Agriculture and horticulture
Kosar Taheri Boukani; Abbas Hassani; MirHassan Rasouli-Sadaghiani; Mohsen Barin; Ramin Maleki
Abstract
Background and objectives: Basil (Ocimum basilicum L.) is an annual herbaceous plant belonging to the mint family (Lamiaceae). Its fresh and dried leaves and essential oils are used in the food, pharmaceutical, and cosmetic industries. In recent years, the use of symbiosis with plant beneficial rhizospheric ...
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Background and objectives: Basil (Ocimum basilicum L.) is an annual herbaceous plant belonging to the mint family (Lamiaceae). Its fresh and dried leaves and essential oils are used in the food, pharmaceutical, and cosmetic industries. In recent years, the use of symbiosis with plant beneficial rhizospheric microorganisms including rhizobacteria and endophytic fungi has been considered as a cost-efficient and sustainable strategy to alleviate the adverse effects of environmental stresses such as salinity. Therefore, the present study was conducted in the research greenhouse of Urmia University. It evaluated the effect of inoculation with growth-promoting microorganisms on some growth, physiological, and phytochemical characteristics of basil plants under salt-stress conditions.Methodology: This research was conducted as a factorial experiment in a completely randomized design with three replications. The experimental factors were inoculation with microorganisms at three levels (control without inoculation, inoculation with Serendipita indica, and inoculation with a mixture of Pseudomonas areuginosa, P. putida, and P. fluorescens) and salinity stress at four levels (0, 40, 80 and 120 mM of NaCl). For inoculation, the germinated seeds were separately inoculated with S. indica suspension (5×105 spores per ml) and inoculum containing a mixture of Pseudomonas bacteria (1.61×109 cells per ml) and planted in prepared pots. The pots were irrigated with ordinary tap water until the plants were eight-leafed, and from this stage onwards, salinity stress treatments were applied by dissolving different concentrations of NaCl in the irrigation water and continued until the full flowering stage. In the full flowering stage, plant samples were collected and root colonization percentage by fungus, growth parameters (plant height, stem diameter, number and total length of lateral branches, leaf number and area, inflorescence length, fresh and dry weight of leaf and stem), leaf relative water content (RWC), photosynthetic pigments, concentration of leaf nutrients (N, P, K, Na and Cl), essential oil content and yield were evaluated. For essential oil extraction, shade-dried samples were hydro-distilled using a Clevenger-type apparatus.Results: The results showed that due to salinity stress, the percentage of root colonization by S. indica, growth parameters, RWC, photosynthetic pigments, essential oil content, yield, N, P, and K content, and K/Na ratio decreased while Na and Cl content increased. In addition, all evaluated parameters in inoculated plants were higher than in non-inoculated plants except Na and Cl content. The highest and lowest rates of growth parameters, RWC (74.47 and 72.39%), essential oil yield (0.23 and 0.17 ml/pot), and N content of leaves (1.36 and 1.14%) were obtained in plants inoculated with S. indica and non-inoculated plants, respectively. The highest and lowest amounts of chlorophyll a (0.87 and 0.74 mg/g fw), chlorophyll b (0.41 and 0.37 mg/g fw), essential oil percentage (1.24 and 1.05%), K content (5.15 and 3.97%), P content (0.29 and 0.24%) and the K/Na ratio (10.05 and 4.07), were observed in plants inoculated with a mixture of Pseudomonas bacteria and non-inoculated plants, respectively. Also, the lowest Na (1.34%) and Cl (2.93%) accumulation was observed in plants inoculated with Pseudomonas bacteria and S. indica fungus, respectively.Conclusion: According to the results of this study, the use of plant growth-promoting microorganisms (S. indica fungus and a mixture of Pseudomonas bacteria) can alleviate adverse effects of salinity stress on the growth and essential oil production of the basil plant by increasing the absorption of water and nutrients, preserving photosynthetic pigments and reducing the accumulation of toxic ions.
M. Ghorbanli; F. Ahmadi; A. Monfared; Gh. Bakhshi Khaniki
Abstract
Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and ...
