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In order to study the effects of nano iron oxide and different biofertilizers on the quantitative and qualitative yield of black cumin (Nigella sativa L.), a factorial experiment based on randomized complete block design with three replications was conducted in the research field of Shahrood Industrial University in 2017 growing season. Experimental treatments included the foliar spray of nano iron oxide at three levels of 0, 1.5 and 3 g L-1 as the first factor and five levels of biofertilizer including no biofertilizer (control), Glomus intraradices, G. mosseae, Azospirillum, and Azotobacter as the second factor. The results of this experiment showed that the application of fungal and bacterial treatments significantly increased the grain yield and yield components as well as the concentration of nitrogen and iron in seed, and also had a significant effect on increasing essential oil content, thymoquinone content of the essential oil, and seed oil. The most effective treatment was G. intraradices, which was able to significantly improve all traits measured. The foliar application of nano iron oxide had a significant and positive effect only on the essential oil content, biological yield, and iron and nitrogen content of the seed. The highest amount of iron and nitrogen content of the seed was obtained in nano iron oxide treatment at 3 g L-1 level. However, the essential oil content, essential oil thymoquinone percentage, seed oil content, and also biological yield did not differ significantly between the two concentrations of nano iron oxide. Therefore, it seems that the application of a lower concentration of nano iron oxide (1.5 g L-1) together with G. intraradices can improve the quantitative and qualitative yield in the medicinal plant black cumin by saving fertilizer consumption without a significant decrease in the qualitative traits measured.
M. Babashpour-Asl; M. Piryaei; I. Farhang
Abstract
The genus Artemisia comprises a large number of species, some of which are cultivated commercially in a variety of applications. The high accumulation of essential oils and other terpenoids in certain members of family Asteraceae, such as wormwood (Artemisia absinthium L.), has made them useful as flavorings. ...
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The genus Artemisia comprises a large number of species, some of which are cultivated commercially in a variety of applications. The high accumulation of essential oils and other terpenoids in certain members of family Asteraceae, such as wormwood (Artemisia absinthium L.), has made them useful as flavorings. Terpenoids and phenolic compounds are also responsible for the medicinal and medical values of many members of this family. The solid-phase microextraction (SPME) is a fast, easy, efficient, highly solvent-free, and easy-to-automate method. In the present study, headspace SPME (HS-SPME) using nanostructured layered double hydroxide coated fiber in combination with gas chromatography-mass spectrometry was utilized as a monitoring technique for the collection and detection of essential oil constituents of wormwood. Experimental parameters including temperature and desorption time, temperature and extraction time, sample weight, and water content effect were tested and optimized. A temperature of 260 °C was found to be appropriate for efficient desorption. The best desorption time was obtained 2 min for all measurements. The optimized quantities were found for extraction temperature 75 °C, extraction time 25 min, sample weight 2.5 g. The added water deactivated the fiber surface by blocking the active sites; therefore, the proposed fiber is suitable for dry samples. The proposed method is simple and costly compared to conventional methods of analysis. In addition, in this method, the analysis time is shorter and could be used for the analysis of volatile compounds in medicinal plants.
F. Jangi; M.T. Ebadi; M. Ayyari
Abstract
In order to investigate the effects of infrared radiation intensity and air velocity on some of the qualitative characteristics of hyssop (Hyssopus officinalis L.) during the drying process, a factorial experiment was conducted in a completely randomized design with three replications. The infrared radiation ...
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In order to investigate the effects of infrared radiation intensity and air velocity on some of the qualitative characteristics of hyssop (Hyssopus officinalis L.) during the drying process, a factorial experiment was conducted in a completely randomized design with three replications. The infrared radiation intensity was investigated at three levels of 0.2, 0.3 and 0.5 W/cm2 and the air flow velocity at 0.5, 1 and 1.5 m/s, and traits such as color parameters, essential oil content and composition were evaluated. The results showed a significant effect of treatments on all studied traits. In the radiation intensity of 0.3 W/cm2, with increasing air flow velocity, there was a significant increase in the desired color parameters such as brightness index (L), green color (a) and color purity (chroma). Generally, the color of samples was better maintained at lower radiation intensities. The essential oil content of dried samples in the intensity of 0.2 and 0.3 W/cm2 was about 0.2% higher than the radiation intensity of 0.5 W/cm2. Increasing the air flow velocity at low radiation intensities reduced the essential oil percentage, but in 0.5 W/cm2, the essential oil content increased by 0.17% with increasing the air flow velocity. The amount of cis-Pinocamphone in the essential oil of treated samples with a low-intensity infrared radiation was higher than the high-intensity treated ones, so that the amount of this component in 0.2 W/cm2 was 3.5 to 18.9% more than 0.5 W/cm2. Overall, the results of this study showed that the use of low infrared radiation intensity in drying process of hyssop could preserve leaves color parameters along with the essential oil content and its main components.
