Improvement and breeding
Farahnaz Houshidari; Seyed Reza Tabaei-Aghdaei; Fatemeh Sefidkon; Bayzid Yousefi
Abstract
Abstract: Savory belongs to the family Lamiaceae and genus Satureja. Among the 16 species of savory in Iran, 9 are endemic to Iran. In this study, we evaluated the adaptation of different savory accessions (Satureja spp.) in the climatic condition of Kurdistan province. For this purpose, we studied 37 ...
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Abstract: Savory belongs to the family Lamiaceae and genus Satureja. Among the 16 species of savory in Iran, 9 are endemic to Iran. In this study, we evaluated the adaptation of different savory accessions (Satureja spp.) in the climatic condition of Kurdistan province. For this purpose, we studied 37 accessions belonging to 10 species of Satureja in an experiment with a randomized complete block design in three replications at the Grize Station in Sanandaj during 2009-2013. Savory species include S.sahendica Bornm, S. avromanica Maroofi, S. bachtiarica Bunge, S. spicigera (C. Koch) Boiss, S. mutica Fisch, S. macrantha CAMey, S. atropatana Bunge, S. rechingeriJamzad ,S. khuzistanica Jamzad, S.isophylla Rech. First, we collected the seeds of 37 populations from the Satureja spp. from different regions of Iran as a part of a large project executed by experts in the Iranian Research Institute of Forests and Rangelands. Then we planted the seeds using transplanting trays and finally at the stage of 8-10 leaves, the healthy plants were selected to be cultivated in special pots. Based on the results of variance analysis there was a significant difference between species and accessions at the level of (p≤0.01) in terms of essential oil yield percentage, essential oil yield per hectare (based on the dry weight of flower and leaf per hectare), total phenolic compounds of essential oil, plant’s fresh weight yield per hectare and plant’s dry weight yield per hectare, dry weight of leaf and flower yield per hectare, canopy area, the mean of the branch’s height and establishment percentage. Also, the effect of year on essential oil yield was significant.
Phytochemistry (extraction, identification and measurement of active components)
M. Jafari Ghoshchi; B. Abbaszadeh; M. Ourei; R. Azimi; A. Faramarzi
Abstract
In this study, to investigate the effects of gamma ray to dry plant materials, conditions, and storage duration of the plants on the percentage and essential oil composition and some physiological traits of Satureja spicigera (C. Koch) Boiss., plant top shoots were collected at the flowering stage. Gamma ...
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In this study, to investigate the effects of gamma ray to dry plant materials, conditions, and storage duration of the plants on the percentage and essential oil composition and some physiological traits of Satureja spicigera (C. Koch) Boiss., plant top shoots were collected at the flowering stage. Gamma ray irradiation was performed in the Atomic Energy Organization at five levels of 0, 2.5, 5, 7.5, and 10 kGy (kilo gray). Storage was carried out under two refrigerator (4°C) and room air temperature conditions at three storage duration levels of 0, 120, and 240 hours. The experiment was conducted as a factorial in a completely randomized design with three replications. Essential oil of flowering shoots was extracted by water distillation method and analyzed by GC and GC/MS quantitatively and qualitatively. According to the obtained results, gamma radiation affected the essential oil percentage and amount of p-cymene, thymol, carvacrol, phenols, flavonoids, and antioxidant activity significantly at the level of 1%. Storage duration had a significant effect on the essential oil percentage and amount of p-cymene, thymol, and carvacrol. Storage conditions showed a significant effect on the total phenols and flavonoids content and antioxidant activity of ethanol extract of S. spicigera. Means comparison of gamma radiation effects showed that the highest percentage of essential oil with 1.12% and 1.03% was obtained in the control and 5 kGy treatments, respectively. The highest amount of thymol (13.57%) and carvacrol (34.73%) was observed in the 10 kGy and control treatments, respectively. With 2.5 kGy gamma irradiation, the amount of phenolic compounds and antioxidant activity respectively increased by 1.4 and 1.76 times compared to the control. Of course, by keeping the plant in the refrigerator, the amount of thymol was reduced. The highest percentage of essential oil was related to the storage for 120 hours. The use of high intensity gamma radiation is suitable to achieve high thymol essential oil. The use of low radiation can also be useful for drying S. spicigera to obtain extracts containing more phenolic compounds. However, gamma radiation is not appropriate for plant materials drying to obtain essential oils of this species.