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Ascorbate as a strong antioxidant has a considerable bio effect on growth of plants, such as increase in their tolerance against environment stresses. In this investigation, the effect of salt stress and its interaction with ascorbate on amount of proline, catalase and ascorbate peroxidase enzymes and MDA in a medicinal plant of Cuminum cyminum L. was randomly studied in the green house conditions with three replications. Plants were treated by different concentrations of NaCl (0, 25, 50, 75, 100, 125 mmolar) and ascorbate (9 mmolar). In the plants treated with salt, the amount of proline, antioxidant enzymes activity and MDA were increased with increasing NaCl concentrations. The plants treated with NaCl and ascorbate at the same time in a same NaCl concentration, the amount of proline, catalase activity, ascorbate peroxidase and MDA were increased. The results indicated that the ascorbate was one of the antioxidants that caused an increase in resistance of Cuminum cyminum L. to salt stress.
A. Farzaneh; M.T. Ebadi; S.H. Nemati; H. Arouiee
Abstract
In order to investigate the germination factors of two improved cultivars and one Iranian landrace of cornflower (Centaurea cyanus) under salt stress conditions a factorial experiment based on completely randomized design with two factors in three replications was conducted at experimental laboratory ...
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In order to investigate the germination factors of two improved cultivars and one Iranian landrace of cornflower (Centaurea cyanus) under salt stress conditions a factorial experiment based on completely randomized design with two factors in three replications was conducted at experimental laboratory of department of horticulture, Ferdowsi University of Mashhad during 2009. The main factor included two improved cultivars of cornflower (Ball blue and Ball junge) and one Iranian landrace and second factor included seven salinity levels (0, 50, 100, 150, 200, 250 and 300 milli molar NaCl). Measured characteristics were germination percentage, germination velocity, vigor index, radicle and plumule length. According to the results of ANOVA, different levels of salt stress had significant effects on all measured characteristics while the effect of cultivar type was only significant on germination percentage, germination velocity and vigor index. The interaction between salinity and cultivar type had significant effect on all measured characteristics. The results showed that with increase in salinity level, all measured characteristics were significantly decreased. The most germination percentage and germination velocity (51.04 percent and 11.82 seed/day respectively) were obtained from Ball blue in control treatment and the lowest (9.32 percent and 0.5 seed/day respectively) was obtained from Iranian landrace and Ball junge cultivar in 300 mili molar NaCl. Decrease of plumule length was more than that of radicle length under salinity stress. In this study, improved cultivars of cornflower and Iranian landrace showed different responses to salt stress due to the diversity in their genotypes. Generally, Ball blue was identified as the most salt tolerance cultivar in germination stage.
A. Dadkhah
Abstract
This experiment was conducted in germinator in order to study the effects of water potential on seed germination, germination rate and seedlings growth of four medicinal plants as Trigonella foeaum-graecum L., Sesamum indicum L., Cannabis sativa L. and Carum copticum (L.) C. B. Clarke. Four water potential ...
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This experiment was conducted in germinator in order to study the effects of water potential on seed germination, germination rate and seedlings growth of four medicinal plants as Trigonella foeaum-graecum L., Sesamum indicum L., Cannabis sativa L. and Carum copticum (L.) C. B. Clarke. Four water potential were used including distilled water as control (0), -0.37, -0.59 and -0.81 Mpa which has been made by different salts (NaCl, CaCl2 and NaCl+CaCl2 in 5 to 1 molar ratio). The experiment was carried out based on completely randomized design with six replications. Results of variance analysis showed that effects of water potential, type of salt composition on germination percentage, rate of germination, root and shoot length were significant. With decreasing water potential, germination rate and percentage decreased. Of cource plants had different responses such as Sesamum indicum was not affected by decreasing water potential where as other significantly were decreased. The effect of salt composition was significant on rate and percentage of germination. The percentage of germination at lower water potential which was made by NaCl+CaCl2 significantly was higher than the same water potential made by only NaCl and CaCl2.
O. Khorsandi; A. Hassani; F. Sefidkon; H. Shirzad; A.R. Khorsandi
Abstract
Water and soil salinity on the environmental agents limit plant growth and its productivity in Iran. Anise Hyssop (Agastache foeniculum kuntz.) is a perennial and aromatic herb plant, belonging to the Lamiaceae family. The essential oil of Anise Hyssop used in food industries, pharmacy, perfumery and ...