B. Bahreininejad; B. Abaszadeh; F. Sefidkon; Z. Jaberalansar
Abstract
Two species of Nepeta assurgens Hausskn. & Bornm. and N. eremokosmos Rech., are endemic medicinal plants to Iran. This study was conducted to investigate the vegetative traits, aerial parts yield, the quantity and quality of essential oil, as well as evaluating the relationships between these traits ...
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Two species of Nepeta assurgens Hausskn. & Bornm. and N. eremokosmos Rech., are endemic medicinal plants to Iran. This study was conducted to investigate the vegetative traits, aerial parts yield, the quantity and quality of essential oil, as well as evaluating the relationships between these traits in mentioned species under field conditions in Isfahan. The experiment was carried out in a randomized complete block design with three replications over 2017 and 2018. Results showed that the fresh and dry weight of aerial parts in N. assurgens (11353 and 2351 kg ha-1, respectively) was remarkably higher than that of N. eremokosmos (2638 and 527 kg ha-1, respectively). The average content and yield of essential oil in N. assurgens and N. eremokosmos was 1.60 and 0.64%, and 20.16 and 2.21 kg ha-1, respectively. Based on qualitative analysis of essential oil, the total amount of nepetalactone isomers (4aα, 7α, 7aα-nepetalactone, 4aα, 7α, 7aβ-nepetalactone, 4aα, 7β, 7aα-nepetalactone) in N. assurgens and N. eremokosmos was 78.6 and 62.8% on average, respectively. Correlation analysis showed that leaf dry weight, total fresh and dry weight, and large diameter of the plant had the highest correlation with essential oil yield. Stepwise regression analysis indicated that essential oil yield changes were mainly explained by leaf dry weight, essential oil content, total fresh weight, the number of stems and the amount of plant crown cover. In general, according to the results, the superiority of the N. assurgens species was significant and could be recommended as a valuable and high potential yielding plant for pharmaceutical purposes.
L. Safaei; H. Zeinali; D. Afiuni
Abstract
In order to study the effect of irrigation water salinity on the quantity and quality of fennel (Foeniculum vulgare Mill.) oil, and seed production, a field experiment was conducted in a split-plot based on randomized complete block design with three replications. The experimental treatments included ...
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In order to study the effect of irrigation water salinity on the quantity and quality of fennel (Foeniculum vulgare Mill.) oil, and seed production, a field experiment was conducted in a split-plot based on randomized complete block design with three replications. The experimental treatments included fennel genotype at four levels (German P11-820065, European 11486, and two indigenous genotypes including Lorestan and Hamedan) and water salinity at three levels (2 (control), 5, and 8 dS/m). The results showed that the effect of salinity was significant on all traits except α-pinene, myrcene, and essential oil percentage. Genotypes showed no significant difference in anethole, α-phellandrene, γ-terpinene, percentage, and essential oil yield. The interaction of salinity× genotype had a significant effect on all traits. Genotype P11-820065 had the highest essential oil percentage (3.90%) and yield (91.41 kg ha-1), and seed dry yield (2359.7 kg ha-1) between genotypes at control salinity level. With increasing salinity level, the seed and essential oil yield in non-indigenous genotypes decreased by a slope more than that of indigenous genotypes. Moreover, the content of anethole as the most important essential oil component of fennel increased to 5 dS/m salinity and then decreased. Based on the results of this study, under irrigation water salinity conditions, indigenous genotypes had higher economic efficiency due to their higher resistance to salinity stress. Water salinity affected only the content of essential oil components, but had no effect on its composition.
