Phytochemistry (extraction, identification and measurement of active components)
S. Parsafar; Gh. Eghlima; M.H. Mirjalili; S. Nejad Ebrahimi; J. Hadian
Abstract
The morphological, yield, and phytochemical characteristics of Solidago virgaurea L. were studied at the full flowering stage in two habitats of Pol Sefid and Sang Deh in Mazandaran province in 2019. The traits including plant height, plant diameter, inflorescence length, number of stems, length and ...
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The morphological, yield, and phytochemical characteristics of Solidago virgaurea L. were studied at the full flowering stage in two habitats of Pol Sefid and Sang Deh in Mazandaran province in 2019. The traits including plant height, plant diameter, inflorescence length, number of stems, length and leaf width, length and ray floret width, tubular floret length, flower diameter, receptacle diameter, stem diameter, fresh and plant dry weight, fresh and leaf dry weight, fresh and stem dry weight, fresh and leaf+flower dry weight, and total phenols (by Folin-Ciocalteu method), flavonoids (by aluminum chloride method), and leiocarposide (by HPLC) contents were measured. The results showed that the highest amounts of inflorescence length (40 cm), flower diameter (21.1 mm), plant dry weight (9.44 g plant-1), flower dry weight (6.06 g plant-1), flower+leaf dry weight (7.22 g plant-1), and stem dry weight (2.22 g plant-1) were obtained in the population "Pol Sefid". A positive and significant correlation was observed between the plant dry weight and traits including the plant height, number of stems, stem diameter, flower diameter, plant fresh weight, fresh and leaf dry weight, fresh and stem dry weight, fresh and leaf+flower dry weight, and total flavonoids. The content of total phenols (26.95 mg Gallic acid/g DW), total flavonoids (8.82 mg Rutin/g DW), and leiocarposide (2.08 mg/g DW) was higher in the population "Sang Deh" than "Pol Sefid".
Y. Sepahi Sarjo; S.M. Mousavu Nik; M. Galavi; A. Ghanbari; A.Sh. Raissi; F. Nosrati
Abstract
Didar (Euphorbia tirucalli L.) from the family Euphorbiaceae, is a perennial succulent shrub with broad branched and round branches. Environmental factors are one of the most important factors affecting qualitative and quantitative traits in medicinal plants. In this study, Didar was studied in different ...
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Didar (Euphorbia tirucalli L.) from the family Euphorbiaceae, is a perennial succulent shrub with broad branched and round branches. Environmental factors are one of the most important factors affecting qualitative and quantitative traits in medicinal plants. In this study, Didar was studied in different habitats of Sistan and Baluchestan province in terms of some morphological, physiological, soil, and phytochemical characteristics. This study was conducted as a nested design and Duncan's least significant differences test at 5% level with completely randomized design with three replications using field survey and included some cities: 1- Hichan district from Nikshahr city (Kalat, Hesarak and Hashtik villages), 2- Rask city (Firoozabad, Jangal and Parood villages) and 3- Sarbaz city (Hit, Kishkour and Pirdan villages). The results showed that there was a significant difference between all the studied traits among cities and regions. In this study, morphological traits had the highest amount in Nikshahr city and the lowest height and number of branches belonged to Sarbaz city, indicating that the vegetative growth of this shrub decreases with increasing altitude. The soil studied in Nikshahr city regions was more favorable than other areas in terms of key elements such as phosphorus and potassium. The highest levels of 1- chlorophylls a and b and carotenoids and 2- proline and flavonoids were measured in spring and summer, respectively, but the highest amount of anthocyanin was observed in different cities variably. The highest amounts of 1- chlorophylls a and b and 2- carotenoids and anthocyanin were measured in Sarbaz and Rask cities, respectively. The highest amount and number of active ingredients of leachates of this plant were observed in summer.
J. Motamedi; E. Sofi Khajavi; A. Alijanpour; E. Sheidai Karkaj
Abstract
Study on the habitat characteristics of medicinal plants and estimating their production is one of the basic requirements for designing a future perspective document for utilization of by-products. Salep (Orchis palustris Jacq.) is one of the most important and highly valuable medicinal plants having ...