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Water and soil salinity on the environmental agents limit plant growth and its productivity in Iran. Anise Hyssop (Agastache foeniculum kuntz.) is a perennial and aromatic herb plant, belonging to the Lamiaceae family. The essential oil of Anise Hyssop used in food industries, pharmacy, perfumery and making soda. This experiment was conducted in a randomized complete blocks design with six salt treatments including 0 (control), 25, 50, 75, 100 and 125 mM NaCl and four replications in green house. Some parameters such as growth, yield and content and composition of essential oil were modulated. The results showed that salt stress had significant effects on estimated parameters. Salinity decreased plant height, number and length of axillary shoots, steam diameter, length of internodes, number and area of leaves, fresh and dry weight of leave and shoot, herbal yield and amount of essential oil in pot. In the composition of essential oil β-pinene, myrcene, anisaldehyd and β-bourbonene increased and amount of linalool and methyl chavicol decreased. Also high salinity (100 and 125 mM) destroyed plants.
I. Khammari; Sh.A. Sarani; M. Dahmardeh
Volume 23, Issue 3 , November 2007, , Pages 331-339
Abstract
This study was conducted to evaluate salt tolerance in Cyamopsis psoraloides, Cynara scolymus, Hibiscus sabdariffa, Cassia angustifolia, Ocimum basilicum and Hyssopus officinalis at germination stage. Salinity levels, including 0, 50, 100, and 200 mM were applied using a completely randomized design ...
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This study was conducted to evaluate salt tolerance in Cyamopsis psoraloides, Cynara scolymus, Hibiscus sabdariffa, Cassia angustifolia, Ocimum basilicum and Hyssopus officinalis at germination stage. Salinity levels, including 0, 50, 100, and 200 mM were applied using a completely randomized design with three replications. Germination percentage and rate were measured in the Petri dishes and root and shoot lengths were recorded at seeding stage. There was no germination for Hibiscus sabdariffa, Cassia angustifolia, Ocimum basilicum and Hyssopus officinalis at 200 mM NaCl. However, Cyamopsis psoraloides and Cynarascolymus showed seed germination at 200 mM NaCl. Higher salt tolerance potential in some species makes cultivation possible or other stress tolerant medicinal plants under saline environments.
S.F. Afzali; H. Shariatmadari; M.A. Hajabbasi; F. Moatar
Volume 23, Issue 3 , November 2007, , Pages 382-390
Abstract
Flavonoids are polyphenolic compounds with strong antioxidant effects. Chamomile (Matricaria chamomilla L.) is used for a variety of herbal remedies, containing classes of biologically active compounds including flavonoids and essential oil. There have also been many reports, showing that flavonol-O-glycosides ...
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Flavonoids are polyphenolic compounds with strong antioxidant effects. Chamomile (Matricaria chamomilla L.) is used for a variety of herbal remedies, containing classes of biologically active compounds including flavonoids and essential oil. There have also been many reports, showing that flavonol-O-glycosides are quantitatively the most abundant flavonoids found in chamomile flowers. Tolerance of this plant on saline and drought conditions has not been well studied and documented. Therefore a greenhouse experiment was conducted using complete randomized design with four replications in order to evaluate the effects of salinity and drought stress on flower dry weight and falavonoids component of chamomile. Hydroponic solutions are used for salinity experiment at five levels of NaCl concentrations (0, 40, 80, 120, 190 mM). In order to examine drought stress, we used gravitational method and four treatments (FC soil moisture content, and 75, 90, 95 depletion percent of soil AWC) in a pot experiment. Then the flower dry weight and their flavonol-O-glycosides were measured. The Results showed that both salinity and drought reduced flower dry weight and flavonoids concentration, but drought reduction effects were more than salinity. Flowers dry weight significantly increased with increasing NaCl concentration up to 40 mM while decreased at higher salinity levels. There were no significant differences between salinity treatments up to 80 mM NaCl for flavonol-O-glycosides concentration. Regarding the dry flower yield, the highest content of flavonol-O-glycosides was observed in 40 mM NaCl treatments. Flower dry weight and Flavonol- O- glycosides decreased as drought levels increased.
