Agriculture and horticulture
M. Babaei; F. Sefidkon; M. Nasiri
Abstract
Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete ...
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Two species Nepeta cataria L. and Nepeta bracteata Benth. (fam. lamiaceae) are native to Iran. To study the quantity and quality of the two species essential oil in the field, the seeds of eight populations from these two species were collected from natural habitats and planted in a randomized complete block design in Alborz Research Station of Research Institute of Forests and Rangelands (Karaj, Alborz province). The flowering branches were harvested at the full flowering stage and after room temperature-drying, their essential oils were extracted by hydrodistillation, and analyzed and identified by GC and GC/MS. The essential oil yield of N. cataria populations varied between 0.02 (Karaj) and 0.50% (Arak). Twenty-three compounds were identified in the essential oil of this species, and the main compound in all populations was from nepetalactone isomers. NepetalactoneIII (4aα,7β,7aα-nepetalactone) and nepetalactoneI (4aα,7α,7aα-nepetalactone) constituted 44.4 (Karaj) to 91.6% (Arak) and 0.8 (Karaj) to 15.9% (Bafgh1) of the essential oil, respectively. NepetalactoneII (4aα,7α,7aβ-nepetalactone) was observed only in the population Bafgh2 (21.2%). The amount of 1,8-cineole in the essential oil of different N. cataria populations varied from 0.4 (Taft1) to 12.8% (Karaj). The essential oil yield of N. bractaeta populations was obtained between 0.02 (Ardakan) and 0.70% (Taft2). Twenty-seven compounds were identified in the essential oil of this species, and the main compounds were 1,8-cineole (1.0, 9.6, and 41.0% in Tabas, Ardakan, and Taft2, respectively) and geranyl acetate (0.9, 3.4, and 39.8% in Tabas, Taft2, and Ardakan, respectively). In general, the results showed that the N. cataria populations were all from the same chemotype, but N. bracteata populations were from two chemotypes (ct. geranyl acetate and ct. 1,8-cineole).
A. Baghizadeh; Z. Mashayekhi; M.A. Ebrahimi
Abstract
Catnip (Nepeta cataria L.), also called catmint, is a medicinal plant containing aromatic compounds and belongs to the Lamiaceae family. In this study, phytochemical and genetic variations of some Catnip germplasm were analyzed using RAPD molecular marker as well as GC/MS. The shoots of plants were collected ...
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Catnip (Nepeta cataria L.), also called catmint, is a medicinal plant containing aromatic compounds and belongs to the Lamiaceae family. In this study, phytochemical and genetic variations of some Catnip germplasm were analyzed using RAPD molecular marker as well as GC/MS. The shoots of plants were collected from various habitats and dried away from direct sunlight. DNA was extracted using (CTAB) method. Eleven RAPD primers were used in PCR reaction. Based on the results of cluster analysis, 15 populations were classified into four groups. Based on the results of principal component analysis, the plant populations were clustered into five groups. Eight of 15 collected populations were analyzed phytochemically. Essential oils were extracted via water steam distillation using Clevenger apparatus. Twenty-seven components were identified from all essential oil samples. The results of GC/MS method showed that among the samples, 100% of essential oil compounds were identified in populations of Sirch, Mohammad Abad-e-Maskoon, Saqder 2, Dalfard 2, and Mijan 2. The results also showed that in samples of Dehbakri, Dalfard 1, and Saqder 1, 94.84%, 99.8% and 96.6% of essential oil compounds were identified, respectively. Three isomers of Nepetalactone constituted the major essential oils compounds. B-caryophyllene, Caryophyllene oxide, β-pinene, and α-pinene were also of main essential oil compounds. Based on the cluster analysis of GC/MS data, the populations were classified into three groups. Comparison of consisting compounds of essential oils in the eight studied populations indicated that the essential oils extracted were different quantitatively and qualitatively. This could be probably due to the different ecological conditions of habitats such as humidity, temperature, altitude above sea level, or other terrestrial, geographical and genetic factors.