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Study on the habitat characteristics of medicinal plants and estimating their production is one of the basic requirements for designing a future perspective document for utilization of by-products. Salep (Orchis palustris Jacq.) is one of the most important and highly valuable medicinal plants having a significant distribution in the meadows of Targavar region of Urmia. Hence, among the meadows, six locations that were easier to reach and the distribution of Salep was more uniform were selected. In each location, using 60 plots of 60×25 cm2 that were randomly located within 10 plots with 10×1 m2, the number of Salep bases were counted and traits including crown cover percentage, plant height and tuber production were measured. Three composite soil samples from each location were randomly taken from the depth of root development of dominant species, and soil characteristics including sand, silt, clay, acidity, electrical conductivity, organic matter and total nitrogen were measured. The depth of groundwater (in each location) was considered as a criterion for moisture level and altitude, to analyze the relationship between habitat characteristics and plant traits. For this purpose, based on the calculated gradient length, the redundancy analysis (RDA) method was used as a linear method. The results of RDA showed that plant traits, especially tuber weight, were directly affected by the amount of organic matter, organic carbon, total nitrogen and clay percentage of the habitats. The mentioned traits are reversely related with altitude, groundwater depth and soil acidity of locations. In general, the Salep species more prefer low-altitude habitats such as alluvial plains with fertile, neutral acidity and heavy texture soils and high groundwater surface.
M. Yousefi; V. Nazeri; M. Mirza
Abstract
In this study, 12 populations of Salvia leriifolia Benth. were collected to investigate the effects of environmental factors on the quantity and quality of essential oil. Oil was extracted using hydrodistillation based on the British Pharmacopoeia method. Chemical compositions of essential oils were ...
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In this study, 12 populations of Salvia leriifolia Benth. were collected to investigate the effects of environmental factors on the quantity and quality of essential oil. Oil was extracted using hydrodistillation based on the British Pharmacopoeia method. Chemical compositions of essential oils were detected using the GC-FID and GC-MS techniques. The influence of environmental factors on essential oil and chemical composition was analyzed using canonical correlation by SAS v. 9.1. Results showed that the average yield of essential oil were between 0.69-1.5% (v/w) for Tabas Sabzevar and Bajestan, respectively. Twenty-two compounds were identified, among which the concentration of juniper camphor (12.0-39.9%), 1,8-cineole (3.6-21%), β-pinene (8.7-18.1%) and α-pinene (4.4-10%) was high. Canonical correlation analysis among eight morphological traits and nine chemical properties revealed that the first six canonical variables had a significant canonical correlation. Canonical correlation coefficients of the first variable showed that high levels of α-pinene and β-pinene and low percentage of δ-cadinole had a direct and positive relationship with low amounts of soil calcium and potassium. In other words, the cultivation of S. leriifolia in soils rich in calcium and potassium increases δ-cadinole and α-pinene content and decreases β-pinene content in the essential oil.
M. Bigdeloo; V. Nazeri; J. Hadian
Abstract
Thymus caramanicus Jalas is belonging to family Lamiaceae. Among 250 species of this genus with a worldwide distribution, 14 species grow naturally in Iran. Thymus caramanicus Jalas is one of the endemic species which has distribution in central Iran. In this study, natural habitats of the species were ...
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Thymus caramanicus Jalas is belonging to family Lamiaceae. Among 250 species of this genus with a worldwide distribution, 14 species grow naturally in Iran. Thymus caramanicus Jalas is one of the endemic species which has distribution in central Iran. In this study, natural habitats of the species were identified in different regions of Kerman, Esfahan and Semnan provinces in 2010.Ten complete plants at flowering stage were collected from seven localities in order to study and evaluate the morphological characters. Flowering stems were used to extract essential oil. Characteristics associated with each habitat were recorded and three soil samples from each site were taken to evaluate soil physical and chemical properties. Vegetative and reproductive characteristics of each population and the essential oil of the studied species in each habitat were recorded. Results were analyzed using cluster analysis method with SPSS software and correlation between evaluated traits and essential oil productivity were determined. Populations were clustered in two groups based on all studied characters. Populations collected from Kerman and Esfahan provinces were located in a same group and populations from Semnan province were placed in an independent group. Differences between these two groups may be from different climates in their habitats and their adaptation to the different environmental factors. According to the results, the best vegetative and also reproductive characters such as flowering stem length, leaf length and width, number of flowers per inflorescence and corolla length and also the highest essential oil production (2-3%) were recorded for specimens from Kerman province; therefore, it is recommended for breeding programs or cultivation.