Y. Shakoori; B. Kashefi
Abstract
Considering the importance of medicinal properties of tarragon, and its propagation problems, tissue culture techniques can be used for its propagation. The aim of this study was to compare different culture media and chemical plant growth regulators on the growth traits and secondary metabolites of ...
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Considering the importance of medicinal properties of tarragon, and its propagation problems, tissue culture techniques can be used for its propagation. The aim of this study was to compare different culture media and chemical plant growth regulators on the growth traits and secondary metabolites of tarragon (Artemisia dracunculus L.). Treatments were culture medium at three levels of Murashige and Skoog (MS), Gamborg (B5) and Schenk & Hildebrandt (SH), and growth regulators at five levels of Kinetin (KIN), NAA, and 2,4-D alone (each with a concentration of 0.5 mg l-1), and two combinations of NAA+KIN and 2,4-D+KIN. The present study was conducted as a factorial based on completely randomized design with four replications. The results showed that culture media and growth regulators treatments had significant effects on callus characteristics, regenerated plantlets, callus secondary metabolites, and plant essential oil and its composition. MS medium caused a higher callus induction percentage and more increase in its fresh and dry weight. MS with NAA+KIN and 2,4-D+KIN caused the highest regeneration percentage (35.5), root length (1.28 cm), root (6.5 g) and stem (2.6 g) dry weight, the amount of callus phenolics, and also the amount of essential oil (2.42%) and ocimene (5.78%). Estragol percentage of essential oil increased by 2,4-D, while NAA enhanced ocimene, limonene, and linalool. The percentage of limonene in treatment with NAA alone and linalool in treatment with 2,4-D+ KIN was higher than those of other treatments. The results showed that the highest growth index, callus secondary metabolites, and plant essential oil were observed in MS medium containing growth regulators. Also, the application of kinetin with NAA and 2, 4-D resulted in improved callus growth, regeneration, essential oil, and some of its compounds production in tarragon.
M.H. Lebaschi; E. Sharifi Ashourabadi; M. Makizadeh Tafti; A.H. Talebpour; J. Hasani; Kh. Karimzadeh; S. Asadi sanam
Abstract
In order to study the effect of plant density on some quantitative and qualitative characteristics of Thymus pubescens Boiss. & Kotschy ex Celak under rainfed conditions, a field experiment was conducted in a randomized complete block design with three replications in three different dry farming ...
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In order to study the effect of plant density on some quantitative and qualitative characteristics of Thymus pubescens Boiss. & Kotschy ex Celak under rainfed conditions, a field experiment was conducted in a randomized complete block design with three replications in three different dry farming regions of the country during two years. Experimental treatments consisted of plant density (25, 35 and 50 cm between plants on rows equal to 8, 6 and 4 plant/m2, respectively), time (first and second year after planting) and location (East Azarbayjan, Kordestan, and Tehran). The traits including plant height, canopy diameter, dry matter yield of aerial parts, essential oil yield and content were measured at full flowering stage. The results of ANOVA indicated the significant effect of density, location and time and their interaction on all traits studied. The highest dry matter yield (1147 kg ha-1) was obtained in both two years in Tehran at 8 plant/m2. Kordestan with 2.25% and East Azarbayjan with 0.43% had the highest and lowest essential oil percentage, respectively. In density × location interaction, the highest amount of essential oil yield was obtained in Kordestan (21.73 kg ha-1) and Tehran (17.88 kg ha-1) at a density of 8 plant/m2. Thymus pubescens showed higher dry matter and essential oil yield under high plant density, indicating the establishment and adaptation of the species to different rainfed regions of the country especially Damavand and Kordestan.
B. Bahreininejad; M. Mirza
Abstract
Thymus daenensis Celak is one of the Iranian endemic plants. Since this species is one of the most valuable medicinal plants in the central Zagros region, extra-human exploitation has put it at risk of extinction. Ordination technique is one of the methods showing the relationship between plant and environmental ...
