Biological effects of essential oils and extracts
P. Shiri; A. Arasteh; G. Tajadod
Abstract
Plantago major L. is a herbaceous perennial plant with many biological effects such as strong antioxidant properties. This study aimed at investigating the inhibitory effect of P. major seeds extract on amyloid nanofibrils production and also to antioxidant properties of this extract. First, the plant ...
Read More
Plantago major L. is a herbaceous perennial plant with many biological effects such as strong antioxidant properties. This study aimed at investigating the inhibitory effect of P. major seeds extract on amyloid nanofibrils production and also to antioxidant properties of this extract. First, the plant powdered-seeds hydroalcoholic extract was prepared using 96% ethanol. The extract compounds were then analyzed by gas chromatography-mass spectrometry (GC/MS) method. The production of amyloid fibrils was confirmed using the concord spectrometry method and transmission electron microscope imaging and its antioxidant effects were evaluated by the DPPH method. The presence of pyrrolidinone (2.95%) and N-(4-aminobutyl) acetamide (17.98%) in the extract was confirmed by GC/MS. The highest antioxidant activity (99.18%) and lowest amyloid nanofibrils production (55.73%) were obtained in the concentrations of 2 and 0.4 mg.ml-1 of the extract, respectively. The presence of amyloid fibrils was confirmed by electron microscopic imaging. The results of this research showed that the P. major seeds extract can be introduced as a suitable candidate to reduce the complications caused by Alzheimer's disease.
J. Mazinani; J. Vatandoost; M.R. Vaezi Kakhki; F. Ghorat
Abstract
Broadleaf plantain (Plantago major L.) is a herbaceous wild plant of the Plantaginaceae family with 10 to 45 cm in height and without stems. It grows in a wide area of Europe and Asia continents, as well as North Africa and North America, and in Iran almost everywhere. The seeds of the plant stop bleeding ...
Read More
Broadleaf plantain (Plantago major L.) is a herbaceous wild plant of the Plantaginaceae family with 10 to 45 cm in height and without stems. It grows in a wide area of Europe and Asia continents, as well as North Africa and North America, and in Iran almost everywhere. The seeds of the plant stop bleeding and are known as a plant source that plays an important role in at least one of the coagulation pathways. Therefore, this study was designed and implemented to evaluate the in vitro effect of ethanolic extract of broadleaf plantain seeds on human coagulation tests. The broadleaf plantain seeds were ground in a mortar and extracted in ethanol 80% for 48 hours. Extracts were dealcoholized by a rotary evaporator at 50 °C and the remaining water was removed from the oven at 40°C. The obtained plant dry extract was prepared in different concentrations in Owren-Koller bufferand their effects on coagulation indices including prothrombin time (PT), activated partial thromboplastin time (APTT) and clotting time (CT) were evaluated and the results were analyzed by t-test. The results showed that the ethanolic extract of broadleaf plantain seeds reduced APTT, but had no significant effect on PT and CT. Although the ethanolic extract of broadleaf plantain seeds showed the coagulation effect in this study, further studies on animal and human models seem necessary.
M. Ghorbanli; A. Sateei; S. Nasiri Savadkohi
Abstract
Plantago species generally are grassy plants that belong to Plantaginaceae, with large lamina on the ground surfaces. Plantago major L. grows widely in Asia, Europea, Africa, and North American and contains chemical compounds such as polysaccharides, lipids, coffeic acid derivatives, flavonoids, terpenoids, ...
Read More
Plantago species generally are grassy plants that belong to Plantaginaceae, with large lamina on the ground surfaces. Plantago major L. grows widely in Asia, Europea, Africa, and North American and contains chemical compounds such as polysaccharides, lipids, coffeic acid derivatives, flavonoids, terpenoids, ascorbic acid, benzoic acid, ferrolic acid, galactoronic acid and so on. In this study, Plantago major L. plants were cultured in several pots irrigated with hougland solutions and after 20 days, during 3 leaves stage, irrigation was continued with hougland solutions containing different concentrations of CuSO4, 0 (control), 100, 300, 500, 700 and 100µM. Antioxidant enzymes activities (catalase, peroxidase, poly phenol oxidase, ascorbate peroxidase) and phenolics content in leaves and roots were measured. The aim of the study was the effect of copper concentrations on antioxidant enzymes and phenolic compounds content in plantago major L. The result shows that by increasing concentration of copper in the medium, activity of catalse, ascorbate peroxidase and peroxidase in the root and leaves decreased significantly (0.05) in comparison with control. Polyphenol oxidase enzyme activity in roots and leaves increased, that was only significant in roots. Phenolics content increase in roots that was not significant but the decrease in leaves was significant.