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Thymus daenensis Celak is one of the Iranian endemic plants. Since this species is one of the most valuable medicinal plants in the central Zagros region, extra-human exploitation has put it at risk of extinction. Ordination technique is one of the methods showing the relationship between plant and environmental variables. In order to study the effects of ecological variables on essential oil components, eight growing regions of Thymus daenensis in Isfahan province were identified and after collecting the aerial parts of plant materials, the essential oil was extracted and their components were detected. Twenty four bio-climatical, edaphical and topographical factors in each habitate were collected for ordination analysis. Results showed that elevation, mean temperature of the wettest season, isothermality, precipitation of the wettest season, annual precipitation, precipitation of the driest season and annual temperature range, clay, gravel, organic matter, available nitrogen and potassium were the most effective variables on the essential oil components. Thymol content as the main component in the essential oil was affected by annual temperature range, elevation, and slope. Soil sand percentage and the mean temperature of wettest quarter were the most effective variables on carvacrol. The result of this study could have valuable role in Thymus daenensis cultivation management in nature or farm regarding to its chemical components needed in the medicinal and hygienic industries.
M. Panahyan Kivi
Abstract
Due to the irrigation water crisis and the necessity of water resources management as well as the positive role of elements such as zinc in reducing the negative effects of drought stress, a field experiment was carried out as split plot based on randomized complete block design with three replications ...
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Due to the irrigation water crisis and the necessity of water resources management as well as the positive role of elements such as zinc in reducing the negative effects of drought stress, a field experiment was carried out as split plot based on randomized complete block design with three replications in Cooperative research field of Yashil Bakhar, Ardebil in 2017. In this research, yield and its components and essential oil of coriander (Coriandrum sativum L.) were investigated in reaction to zinc sulfate foliar spray under drought stress conditions. The experimental factors consisted of four levels of irrigation: irrigation after 70, 100, 130 and 160 millimeter evaporation from class A pan, and three levels of zinc sulfate foliar spray: control (water foliar spray) and foliar spray with three (Zn1:3 g ZnSO4/L) and six (Zn2: 6 g ZnSO4/L) per thousand concentrations. Irrigation and zinc sulfate foliar spray treatments were allocated to the main and sub plots, respectively. The results showed that drought stress reduced the number of umbels per plant, seeds per umbel, seeds per plant, and biological and seed yield. Zinc sulfate foliar spray at Zn2 level resulted in a significant increase in the number of umbels per plant (10.7), biological (21.1%) and grain (21.2%) yield. Under drought stress conditions, the percentage of coriander seeds essential oil increased by 49.2%, but the essential oil yield (1.02 kg ha-1) decreased. The essential oil yield was significantly increased by 27.4% with zinc sulfate foliar spray at Zn2 level. There was no statistically significant difference between Zn1 and Zn2 levels of zinc sulfate foliar spray. Therefore, 3 g ZnSo4/L foliar spray in areas with climatic conditions and soil similar to the research site is recommended to improve the yield and essential oil of coriander.
B. Shabani; R. Rezaei; H. Charehgani; A. Salehi
Abstract
Wheat bacterial blight disease caused by the bacterium Pseudomonas syringe pv. syringae (Pss) is one of the important diseases of wheat in the world. The expanding the diseases resistance to chemical compounds has led to the researches into the new antimicrobial agents finding. Studies have shown ...
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Wheat bacterial blight disease caused by the bacterium Pseudomonas syringe pv. syringae (Pss) is one of the important diseases of wheat in the world. The expanding the diseases resistance to chemical compounds has led to the researches into the new antimicrobial agents finding. Studies have shown that plant essential oils induce biological activity against plant pathogenic bacteria and can be used as an environment friendly and safe way to control the disease. These compounds have the least harm to the ecosystem and can be used as an alternative to toxic compounds for controlling plant diseases. This study was designed to evaluate the antimicrobial activity of the essential oils obtained from the aerial parts of Rosmarinus officinalis, Mentha spicata and Thymus daenensis, against Pss on wheat cultivars Pishtaz, Sirvan and Chamran under greenhouse conditions. In this study, all essential oil treatments significantly reduced the severity of wheat bacterial blight, especially on Pishtaz cultivar, and the percentage of necrotic area on the leaf 30 days after bacterial inoculation. The highest reduction in the severity of the disease was obtained by Mentha spicata and Thymus daenensi essential oils. The findings of the present study suggest that essential oils of Rosmarinus officinalis, Mentha spicata and Thymus daenensis are capable to be used as antimicrobial agents.
